A fast and flexible PEG-mediated transient expression system in plants for high level expression of secreted recombinant proteins

Journal of Biotechnology

Volumn 119, Issue 4, 2005, Pages 332-342

  Baur, Armin ^a,b   Kaufmann, Franz ^a   Rolli, Helene ^a   Weise, Andreas ^a   Luethje, Rasmus ^a   Berg, Birgit ^a   Braun, Michael ^c   Baeumer, Wolfgang ^c   Kietzmann, Manfred ^c   Reski, Ralf ^b   Gorr, Gilbert ^a  

^a Greenovation Biotech GmbH   (Germany)

^b UNIVERSITY OF FREIBURG   (Germany)

^c UNIVERSITY OF VETERINARY MEDICINE HANNOVER   (Germany)

Author keywords

PEG mediated transformation; Physcomitrella patens; Recombinant human vascular endothelial growth factor; Signal peptide; Transient expression

Indexed keywords

DRUG PRODUCTS; PLANTS (BOTANY); POLYPEPTIDES; PROTEINS;

PED-MEDIATED TRANSFORMATIONS; PHYSCOMETRELLA PATENS; RECOMBINANT HUMAN VASCULAR ENDOTHELIAL GROWTH FACTOR; SIGNAL PEPTIDES; TRANSIENT EXPRESSION;

POLYETHYLENE GLYCOLS;

GLYCAN DERIVATIVE; MACROGOL; RECOMBINANT PROTEIN; RECOMBINANT VASCULOTROPIN; SIGNAL PEPTIDE; SUGAR; UNCLASSIFIED DRUG; VASCULOTROPIN; VASCULOTROPIN 121; VEGETABLE PROTEIN;

ARTICLE; BIOLOGICAL ACTIVITY; CULTURE MEDIUM; GENE CONSTRUCT; GENE EXPRESSION SYSTEM; HUMAN; HUMAN CELL; IMMUNOGENICITY; MOSS; NONHUMAN; NUCLEOTIDE SEQUENCE; PLANT GENETICS; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN ANALYSIS; PROTEIN ENGINEERING; PROTEIN EXPRESSION; PROTEIN PROCESSING; PROTEIN SECRETION; PROTOPLAST; SILVER STAINING; WESTERN BLOTTING;

BRYOPSIDA; PLANTS, GENETICALLY MODIFIED; POLYETHYLENE GLYCOLS; PROTEIN ENGINEERING; PROTOPLASTS; RECOMBINANT PROTEINS; TRANSFECTION; VASCULAR ENDOTHELIAL GROWTH FACTOR A;

BRYOPHYTA; PHYSCOMITRELLA PATENS;

EID: 24944531347     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2005.04.018     Document Type: Article

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