Structure-based design of Taq DNA polymerases with improved properties of dideoxynucleotide incorporation

Proceedings of the National Academy of Sciences of the United States of America

Volumn 96, Issue 17, 1999, Pages 9491-9496

  Li, Ying ^a   Mitaxov, Vesselin ^a   Waksman, Gabriel ^a  

^a WASHINGTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

DNA sequencing; X ray crystallography

Indexed keywords

DNA POLYMERASE;

ARTICLE; CRYSTAL STRUCTURE; CRYSTALLIZATION; DNA SEQUENCE; ENZYME ACTIVITY; ENZYME STRUCTURE; MOLECULAR WEIGHT; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS; STATISTICAL ANALYSIS; X RAY CRYSTALLOGRAPHY;

CRYSTALLOGRAPHY, X-RAY; DEOXYADENINE NUCLEOTIDES; DEOXYCYTOSINE NUCLEOTIDES; DEOXYGUANINE NUCLEOTIDES; DEOXYRIBONUCLEOTIDES; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PROTEIN CONFORMATION; PROTEIN STRUCTURE, SECONDARY; TAQ POLYMERASE; THYMINE NUCLEOTIDES;

EID: 0033578332     PISSN: 00278424     EISSN: None     Source Type: Journal    
DOI: 10.1073/pnas.96.17.9491     Document Type: Article

References (9)

1. Saiki, R. K., Gelfand, D. H., Stoffel, S., Scharf, S. J., Higuchi, R., Horn, G. T., Mullis, K. B. & Erlich, H. A. (1988) Science 239, 487-491.
2. Innis, M. A., Myambo, K. B., Gelfand, D. H. & Brow, M. A. D. (1988) Proc. Natl. Acad. Sci. USA 85, 9436-9440.
3. Lee, L. G., Connell, C. R., Woo, S. L., Cheng, R. D., McArdie, B. F., Fuller, C. W., Halloran, N. D. & Wilson, R. K. (1992) Nucleic Acids Res. 20, 2471-2483.
4. Brandis, J. W., Edwards, S. G. & Johnson, K. A. (1996) Biochemistry 35, 2189-2200.
5. Tabor, S. & Richardson, C. C. (1995) Proc. Natl. Acad. Sci. USA 92, 6339-6343.
6. Li, Y., Korolev, S. V. & Waksman, G. (1998) EMBO J. 17, 7514-7525.
7. Brünger, A. T., Adams, P. D., Clore, G. M., DeLano, W. L., Gros, P., Grosse-Kunstleve, R. W., Jiang, J. S., Kuszewski, J., Nilges, M., Pannu, N. S., et al. (1998) Acta Crystallogr. D 54, 905-921.
8. Hodel, A., Kim, S.-H. & Brünger, A. T. (1992) Acta Crystallogr. A 48, 851-858.
9. Fuller, C. W. (1992) Methods Enzymol. 216, 329-354.