Purification of recombinant adeno-associated virus by iodixanol gradient ultracentrifugation allows rapid and reproducible preparation of vector stocks for gene transfer in the nervous system

Human Gene Therapy

Volumn 10, Issue 11, 1999, Pages 1885-1891

  Hermens, Wim T J M C ^a   Brake, Olivier Ter ^a   Dijkhuizen, Paul A ^a   Sonnemans, Marc A F ^a   Grimm, Dirk ^b   Kleinschmidt, Jürgen A ^b   Verhaagen, Joost ^a,c  

^a VU UNIVERSITY MEDICAL CENTER   (Netherlands)

^b GERMAN CANCER RESEARCH CENTER DKFZ   (Germany)

^c NETHERLANDS INSTITUTE FOR NEUROSCIENCE   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

CESIUM CHLORIDE; IODIXANOL; VIRUS VECTOR;

ADENOVIRUS; ARTICLE; CONCENTRATION (PARAMETERS); CYTOPATHOGENIC EFFECT; DESALINATION; GEL ELECTROPHORESIS; GENE EXPRESSION; GENE TRANSFER; HUMAN; HUMAN CELL; NERVOUS SYSTEM; REPRODUCIBILITY; TRANSGENE; ULTRACENTRIFUGATION; VIRUS PURIFICATION; VIRUS RECOMBINANT;

ANIMALS; BRAIN; CENTRIFUGATION, DENSITY GRADIENT; CESIUM; CHLORIDES; CONTRAST MEDIA; DEPENDOVIRUS; GENE TRANSFER TECHNIQUES; GENETIC VECTORS; IMMUNOHISTOCHEMISTRY; NERVOUS SYSTEM; RATS; SPINAL CORD; TRIIODOBENZOIC ACIDS;

ADENO-ASSOCIATED VIRUS; ADENOVIRIDAE;

EID: 2442737801     PISSN: 10430342     EISSN: None     Source Type: Journal    
DOI: 10.1089/10430349950017563     Document Type: Article

References (19)

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