Mass spectrometry of hydrogen/deuterium exchange of Escherichia coli dihydrofolate reductase: Effects of loop mutations

Journal of Biochemistry

Volumn 135, Issue 4, 2004, Pages 487-494

  Yamamoto, Tatsuya ^a   Izumi, Shunsuke ^a   Ohmae, Eiji ^a   Gekko, Kunihiko ^a  

^a HIROSHIMA UNIVERSITY   (Japan)

Author keywords

Dihydrofolate reductase; H D exchange; Loop mutation; Mass spectrometry; Structural fluctuation

Indexed keywords

DEUTERIUM; DIHYDROFOLATE REDUCTASE; GLYCINE; MUTANT PROTEIN;

AMINO ACID SUBSTITUTION; ARTICLE; ENZYME ACTIVITY; ENZYME KINETICS; ENZYME LOCALIZATION; ENZYME STABILITY; ENZYME STRUCTURE; ESCHERICHIA COLI; MASS SPECTROMETRY; MUTATION; NONHUMAN; PROTON TRANSPORT; WILD TYPE;

ALGORITHMS; AMINO ACIDS; DEUTERIUM EXCHANGE MEASUREMENT; DEUTERIUM OXIDE; ESCHERICHIA COLI; KINETICS; MOLECULAR WEIGHT; MUTAGENESIS, SITE-DIRECTED; POINT MUTATION; RECOMBINANT PROTEINS; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; TETRAHYDROFOLATE DEHYDROGENASE; THERMODYNAMICS;

ESCHERICHIA COLI; NEGIBACTERIA;

EID: 2442680075     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/jb/mvh056     Document Type: Article

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