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This geometry (1.23 Å distance and 110° angle) is incompatible with the binding of azide. Azide was used in the crystallization conditions described here as well as elsewhere (15, 16) and has not been observed bound to either of the active-site copper atoms.
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18O); however, this complex has eluded structural study (42).
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2442446599
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note
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The figures in this manuscript were made with the program SETOR (43).
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45
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2442599578
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note
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Coordinates have been deposited in the Protein Data Bank (accession code 1SDW). We thank C. Ogata and the staff at beamline X4A for assistance during data collection at the National Synchrotron Light Source (NSLS) and K. Karlin and J. Berg for discussions and critical review of this manuscript. Supported by NSF grant no. MCB-9982945 (L.M.A. and S.T.P.) and NIH grant no. DK32949 (B.A.E. and R.E.M.). Beamline X4A at the NSLS, a U.S. Department of Energy facility, is supported by the Howard Hughes Medical Institute.
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