Label-free impedance detection of oligonucleotide hybridisation on interdigitated ultramicroelectrodes using electrochemical redox probes

Biosensors and Bioelectronics

Volumn 21, Issue 4, 2005, Pages 645-654

  Dharuman, V ^a   Grunwald, T ^b   Nebling, E ^a   Albers, J ^a   Blohm, L ^b   Hintsche, R ^a,b  

^a FRAUNHOFER INSTITUTE FOR SILICON TECHNOLOGY ISIT   (Germany)

^b eBiochip Systems GmbH   (Germany)

Author keywords

Electrical biochips; IDA electrodes; Impedance; Label free detection; ODN hybridisation; Si technology

Indexed keywords

APPROXIMATION THEORY; CYCLIC VOLTAMMETRY; DIFFUSION; DNA; ELECTRIC IMPEDANCE; ELECTROCHEMISTRY; MICROELECTRODES; MONOLAYERS; REDOX REACTIONS; SPECTROSCOPIC ANALYSIS;

ELECTRICAL BIOCHIPS; IMPEDANCE; INTERDIGITATED ARRAY (IDA) ELECTRODES; LABEL-FREE DETECTION; OLIGODEOXYNUCLEOTIDE (ODN) HYBRIDIZATION; SI-TECHNOLOGY;

OLIGOMERS;

AMINOPHENOL DERIVATIVE; GOLD; IRON DERIVATIVE; METHYLENE BLUE; OLIGONUCLEOTIDE; POTASSIUM DERIVATIVE; SILICON; THIOL;

AMPEROMETRY; ARTICLE; COMPARATIVE STUDY; CYCLIC POTENTIOMETRY; DIFFUSION; DNA HYBRIDIZATION; DNA MICROARRAY; ELECTROCHEMICAL ANALYSIS; ELECTROCHEMICAL IMPEDANCE SPECTROSCOPY; ENZYME LINKED IMMUNOSORBENT ASSAY; IMMOBILIZATION; MEASUREMENT; MICROELECTRODE; OLIGONUCLEOTIDE PROBE; OXIDATION REDUCTION REACTION; SPECTROSCOPY; TECHNOLOGY;

DNA; ELECTRIC IMPEDANCE; ELECTROCHEMISTRY; EQUIPMENT DESIGN; EQUIPMENT FAILURE ANALYSIS; IN SITU HYBRIDIZATION; MICROELECTRODES; MINIATURIZATION; MOLECULAR PROBE TECHNIQUES; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS; OXIDATION-REDUCTION; STAINING AND LABELING;

EID: 25844457062     PISSN: 09565663     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bios.2004.12.020     Document Type: Article

References (37)

