Comparison of methods for isolation of protozoa from ruminal fluid

Canadian Journal of Animal Science

Volumn 76, Issue 3, 1996, Pages 481-483

  Neill, L ^a   Ivan, M ^a  

^a AGRICULTURE AND AGRI FOOD CANADA   (Canada)

Author keywords

Filtration; Protozoal isolation; Ruminal fluid; Sedimentation

Indexed keywords

BACTERIA (MICROORGANISMS); PROTOZOA;

EID: 0030515661     PISSN: 00083984     EISSN: None     Source Type: Journal    
DOI: 10.4141/cjas96-073     Document Type: Article

References (6)

1. Dehority, B. A. and Grubb, J. A. 1980. Effect of short-term chilling of rumen contents on viable bacterial numbers. Appl. Environ. Microbiol. 39: 376-381.
2. John, A. and Ulyatt, M. J. 1984. Measurement of protozoa, using phosphatidyl choline, and of bacteria, using nucleic acids, in the duodenal digesta of sheep fed chaffed lucerne hay (Medicago sativa L.) diets. J. Agric. Sci. (Camb.) 102: 33-44.
3. Masson, M. J., Iggo, A., Reid, R. S. and Mann, G. F. 1952. Galvanotropic fractionation of rumen ciliatcs. J. R. Microsc. Soc. 72: 67-69.
4. Olubobokun, J. A., Craig, W. M. and Nipper, W. A. 1988. Characteristics of protozoal and bacterial fractions from microorganisms associated with ruminai fluid or particles. J. Anim. Sci. 66: 2701-2710.
5. Veira, D. M., Ivan, M. and Jui, P. Y. 1983. Rumen ciliate protozoa: Effects on digestion in the stomach of sheep. J. Dairy Sci. 66: 1015-1022.
6. Williams, A. G. and Yarlett, N. 1982. An improved technique for the isolation of holotrich protozoa from rumen contents by differential filtration with defined aperture textiles. J. Appl. Bacteriol. 52: 267-270.