Cloning vectors and fluorescent proteins can significantly inhibit Salmonella enterica virulence in both epithelial cells and macrophages: Implications for bacterial pathogenesis studies

Infection and Immunity

Volumn 73, Issue 10, 2005, Pages 7027-7031

  Knodler, Leigh A ^a   Bestor, Aaron ^a   Ma, Caixia ^b   Hansen Wester, Imke ^c   Hensel, Michael ^c   Vallance, Bruce A ^b   Steele Mortimer, Olivia ^a,d  

^a NATIONAL INSTITUTES OF HEALTH   (United States)

^b UNIVERSITY OF BRITISH COLUMBIA   (Canada)

^c UNIVERSITY OF ERLANGEN NUREMBERG   (Germany)

^d NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

FLUORESCENT PROTEIN; GREEN FLUORESCENT PROTEIN; PLASMID VECTOR; PROTEIN; RED FLUORESCENT PROTEIN; UNCLASSIFIED DRUG;

ADOLESCENT; ANIMAL CELL; ANIMAL TISSUE; ARTICLE; BACTERIAL INFECTION; BACTERIAL VIRULENCE; CLONING VECTOR; EPITHELIUM CELL; FEMALE; FLUORESCENCE ANALYSIS; HUMAN; HUMAN CELL; INTERNALIZATION; MACROPHAGE; MAMMAL CELL; MOUSE; NONHUMAN; PRIORITY JOURNAL; REPORTER ASSAY; REPORTER GENE; SALMONELLA TYPHIMURIUM;

ANIMALS; CELLS, CULTURED; EPITHELIAL CELLS; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; HUMANS; MACROPHAGES; MICE; PHAGOCYTOSIS; PLASMIDS; SALMONELLA INFECTIONS; SALMONELLA TYPHIMURIUM; VIRULENCE;

EID: 25444469078     PISSN: 00199567     EISSN: None     Source Type: Journal    
DOI: 10.1128/IAI.73.10.7027-7031.2005     Document Type: Article

References (32)

