Active nuclear import and export is independent of lumenal Ca2+ stores in intact mammalian cells

Journal of General Physiology

Volumn 113, Issue 2, 1999, Pages 239-248

  Strübing, Garsten ^a   Clapham, David E ^b  

^a HOWARD HUGHES MEDICAL INSTITUTE   (United States)

^b BOSTON CHILDREN S HOSPITAL   (United States)

Author keywords

Ca2+ store depletion; Green fluorescent protein; Nuclear pore complex; Nuclear transport

Indexed keywords

CALCIUM; CARBACHOL; GLUCOCORTICOID RECEPTOR; GREEN FLUORESCENT PROTEIN; IONOMYCIN; MITOGEN ACTIVATED PROTEIN KINASE; MUSCARINIC M1 RECEPTOR; NUCLEAR PROTEIN; THAPSIGARGIN;

ACTIVE TRANSPORT; ARTICLE; CALCIUM CELL LEVEL; CALCIUM TRANSPORT; CELL MEMBRANE PERMEABILITY; CELL NUCLEUS; CELL TRANSPORT; CYTOPLASM; DIFFUSION; HUMAN; HUMAN CELL; INHIBITION KINETICS; PROTEIN DEPLETION; PROTEIN TRANSPORT; REGULATORY MECHANISM;

EID: 2542508417     PISSN: 00221295     EISSN: None     Source Type: Journal    
DOI: 10.1085/jgp.113.2.239     Document Type: Article

References (40)