1. J. Albers, T. Grunwald, E. Nebling, G. Piechotta, and R. Hintsche Electrical biochip - a tool for microarrays and continuous monitoring Anal. Bioanal. Chem. 377 2003 521 527
2. E.M. Boon, D.M. Ceres, T.G. Drummond, M.G. Hill, and J.K. Barton Mutation detection by electrocatalysis at ODN modified electrodes Nat. Biotechnol. 18 2000 1096 1100
3. 2 substrates: characterisation by electrochemical impedance measurements and radiolabelling Biosens. Bioelectron. 17 2002 405 412
4. P. Diao, M. Guo, and R. Tung Characterisation of defects in the formation process of self-assembled thiol monolayers by electrochemical impedance spectroscopy J. Electroanal. Chem. 495 2001 98 105
5. R. Ehret, W. Baumann, M. Brischwein, A. Schwinde, K. Stegbauer, and B. Wolf Monitoring of cellular behavior by impedance measurements on interdigitated electrode structures Biosens. Bioelectron. 12 1997 29 41
6. H. Finklea, D.A. Sinder, J. Fedyk, E. Sabatani, Y. Gafni, and I. Rubinstein Characterisation of octadecanethiol coated gold electrodes as microarray electrodes by cyclic voltammetry and ac impedance spectroscopy Langmuir 9 1993 3660 3667 and references therein
7. G. Frace, G. Lillie, T. Hianik, P. Payne, and P. Vadgama Reagentless biosensing using electrochemical impedance spectroscopy Bioelectrochemitsry 55 2002 1 3
8. M. Gheorghe, and A.G. Elie Electrical frequency dependent characterisation of DNA hybridisation Biosens. Bioelectron. 19 2003 95 102
9. J.J. Gooding, A. Chou, F.J. Mearns, E. Wong, and K.L. Jericho The ion gating effect: using a change in flexibility to allow label free electrochemical detection of DNA hybridisation Chem. Commun. 2003 1938 1939
10. J.G. Guan, Y. Miao, and Q. Zhang Impedimetric biosensors J. Biosci. Bioeng. 97 2004 219 226
11. T.C. Hang, and A.G. Elie Frequency dependent and surface characterisation of DNA immobilisation and hybridisation Biosens. Bioelectron. 19 2004 1537 1548
12. R. Hintsche, B. Möller, I. Dransfeld, U. Wollenberger, F. Scheller, and B. Hoffmann Chip biosensors on thin-film metal electrodes Sens. Actuators B 4 1991 287 291
13. R. Hintsche, M. Paeschke, U. Wollenberger, U. Schnakenberg, B. Wagner, and T. Lisec Microelectrode arrays and application to biosensing devices Biosens. Bioelectron. 9 1994 697 705
14. E. Huang, M. Satjapipat, S. Han, and F. Zhou Surface structure and coverage of an oligonucleotide probe tethered onto gold substrate and its hybridisation efficiency for a polynucleotide target Langmuir 17 2001 1215 1224
15. J. Huangxian, C. Hongyuan, and G. Hong Investigation on microelectrodes. Part XVI. Study of the shielding effect at a microband-array electrode J. Electroanal. Chem. 341 1992 35 46
16. P. Jacobs Impedimetric detection of nucleic acid hybrids Proceedings of the Second International Conference on Microreaction Technology New Orleans, LA, USA, March 8.12 1998
17. E. Katz, and I. Willner Probing biomolecular interactions at conductive and semiconductive surfaces by impedance spectroscopy: routes to impedimetric immunosensors, DNA sensors, and enzyme biosensors Electroanalysis 15 2003 913 947 and references therein
18. S.O. Kelley, and J.K. Barton Electrochemistry of methylene blue bounded to a ODN modified electrode Bioconjugate Chem. 8 1997 31 37
19. W. Laureyn, D. Nelis, P. Van Gerwen, K. Baert, L. Hermans, R. Magnee, J.-J. Pireaux, and G. Maes Nanoscaled interdigitated titanium electrodes for impedimetric biosensing Sens. Actuators B68 2000 360 370
20. R.L. Levicky, T.M. Herne, M.J. Tarlov, and S.K. Satija Using self-assembly to control the structure of DNA monolayers on gold: a neutron reflectivity study J. Am. Chem. Soc. 120 1998 9787 9792
21. G. Lillie, P. Payne, and P. Vadgama Electrochemical impedance spectroscopy as a platform for reagentless bioaffinity sensing Sens. Actuators B 78 2001 249 256 and references therein
22. L. Montelius, J.O. Tegenfeldt, and T.G. Ling Fabrication and characterisation of a nanosensor for admittance spectroscopy of biomolecules J. Vac. Sci. Technol. A 13 1995 1755 1760
23. E. Nebling, T. Grunwald, J. Albers, J. Schäfer, and R. Hintsche Electrical detection of viral DNA using ultramicroelectrode arrays Anal. Chem. 76 2004 689 696
24. M. Paeschke, F. Dietrich, A. Uhlig, and R. Hintsche Voltammetric multichannel measurements using silicon fabricated microelectrode arrays Electroanalysis 8 1996 891 898
25. Paeschke, M., Buchmann, L.M., Seitz, R., Hintsche, R., 1996. Fabrication and investigation of nanometer sized dielectric integrated transducer for detection of biomolecular binding. In: Proceedings of the Microsystems Technologies, 96. VDE Verlag, p. 399 and the references therein.
26. N. Park, and J.H. Hahn Electrochemical sensing of DNA hybridisation based on duplex specific charge compensation Anal. Chem. 76 2004 900 906
27. A.W. Peterson, R.J. Heaton, and R.M. Georgiadis The effect of surface probe density on DNA hybridisation Nucl. Acids Res. 29 2001 5163 5168
28. L.V. Protsailo, and W.R. Fawcett Studies of electron transfer through self-assembled monolayers using impedance spectroscopy Electrochim. Acta 45 2000 3497 3505
29. E. Sabatani, and I. Rubinstein Organised self-assembling monolayers on electrodes. 2. Monolayer-based ultramicroelectrodes for the study of very rapid electrode kinetics J. Phys. Chem. 81 1987 6663 6669
30. A. Sadkowski, A.J. Motheo, and R.S. Neves Characterisation of Au(1 1 1) and Au(2 1 0) aqueous solution interfaces by electrochemical immittance spectroscopy J. Electroanal. Chem. 455 1998 107 119 and references therein
31. A.E.G. Saum, R.H. Cumming, and F.J. Rowell Use of substrate coated electrodes and AC impedance spectroscopy for the detection of enzyme activity Biosens. Bioelectron. 13 1998 511 518
32. E. Souteyrand, C. Chen, J.P. Cloarec, X. Nesme, P. Simonet, I. Navarro, and J.R. Martin Comparison between electrochemical and optoelectrochemical impedance measurements for the detection of DNA hybridisation Appl. Biochem. Biotechnol. 89 2000 195 207
33. A.B. Steel, T.M. Herne, and M.J. Tarlov Electrochemical quantification of DNA immobilised on gold Anal. Chem. 70 1998 4670 4677
34. K. Stulik, C. Amatore, K. Holub, V. Marecek, and W. Kutner Microelectrodes, definitions, characterisation and applications Pure Appl. Chem. 72 2000 1483 1492 and references therein
35. P.V. Van Gerwen, W. Laureyn, W. Laureys, H. Huyberechts, M. Op De Beeck, K. Baert, J. Suls, W. Sansen, P. Jacobs, L. Hermans, and R. Martens Nanoscaled interdigitated electrode arrays for biochemical sensors Sens. Actuators B 49 1998 73 80 and references therein
36. L. Yang, Y. Li, C.L. Griffis, and G.M. Johnson Interdigitated microelectrode (IME) impedance sensor for the detection of viable Salmonella typhimurium Biosens. Bioelectron. 19 2004 1139 1147
37. L. Yang, Y. Li, and G.F. Erf Interdigitated array microelectrode based electrochemical impedance immunosensor for detection of Escherichia coli 0157:H7 Anal. Chem. 2004 1107 1113