Infiltrating leukocytes confound the detection of E-cadherin promoter methylation in tumors

Biochemical and Biophysical Research Communications

Volumn 319, Issue 2, 2004, Pages 697-704

  Lombaerts, Marcel ^a   Middeldorp, Janneke W ^a   Van Der Weide, Esther ^a   Philippo, Katja ^a   Van Wezel, Tom ^a   Smit, Vincent T H B M ^a   Cornelisse, Cees J ^a   Cleton Jansen, Anne Marie ^a  

^a LEIDEN UNIVERSITY MEDICAL CENTER   (Netherlands)

Author keywords

Breast cancer; E cadherin; Infiltrating leukocytes; Methylation specific PCR; Promoter methylation

Indexed keywords

UVOMORULIN;

ARTICLE; BREAST TUMOR; CONTROLLED STUDY; ENERGY YIELD; EXPERIMENTAL TEST; FRAGMENTATION REACTION; GENE MUTATION; HUMAN; HUMAN CELL; HUMAN TISSUE; LEUKOCYTE MIGRATION; METHYLATION; MOLECULAR INTERACTION; MOLECULAR MECHANICS; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN DETERMINATION; PROTEIN EXPRESSION; SAMPLING; SEQUENCE ANALYSIS; T LYMPHOCYTE ACTIVATION; UVOMORULIN GENE;

BREAST NEOPLASMS; CADHERINS; DNA METHYLATION; HUMANS; LEUKOCYTES; POLYMERASE CHAIN REACTION; PROMOTER REGIONS (GENETICS);

EID: 2642552196     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bbrc.2004.05.041     Document Type: Article

References (25)

