A highly active synthetic mammalian retrotransposon

Nature

Volumn 429, Issue 6989, 2004, Pages 314-318

  Han, Jeffrey S ^a   Boeke, Jef D ^a  

^a JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; ASSAYS; ENZYMES; GENES; RNA;

CODING REGIONS; RETOTRANSPOSITION; ROBUST EXPRESSION;

GENETIC ENGINEERING;

AMINO ACID; OLIGONUCLEOTIDE; PROTEIN; RNA;

BIOLOGY; MAMMAL;

AMINO ACID SEQUENCE; ANIMAL CELL; ARTICLE; CONTROLLED STUDY; GENOME; HUMAN; HUMAN CELL; IMMUNOBLOTTING; MOUSE; NONHUMAN; NORTHERN BLOTTING; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN EXPRESSION; RETROPOSON; ANIMAL; CELL STRAIN 3T3; GENETIC ENGINEERING; GENETIC RECOMBINATION; GENETIC TRANSCRIPTION; GENETICS; HELA CELL; LONG INTERSPERSED REPEAT; MUTAGENESIS; PROTEIN SYNTHESIS;

ANIMALIA; MAMMALIA;

3T3 CELLS; ANIMALS; BASE SEQUENCE; GENETIC ENGINEERING; HELA CELLS; HUMANS; LONG INTERSPERSED NUCLEOTIDE ELEMENTS; MICE; MUTAGENESIS; OPEN READING FRAMES; PROTEIN BIOSYNTHESIS; PROTEINS; RECOMBINATION, GENETIC; TRANSCRIPTION, GENETIC;

EID: 2442671716     PISSN: 00280836     EISSN: None     Source Type: Journal    
DOI: 10.1038/nature02535     Document Type: Article

References (23)

1. Lander, E. S. et al. Initial sequencing and analysis of the human genome. Nature 409, 860-921 (2001).
2. Han, J. S., Szak, S. T. & Boeke, J. D. Transcriptional disruption by the L1 retrotransposon and implications for mammalian transcriptomes. Nature 429, 268-274 (2004).
3. Feng, Q., Moran, J. V., Kazazian, H. H. Jr & Boeke, J. D. Human L1 retrotransposon encodes a conserved endonuclease required for retrotransposition. Cell 87, 905-916 (1996).
4. Moran, J. V. et al. High frequency retrotransposition in cultured mammalian cells. Cell 87, 917-927 (1996).
5. Cost, G. J., Feng, Q., Jacquier, A. & Boeke, J. D. Human L1 element target-primed reverse transcription in vitro. EMBO J. 21, 5899-5910 (2002).
6. Dewannieux, M., Esnault, C. & Heidmann, T. LINE-mediated retrotransposition of marked Alu sequences. Nature Genet. 35, 41-48 (2003).
7. Martin, S. L. Characterization of a LINE-1 cDNA that originated from RNA present in the ribonucleoprotein particles: implications for the structure of an active mouse LINE-1. Gene 153, 261-266 (1995).
8. Kolosha, V. O. & Martin, S. L. In vitro properties of the first ORF protein from mouse LINE-1 support its role in ribonucleoprotein particle formation during retrotransposition. Proc. Natl. Acad. Sci. USA 94, 10155-10160 (1997).
9. Esnault, C., Maestre, J. & Heidmann, T. Human LINE retrotransposons generate processed pseudogenes. Nature Genet. 24, 363-367 (2000).
10. Wei, W. et al. Human L1 retrotransposition: cis preference versus trans complementation. Mol. Cell. Biol. 21, 1429-1439 (2001).
11. Mathias, S. L., Scott, A. F., Kazazian, H. H. Jr, Boeke, J. D. & Gabriel, A. Reverse transcriptase encoded by a human transposable element. Science 254, 1808-1810 (1991).
12. Stemmer, W. P., Crameri, A., Ha, K. D., Brennan, T. M. & Heyneker, H. L. Single-step assembly of a gene and entire plasmid from large numbers of oligodeoxyribonucleotides. Gene 164, 49-53 (1995).
13. Boissinot, S., Chevret, P. & Furano, A. V. L1 (LINE-1) retrotransposon evolution and amplification in recent human history. Mol. Biol. Evol. 17, 915-928 (2000).
14. Gilbert, N., Lutz-Prigge, S. & Moran, J. V. Genomic deletions created upon LINE-1 retrotransposition. Cell 110, 315-325 (2002).
15. Symer, D. E. et al. Human L1 retrotransposition is associated with genetic instability in vivo. Cell 110, 327-338 (2002).
16. Cost, G. J. & Boeke, J. D. Targeting of human retrotransposition integration is directed by the specificity of the L1 endonuclease for regions of unusual DNA structure. Biochemistry 37, 18081-18093 (1998).
17. Wei, W., Morrish, T. A., Alfsch, R. S. & Moran, J. V. A transient assay reveals that cultured human cells can accommodate multiple LINE-1 retrotransposition events. Anal. Biochem. 284, 435-438 (2000).
18. Moran, I. V., DeBerardinis, R. J. & Kazazian, H. H. Jr Exon shuffling by L1 retrotransposition. Science 283, 1530-1534 (1999).
19. Hamer, L., DeZwaan, T. M., Montenegro-Chamorro, M. V., Frank, S. A. & Hamer, J. E. Recent advances in large-scale transposon mutagenesis. Curr. Opin. Chem. Biol. 5, 67-72 (2001).
20. Ostertag, E. M. et al. A mouse model of human L1 retrotransposition. Nature Genet. 32, 655-660 (2002).
21. Haas, J., Park, E.-C. & Seed, B. Codon usage limitation in the expression of HIV-1 envelope glycoprotein. Curr. Biol. 6, 315-324 (1996).
22. Naas, T. P. et al. An actively retrotransposing, novel subfamily of mouse L1 elements. EMBO J. 17, 590-597 (1998).
23. Morrish, T. A. et al. DNA repair mediated by endonuclease-independent LINE-1 retrotransposition. Nature Genet. 31, 159-165 (2002).