1. Abromaitis, S., S. Faucher, M. Beland, R. Curtiss III, and F. Daigle. 2005. The presence of the tet gene from cloning vectors impairs Salmonella survival in macrophages. FEMS Microbiol. Lett. 242:305-312.
2. Alpuche-Aranda, C. M., E. L. Racoosin, J. A. Swanson, and S. I. Miller. 1994. Salmonella stimulate macrophage macropinocytosis and persist within spacious phagosomes. J. Exp. Med. 179:601-608.
3. Bolivar, F., R. L. Rodriguez, P. J. Greene, M. C. Betlach, H. L. Heyneker, and H. W. Boyer. 1977. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. Gene 2:95-113.
4. Bongaerts, R. J., I. Hautefort, J. M. Sidebotham, and J. C. Hinton. 2002. Green fluorescent protein as a marker for conditional gene expression in bacterial cells. Methods Enzymol. 358:43-66.
5. Buchmeier, N. A., and F. Heffron. 1989. Intracellular survival of wild-type Salmonella typhimurium and macrophage-sensitive mutants in diverse populations of macrophages. Infect. Immun. 57:1-7.
6. Bumann, D. 2002. Examination of Salmonella gene expression in an infected mammalian host using the green fluorescent protein and two-colour flow cytometry. Mol. Microbiol. 43:1269-1283.
7. Bumann, D. 2001. In vivo visualization of bacterial colonization, antigen expression, and specific T-cell induction following oral administration of live recombinant Salmonella enterica serovar Typhimurium. Infect. Immun. 69:4618-4626.
8. Chang, A. C. Y., and S. N. Cohen. 1978. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid. J. Bacteriol. 134:1141-1156.
9. Cirillo, D. M., R. H. Valdivia, D. M. Monack, and S. Falkow. 1998. Macrophage-dependent induction of the Salmonella pathogenicity island 2 type III secretion system and its role in intracellular survival. Mol. Microbiol. 30:175-188.
10. Fields, P. I., R. V. Swanson, C. G. Haidaris, and F. Heffron. 1986. Mutants of Salmonella typhimurium that cannot survive within the macrophage are avirulent. Proc. Natl. Acad. Sci. USA 83:5189-5193.
11. Galan, J. E. 2001. Salmonella interactions with host cells: type III secretion at work. Annu. Rev. Cell Dev. Biol. 17:53-86.
12. Galan, J. E., and D. Zhou. 2000. Striking a balance: modulation of the actin cytoskeleton by Salmonella. Proc. Natl. Acad. Sci. USA 97:8754-8761.
13. Gandhi, M., S. Golding, S. Yaron, and K. R. Matthews. 2001. Use of green fluorescent protein expressing Salmonella Stanley to investigate survival, spatial location, and control on alfalfa sprouts. J. Food Prot. 64:1891-1898.
14. Garmory, H. S., K. F. Griffin, S. E. Leary, S. D. Perkins, K. A. Brown, and R. W. Titball. 2002. The effect of recombinant plasmids on in vivo colonisation of Salmonella enterica serovar Typhimurium strains is not reflected by in vitro cellular invasion assays. Vaccine 20:3239-3243.
15. BAD promoter. J. Bacteriol. 177:4121-4130.
16. Hoiseth, S. K., and B. A. Stocker. 1981. Aromatic-dependent Salmonella typhimurium are non-virulent and effective as live vaccines. Nature 291:238-239.
17. Hopkins, S. A., F. Niedergang, I. E. Corthesy-Theulaz, and J. P. Kraehenbuhl. 2000. A recombinant Salmonella typhimurium vaccine strain is taken up and survives within murine Peyer's patch dendritic cells. Cell. Microbiol. 2:59-68.
18. Johansson, C., and M. J. Wick, 2004. Liver dendritic cells present bacterial antigens and produce cytokines upon Salmonella encounter. J. Immunol. 172:2496-2503.
19. Knodler, L. A., J. Celli, W. D. Hardt, B. A. Vallance, C. Yip, and B. B. Finlay. 2002. Salmonella effectors within a single pathogenicity island are differentially expressed and translocated by separate type III secretion systems. Mol. Microbiol. 43:1089-1103.
20. Knodler, L. A., B. A. Vallance, M. Hensel, D. Jackel, B. B. Finlay, and O. Steele-Mortimer. 2003. Salmonella type III effectors PipB and PipB2 are targeted to detergent-resistant microdomains on internal host cell membranes. Mol. Microbiol. 49:685-704.
21. Lee, A. K., C. S. Detweiler, and S. Falkow. 2000. OmpR regulates the two-component system SsrA-SsrB in Salmonella pathogenicity island 2. J. Bacteriol. 182:771-781.
22. Rang, C., J. E. Galen, J. B. Kaper, and L. Chao. 2003. Fitness cost of the green fluorescent protein in gastrointestinal bacteria. Can. J. Microbiol. 49:531-537.
23. Salcedo, S. P., M. Noursadeghi, J. Cohen, and D. W. Holden. 2001. Intracellular replication of Salmonella typhimurium strains in specific subsets of splenic macrophages in vivo. Cell. Microbiol. 3:587-597.
24. Sorensen, M., C. Lippuner, T. Kaiser, A. Misslitz, T. Aebischer, and D. Bumann. 2003. Rapidly maturing red fluorescent protein variants with strongly enhanced brightness in bacteria. FEBS Lett. 552:110-114.
25. Steele-Mortimer, O., S. Meresse, J. P. Gorvel, B. H. Toh, and B. B. Finlay. 1999. Biogenesis of Salmonella typhimurium-containing vacuoles in epithelial cells involves interactions with the early endocytic pathway. Cell. Microbiol. 1:33-49.
26. Swanson, J. A., and S. C. Baer. 1995. Phagocytosis by zippers and triggers. Trends Cell Biol. 5:89-93.
27. Valdivia, R. H., and S. Falkow. 1996. Bacterial genetics by flow cytometry: rapid isolation of Salmonella typhimurium acid-inducible promoters by differential fluorescence induction. Mol. Microbiol. 22:367-378.
28. Valdivia, R. H., and S. Falkow. 1997. Fluorescence-based isolation of bacterial genes expressed within host cells. Science 277:2007-2011.
29. Valdivia, R. H., A. E. Hromockyj, D. Monack, L. Ramakrishnan, and S. Falkow. 1996. Applications for green fluorescent protein (GFP) in the study of host-pathogen interactions. Gene 173:47-52.
30. Valdivia, R. H., and L. Ramakrishnan. 2000. Applications of gene fusions to green fluorescent protein and flow cytometry to the study of bacterial gene expression in host cells. Methods Enzymol. 326:47-73.
31. Wang, R. F., and S. R. Kushner. 1991. Construction of versatile low-copy-number vectors for cloning, sequencing and gene expression in Escherichia coli. Gene 100:195-199.
32. Yrlid, U., and M. J. Wick. 2002. Antigen presentation capacity and cytokine production by murine splenic dendritic cell subsets upon Salmonella encounter. J. Immunol. 169:108-116.