1. Vleminckx K., Vakaet L., Mareel M., Fiers W., van Roy F. Genetic manipulation of E-cadherin expression by epithelial tumor cells reveals an invasion suppressor role. Cell. 66:1991;107-119
2. Vleminckx K., Kemler R. Cadherins and tissue formation: integrating adhesion and signaling. Bioessays. 21:1999;211-220
3. Berx G., Becker K.F., Hofler H., van Roy F. Mutations of the human E-cadherin (CDH1) gene. Hum. Mutat. 12:1998;226-237
4. Siitonen S.M., Kononen J.T., Helin H.J., Rantala I.S., Holli K.A., Isola J.J. Reduced E-cadherin expression is associated with invasiveness and unfavorable prognosis in breast cancer. Am. J. Clin. Pathol. 105:1996;394-402
5. Cano A., Perez-Moreno M.A., Rodrigo I., Locascio A., Blanco M.J., del Barrio M.G., Portillo F., Nieto M.A. The transcription factor snail controls epithelial-mesenchymal transitions by repressing E-cadherin expression. Nat. Cell Biol. 2:2000;76-83
6. Comijn J., Berx G., Vermassen P., Verschueren K., van Grunsven L., Bruyneel E., Mareel M., Huylebroeck D., van Roy F. The two-handed E box binding zinc finger protein SIP1 downregulates E-cadherin and induces invasion. Mol. Cell. 7:2001;1267-1278
7. Hajra K.M., Chen D.Y., Fearon E.R. The SLUG zinc-finger protein represses E-cadherin in breast cancer. Cancer Res. 62:2002;1613-1618
8. Batlle E., Sancho E., Franci C., Dominguez D., Monfar M., Baulida J., Garcia D.H. The transcription factor snail is a repressor of E-cadherin gene expression in epithelial tumour cells. Nat. Cell Biol. 2:2000;84-89
9. Becker K.F., Atkinson M.J., Reich U., Becker I., Nekarda H., Siewert J.R., Hofler H. E-cadherin gene mutations provide clues to diffuse type gastric carcinomas. Cancer Res. 54:1994;3845-3852
10. Berx G., Cleton-Jansen A.M., Nollet F., De Leeuw W.J., Van de Vijver M.J., Cornelisse C., van Roy F. E-cadherin is a tumour/invasion suppressor gene mutated in human lobular breast cancers. EMBO J. 14:1995;6107-6115
11. Gayther S.A., Gorringe K.L., Ramus S.J., Huntsman D., Roviello F., Grehan N., Machado J.C., Pinto E., Seruca R., Halling K., MacLeod P., Powell S.M., Jackson C.E., Ponder B.A., Caldas C. Identification of germ-line E-cadherin mutations in gastric cancer families of European origin. Cancer Res. 58:1998;4086-4089
12. Guilford P., Hopkins J., Harraway J., McLeod M., McLeod N., Harawira P., Taite H., Scoular R., Miller A., Reeve A.E. E-cadherin germline mutations in familial gastric cancer. Nature. 392:1998;402-405
13. Graff J.R., Herman J.G., Lapidus R.G., Chopra H., Xu R., Jarrard D.F., Isaacs W.B., Pitha P.M., Davidson N.E., Baylin S.B. E-cadherin expression is silenced by DNA hypermethylation in human breast and prostate carcinomas. Cancer Res. 55:1995;5195-5199
14. Grady W.M., Willis J., Guilford P.J., Dunbier A.K., Toro T.T., Lynch H., Wiesner G., Ferguson K., Eng C., Park J.G., Kim S.J., Markowitz S. Methylation of the CDH1 promoter as the second genetic hit in hereditary diffuse gastric cancer. Nat. Genet. 26:2000;16-17
15. Tamura G., Yin J., Wang S., Fleisher A.S., Zou T., Abraham J.M., Kong D., Smolinski K.N., Wilson K.T., James S.P., Silverberg S.G., Nishizuka S., Terashima M., Motoyama T., Meltzer S.J. E-Cadherin gene promoter hypermethylation in primary human gastric carcinomas. J. Natl. Cancer Inst. 92:2000;569-573
16. Si H.X., Tsao S.W., Lam K.Y., Srivastava G., Liu Y., Wong Y.C., Shen Z.Y., Cheung A.L. E-cadherin expression is commonly downregulated by CpG island hypermethylation in esophageal carcinoma cells. Cancer Lett. 173:2001;71-78
17. Chang H.W., Chow V., Lam K.Y., Wei W.I., Yuen A. Loss of E-cadherin expression resulting from promoter hypermethylation in oral tongue carcinoma and its prognostic significance. Cancer. 94:2002;386-392
18. Fraga M.F., Esteller M. DNA methylation: a profile of methods and applications. Biotechniques. 33:2002;632. (see also pp. 634, 636-632, 634, 649)
19. Herman J.G., Graff J.R., Myohanen S., Nelkin B.D., Baylin S.B. Methylation-specific PCR: a novel PCR assay for methylation status of CpG islands. Proc. Natl. Acad. Sci. USA. 93:1996;9821-9826
20. De Leeuw W.J., Berx G., Vos C.B., Peterse J.L., Van de Vijver M.J., Litvinov S., van Roy F., Cornelisse C.J., Cleton-Jansen A.M. Simultaneous loss of E-cadherin and catenins in invasive lobular breast cancer and lobular carcinoma in situ. J. Pathol. 183:1997;404-411
21. Berx G., Cleton-Jansen A.M., Strumane K., De Leeuw W.J., Nollet F., van Roy F., Cornelisse C. E-cadherin is inactivated in a majority of invasive human lobular breast cancers by truncation mutations throughout its extracellular domain. Oncogene. 13:1996;1919-1925
22. Cleton-Jansen A.M., Moerland E.W., Kuipers-Dijkshoorn N.J., Callen D.F., Sutherland G.R., Hansen B., Devilee P., Cornelisse C.J. At least two different regions are involved in allelic imbalance on chromosome arm 16q in breast cancer. Genes Chromosomes Cancer. 9:1994;101-107
23. Bovee J.V., Devilee P., Cornelisse C.J., Schuuring E., Hogendoorn P.C. Identification of an EWS-pseudogene using translocation detection by RT-PCR in Ewing's sarcoma. Biochem. Biophys. Res. Commun. 213:1995;1051-1060
24. Ravenswaay Claasen H.H., Kluin P.M., Fleuren G.J. Tumor infiltrating cells in human cancer. On the possible role of CD16+ macrophages in antitumor cytotoxicity. Lab. Invest. 67:1992;166-174
25. Lehmann U., Celikkaya G., Hasemeier B., Langer F., Kreipe H. Promoter hypermethylation of the death-associated protein kinase gene in breast cancer is associated with the invasive lobular subtype. Cancer Res. 62:2002;6634-6638