RNAi-mediated silencing of leptin gene expression increases cell death in C6 glioblastoma cells

Molecular Brain Research

Volumn 139, Issue 2, 2005, Pages 357-360

  Brown, Russell ^a   Morash, Barbara ^a   Ur, Ehud ^a   Wilkinson, Michael ^a,b  

^a DALHOUSIE UNIVERSITY   (Canada)

^b DALHOUSIE UNIVERSITY   (Canada)

Author keywords

Brain; Cell death; Leptin; Reverse transcription polymerase chain reaction; RNA interference; Small interfering RNA

Indexed keywords

LEPTIN; MESSENGER RNA; SMALL INTERFERING RNA;

ANIMAL CELL; ARTICLE; CELL DEATH; CONTROLLED STUDY; GENE EXPRESSION; GENE SILENCING; GENETIC TRANSFECTION; GLIOMA; IN VITRO STUDY; NICK END LABELING; NONHUMAN; PRIORITY JOURNAL; PROTEIN FUNCTION; RAT; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA INTERFERENCE;

ANALYSIS OF VARIANCE; ANIMALS; CELL COUNT; CELL DEATH; CELL LINE, TUMOR; DOSE-RESPONSE RELATIONSHIP, DRUG; GENE EXPRESSION REGULATION; GENE SILENCING; GLIOBLASTOMA; LEPTIN; RATS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RIBOSOMAL PROTEINS; RNA INTERFERENCE; RNA, MESSENGER; RNA, NEOPLASM; RNA, SMALL INTERFERING; TIME FACTORS;

EID: 24944523270     PISSN: 0169328X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.molbrainres.2005.05.009     Document Type: Article

References (28)

1. R.S. Ahima, and J.S. Flier Leptin Annu. Rev. Physiol. 62 2000 413 437
2. R.S. Ahima, C. Bjorbaek, S. Osei, and J.S. Flier Regulation of neuronal and glial proteins by leptin: implications for brain development Endocrinology 140 1999 2755 2762
3. S.G. Bouret, S.J. Draper, and R.B. Simerly Trophic action of leptin on hypothalamic neurons that regulate feeding Science 304 2004 108 110
4. D.A. Braasch, S. Jensen, Y. Liu, K. Kaur, K. Arar, M.A. White, and D.R. Corey RNA interference in mammalian cells by chemically-modified RNA Biochemistry 42 2003 7967 7975
5. J. Couzin Molecular biology. RNAi shows cracks in its armor Science 306 2004 1124 1125
6. E. Dicou, S. Attoub, and P. Gressens Neuroprotective effects of leptin in vivo and in vitro NeuroReport 12 2001 3947 3951
7. G. Dorn, S. Patel, G. Wotherspoon, M. Hemmings-Mieszczak, J. Barclay, F.J. Natt, P. Martin, S. Bevan, A. Fox, P. Ganju, W. Wishart, and J. Hall siRNA relieves chronic neuropathic pain Nucleic Acids Res. 32 2004 e49
8. R.A. Ehrhardt, A.W. Bell, and Y.R. Boisclair Spatial and developmental regulation of leptin in fetal sheep Am. J. Physiol.: Regul., Integr. Comp. Physiol. 282 2002 R1628 R1635
9. S.M. Elbashir, J. Harborth, K. Weber, and T. Tuschl Analysis of gene function in somatic mammalian cells using small interfering RNAs Methods 26 2002 199 213
10. J.K. Elmquist, C. Bjorbaek, R.S. Ahima, J.S. Flier, and C.B. Saper Distributions of leptin receptor mRNA isoforms in the rat brain J. Comp. Neurol. 395 1998 535 547
11. M.L. Hakansson, H. Brown, N. Ghilardi, R.C. Skoda, and B. Meister Leptin receptor immunoreactivity in chemically defined target neurons of the hypothalamus J. Neurosci. 18 1998 559 572
12. J.D. Hommel, R.M. Sears, D. Georgescu, D.L. Simmons, and R.J. DiLeone Local gene knockdown in the brain using viral-mediated RNA interference Nat. Med. 9 2003 1539 1544
13. M.J. Ishimaru, C. Ikonomidou, T.I. Tenkova, T.C. Der, K. Dikranian, M.A. Sesma, and J.W. Olney Distinguishing excitotoxic from apoptotic neurodegeneration in the developing rat brain J. Comp. Neurol. 408 1999 461 476
14. A.L. Jackson, and P.S. Linsley Noise amidst the silence: off-target effects of siRNAs? Trends Genet. 20 2004 521 524
15. L. Jin, S. Zhang, B.G. Burguera, M.E. Couce, R.Y. Osamura, E. Kulig, and R.V. Lloyd Leptin and leptin receptor expression in rat and mouse pituitary cells Endocrinology 141 2000 333 339
16. A. Levesque, A. Paquet, and M. Page Improved fluorescent bioassay for the detection of tumor necrosis factor activity J. Immunol. Methods 178 1995 71 76
17. Y.J. Ma, D.F. Hill, M.P. Junier, M.E. Costa, S.E. Felder, and S.R. Ojeda Expression of epidermal growth factor receptor changes in the hypothalamus during the onset of female puberty Mol. Cell. Neurosci. 5 1994 246 262
18. H. Makimura, T.M. Mizuno, J.W. Mastaitis, R. Agami, and C.V. Mobbs Reducing hypothalamic AGRP by RNA interference increases metabolic rate and decreases body weight without influencing food intake BMC Neurosci. 3 2002 18
19. B. Morash, A. Li, P.R. Murphy, M. Wilkinson, and E. Ur Leptin gene expression in the brain and pituitary gland Endocrinology 140 1999 5995 5998
20. B. Morash, J. Johnstone, C. Leopold, A. Li, P. Murphy, E. Ur, and M. Wilkinson The regulation of leptin gene expression in the C6 glioblastoma cell line Mol. Cell. Endocrinol. 165 2000 97 105
21. V.C. Russo, S. Metaxas, K. Kobayashi, M. Harris, and G.A. Werther Antiapoptotic effects of leptin in human neuroblastoma cells Endocrinology 145 2004 4103 4112
22. A. Sahu Minireview: a hypothalamic role in energy balance with special emphasis on leptin Endocrinology 145 2004 2613 2620
23. D. Semizarov, L. Frost, A. Sarthy, P. Kroeger, D.N. Halbert, and S.W. Fesik Specificity of short interfering RNA determined through gene expression signatures Proc. Natl. Acad. Sci. U. S. A. 100 2003 6347 6352
24. N. Smolinska, J. Przala, T. Kaminski, G. Siawrys, A. Gajewska, K. Kochman, and S. Okrasa Leptin gene expression in the hypothalamus and pituitary of pregnant pigs Neuro-Endocrinol. Lett. 25 2004 191 195
25. A. Spanova, H. Kovaru, V. Lisa, E. Lukasova, and B. Rittich Estimation of apoptosis in C6 glioma cells treated with antidepressants Physiol. Res. 46 1997 161 164
26. C.M. Steppan, and A.G. Swick A role for leptin in brain development Biochem. Biophys. Res. Commun. 256 1999 600 602
27. E. Ur, D.A. Wilkinson, B.A. Morash, and M. Wilkinson Leptin immunoreactivity is localized to neurons in rat brain Neuroendocrinology 75 2002 264 272
28. M. Wilkinson, B. Morash, and E. Ur The brain is a source of leptin Front. Horm. Res. 26 2000 106 125