Molecular biology: A strategy for probing the function of noncoding RNAs finds a repressor of NFAT

Science

Volumn 309, Issue 5740, 2005, Pages 1570-1573

  Willingham, A T ^a   Orth, A P ^b   Batalov, S ^b   Peters, E C ^b   Wen, B G ^b   Aza Blanc, P ^b   Hogenesch, J B ^b,c   Schultz, P G ^a,b  

^a SCRIPPS RESEARCH INSTITUTE   (United States)

^b GENOMICS INSTITUTE OF THE NOVARTIS RESEARCH FOUNDATION   (United States)

^c SCRIPPS RESEARCH INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOASSAY; GENES; MOLECULAR BIOLOGY; PROTEINS;

BIOLOGICAL PROCESSES; CELL-BASED ASSAYS; FUNCTIONAL ANALYSIS; TRANSCRIPTIONAL REGULATION;

RNA;

CALCINEURIN INHIBITOR; COMPLEMENTARY DNA; CYCLOSPORIN A; RNA HELICASE; SHORT HAIRPIN RNA; TRANSCRIPTION FACTOR NFAT; UNTRANSLATED RNA; DNA BINDING PROTEIN; KARYOPHERIN BETA; NRON RNA, MOUSE; NUCLEAR PROTEIN; TRANSCRIPTION FACTOR;

RNA;

AMINO ACID SEQUENCE; ANIMAL CELL; ARTICLE; CELL STRAIN 3T3; CONTROLLED STUDY; EMBRYO; GENE EXPRESSION REGULATION; GENE STRUCTURE; GENETIC TRANSCRIPTION; HUMAN; HUMAN CELL; HUMAN GENOME; KIDNEY CELL; LEUKEMIA CELL LINE; MASS SPECTROMETRY; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN RNA BINDING; PROTEIN SYNTHESIS; RNA INTERFERENCE; RNA SEQUENCE; SIGNAL TRANSDUCTION; ANIMAL; CELL LINE; DRUG ANTAGONISM; GENETICS; METABOLISM; PHYSIOLOGY;

ANIMALIA;

ANIMALS; BETA KARYOPHERINS; CELL LINE; DNA-BINDING PROTEINS; HUMANS; MICE; NFATC TRANSCRIPTION FACTORS; NUCLEAR PROTEINS; RNA INTERFERENCE; RNA, UNTRANSLATED; TRANSCRIPTION FACTORS;

EID: 24644481406     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.1115901     Document Type: Article

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25. We thank L. Miraglia, S. Chanda, S. White, C. Cooper, M. Hancock, J. Liu, and Q. Huang for their assistance in cell-based assays and screenings; J. Walker for sharing RNAs from mouse and human tissues; J. Grbic for help with experiments; L. Linford, M. Medina, and B. Smith for help preparing the shRNA library; and P. DeJesus and J. Graziano for aid with proteomics efforts. The NFATc1-GFP construct was a generous gift from L. Gerace, as was FOXO1-GFP provided by W. Sellers. This work was supported by the Novartis Research Foundation and an NIH Kirschstein National Research Service Award for A.T.W. This is manuscript 17361-CH of the Scripps Research Institute and is dedicated to Peter B. Dervan on the occasion of his 60th birthday.