1. 2+ depletion blocks endosome fusion. Biochem. J. 312:919-923.
2. 2+. J. Biol. Chem. 260:2719-2727.
3. Berridge, M.J. 1993. Inositol trisphosphate and calcium signalling. Nature. 361:315-325.
4. Bonner, W.M. 1975. Protein migration into nuclei. II. Frog oocyte nuclei accumulate a class of microinjected oocyte nuclear proteins and exclude a class of microinjected oocyte cytoplasmic proteins. J. Cell Biol. 64:431-437.
5. Carey, K.L., S.A. Richards, K.M. Lounsbury, and I.G. Macara. 1996. Evidence using a green fluorescent protein-glucocorticoid receptor chimera that the Ran/TC4 GTPase mediates an essential function independent of nuclear protein import. J. Cell Biol. 133: 985-996.
6. Csermely, P., P. Sandor, L. Radics, and J. Somogyi. 1989. Zinc forms complexes with higher kinetical stability than calcium, 5-F-BAPTA as a good example. Biochem. Biophys. Res. Commun. 165:838-844.
7. Engel, K., A. Kotlyarov, and M. Gaestel. 1998. Leptomycin B-sensitive nuclear export of MAPKAP kinase 2 is regulated by phosphorylation. EMBO (Eur. Mol. Biol Organ.) J. 17:3363-3371.
8. Feldherr, C.M., E. Kallenbach, and A.N. Schultz. 1984. Movement of a karyophilic protein through the nuclear pores of oocytes. J. Cell Biol. 99:2216-2222.
9. Freshney, N.W., L. Rawlinson, F. Guesdon, E. Jones, S. Cowley, J. Hsuan, and J. Saklatvala. 1994. Interleukin-1 activates a novel protein kinase cascade that results in the phosphorylation of Hsp27. Cell 78:1039-1049.
10. Görlich, D. 1997. Nuclear protein import. Curr. Opin. Cell Biol. 9:412-419.
11. Görlich, D. 1998. Transport into and out of the cell nucleus. EMBO (Eur: Mol. Biol. Organ.) J. 17:2721-2727.
12. Greber, U.F., and L. Gerace. 1992. Nuclear protein import is inhibited by an antibody to a lumenal epitope of a nuclear pore complex glycoprotein. J. Cell Biol. 116:15-30.
13. Greber, U.F., and L. Gerace. 1995. Depletion of calcium from the lumen of endoplasmic reticulum reversibly inhibits passive diffusion and signal-mediated transport into the nucleus. J. Cell Biol. 128:5-14.
14. Greber, U.F., M. Suomalainen, R.P. Stidwill, K. Boucke, M.W. Ebersold, and A. Helenius. 1997. The role of the nuclear pore complex in adenovirus DNA entry. EMBO (Eur. Mol. Biol. Organ.) J. 16:5998-6007.
15. 2+]. J. Cell Biol. 140:325-334.
16. Htun, H., H. Barsony, I. Renyi, D.L. Gould, and G.L. Hager. 1996. Visualization of glucocorticoid receptor translocation and intranuclear organization in living cells with a green fluorescent protein chimera. Proc. Natl. Acad. Sci. USA. 93:4845-4850.
17. 2 hydrolysis and regulation of phospholipase C isozymes. Curr. Opin. Cell. Biol. 7:183-189.
18. Liu, C., and T.E. Hermann. 1978. Characterization of ionomycin as a calcium ionophore. J. Biol. Chem. 253:5892-5894.
19. Love, D.C., T.D. Sweitzer, and J.A. Hanover. 1998. Reconstitution of HIV-1 Rev nuclear export: independent requirements for nuclear import and export. Proc. Natl. Acad. Sci. USA. 95:10608-10613.
20. 2+ stores are not required for nuclear envelope assembly or nuclear protein import in Xenopus egg extracts. Cell Calc. 21:151-161.
21. Menezes, A., R. Zeman, and E.-H. Sabban. 1996. Involvement of intracellular or extracellular calcium in activation of tyrosine hydroxylase gene expression in PCI2 cells. J. Neurochem. 67:2316-2324.
22. Nigg, E.A. 1997. Nucleocytoplasmic transport: signals, mechanisms and regulation. Nature. 386:779-787.
23. Paine, P.L., L.C. Moore, and S.B. Horowitz. 1975. Nuclear envelope permeability. Nature. 254:109-114.
24. Peralta, E.G., A. Ashkenazi, J.W. Winslow, J. Ramachandran, and D.J. Capon. 1988. Differential regulation of PI hydrolysis and adenylyl cyclase by muscarinic receptor subtypes. Mature. 334: 434-437.
25. 2+ stores. Science. 273: 1875-1877.
26. 2+. Cell Calc. 22:209-216.
27. Rouse, J., P. Cohen, S. Trigon, M. Morange, A. Alonso-Llamazares, D. Zamanillo, T. Hunt, and A.R Nebreda. 1994. A novel kinase cascade triggered by stress and heat shock that stimulates MAP-KAP kinase-2 and phosphorylation of the small heat shock proteins. Cell. 78:1027-1037.
28. Sambrook, J., E.F. Fritsch, and T. Maniatis. 1989. Molecular Cloning: A Laboratory Manual. 2nd ed. Cold Spring Harbor Laboratory Press, Plainview, NY. 16.30-16.37.
29. Schubert, R. 1990. A program for calculating multiple metal-ligand solutions. Compute. Methods Programs Biomed. 33:93-94.
30. 2+, inhibits lamin assembly, destabilizes nuclear architecture and may independently protect nuclei from apoptosis in vitro. Cell Calc. 23:151-164.
31. 2+ store. Science. 270:1835-1838.
32. 3 receptor channels. Neuron. 14: 163-167.
33. Sweitzer, T.D., and J.A. Hanover. 1996. Calmodulin activates nuclear protein import: a link between signal transduction and nuclear transport. Proc. Natl. Acad. Sci. USA. 93:14574-14579.
34. 2+-ATPase. Proc. Natl. Acad. Sci. USA. 87:2466-2470.
35. Tsien, R.Y. 1980. New calcium indicators and buffers with high selectivity against magnesium and protons: design, synthesis, and properties of prototype structures. Biochemistry. 19:2396-3404.
36. Tsien, R.Y., T. Pozzan, and T.J. Rink. 1984. Calcium activities and fluxes inside small intact cells as measured with intracellularly trapped chelators. Adv. Cyclic Nucleotide Protein Phosphorylation Res. 17:535-541.
37. Ullman, K., M. Powers, and D. Forbes. 1997. Nuclear export receptors: from importin to exportin. Cell. 90:967-970.
38. Ward, W.W., H.J. Prentice, A.F. Roth, C.W. Cody, and S.C. Reeves. 1982. Spectral pertubations of the Aequorea green-fluorescent protein. Photochem. Photobiol. 35:803-808.
39. Waser, M., N. Mesaeli, C. Spencer, and M. Michalak. 1997. Regulation of calreticulin gene expression by calcium. J. Cell Biol. 138: 547-557.
40. Weiss, C. 1998. Importins and exporting: how to get in and out of the nucleus. TIBS (Trends Biochem. Sci.). 23:185-189.