Opposite roles of protein kinase C isoforms in proliferation, differentiation, apoptosis, and tumorigenicity of human HaCaT keratinocytes

Cellular and Molecular Life Sciences

Volumn 61, Issue 9, 2004, Pages 1095-1105

  Papp, H ^a   Czifra, G ^a   Bodo E ^a   Lazar J ^a   Kovacs I ^b   Aleksza, M ^a   Juhasz I ^a   Acs P ^c   Sipka, S ^a   Kovacs L ^a   Blumberg, P M ^c   Biro T ^a  

^a UNIVERSITY OF DEBRECEN   (Hungary)

^b Kenezy Hospital Ltd   (Hungary)

^c NATIONAL CANCER INSTITUTE   (United States)

Author keywords

Differentiation; HaCaT; Human keratinocyte; Isoenzyme; Proliferation; Protein kinase C; Recombinant overexpression; Tumorigenesis

Indexed keywords

ISOENZYME; ISOPROTEIN; PROTEIN KINASE C;

APOPTOSIS; ARTICLE; CELL DIFFERENTIATION; CELL LINE; CELL PROLIFERATION; CONTROLLED STUDY; ENZYME ACTIVITY; HUMAN; HUMAN CELL; IMMUNE DEFICIENCY; IN VITRO STUDY; KERATINOCYTE; MOLECULAR BIOLOGY; PROTEIN FUNCTION; PROTEIN INDUCTION; TUMOR GROWTH;

ANIMALS; APOPTOSIS; CELL DIFFERENTIATION; CELL DIVISION; HUMANS; ISOENZYMES; KERATINOCYTES; MICE; MICE, SCID; PROTEIN KINASE C; SKIN NEOPLASMS;

EID: 2442482586     PISSN: 1420682X     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00018-004-4014-2     Document Type: Article

References (40)

1. Nishizuka Y. (1988) The molecular heterogeneity of protein kinase C and its implication for cellular regulation. Nature 334: 661-665
2. Ohno S., Akita Y., Hata A., Osada S., Kubo K., Kohno Y. et al. (1991) Structural and functional diversities of a family of signal transducing protein kinases, protein kinase C family; two distinct classes of PKC, conventional cPKC and novel nPKC. Adv. Enzyme Regul. 31: 287-303
3. Nishizuka Y. (1992) Intracellular signaling by hydrolysis of phospholipids and activation of protein kinase C. Science 258: 607-614
4. Gutcher I., Webb P. R. and Anderson N. G. (2003) The isoform-specific regulation of apoptosis by protein kinase C. Cell. Mol. Life Sci. 60: 1061-1070
5. Goodnight J. A., Mischak H. and Mushinski J. F. (1994) Selective involvement of protein kinase C isozymes in differentiation and neoplastic transformation. Adv. Cancer Res. 64: 159-209
6. Goodnight J. A., Mischak H., Kolch W. and Mushinski J. F. (1995) Immunocytochemical localization of eight protein kinase C isozymes overexpressed in NIN 3T3 fibroblasts: isoform-specific association with microfilaments, Golgi, endoplasmic reticulum, and nuclear and cell membranes. J. Biol. Chem. 270: 9991-10001
7. Mischak H., Goodnight J. A., Kolch W., Martiny-Baron G. M., Schaechtle C., Kazanietz M. G. et al. (1993) Overexpression of protein kinase C-δ and -ε in NIH 3T3 cells induces opposite effects on growth, morphology, anchorage dependence, and tumorigenicity. J. Biol. Chem. 268: 6090-6096
8. Murray N. R., Baumgardner G. P., Burns D. J. and Fields A. P. (1993) Protein kinase C isotypes in human erythroleukemia (K562) cell proliferation and differentiation: evidence that beta II protein kinase C is required for proliferation. J. Biol. Chem. 268: 15847-15853
9. Brodie C., Kuperstein I., Ács P. and Blumberg P. M. (1998) Differential role of specific PKC isoforms in the proliferation of glial cells and the expression of the astrocytic markers GFAP and glutamine synthetase. Mol. Brain Res. 56: 108-117
10. Lee Y. S., Yuspa S. H. and Dlugosz A. A. (1998) Differentiation of cultured human epidermal keratinocytes at high cell densities is mediated by endogenous activation of the protein kinase C pathway. J. Invest. Dermatol. 111: 762-766
11. Bikle D. D., Ng D., Tu C. L., Oda Y. and Xie Z. (2001) Calcium- and vitamin D-regulated keratinocyte differentiation. Mol. Cell. Endocrinol. 177: 161-171
12. Jansen A. P., Dreckschmidt N. E., Verwiebe E. D., Wheeler D. L., Oberley T. D. and Verma A. K. (2001) Regulation of the induction of epidermal ornithine decarboxylase and hyperplasia to the different skin tumor-promotion susceptibilities of protein kinase C alpha, -delta, and -epsilon transgenic mice. Int. J. Cancer. 93: 635-643
13. Neill G. W., Ghali L. R., Green J. L., Ikram M. S., Philpott M. P. and Quinn A. G. (2003) Loss of protein kinase C alpha expression may enhance the tumorigenic potential of Gli1 basal cell carcinoma. Cancer Res. 63: 4692-4697
14. Li L., Lorenzo P. S., Bogi K., Blumberg P. M. and Yuspa S. H. (1999) Protein kinase Cδ targets mitochondria, alters mitochondrial membrane potential, and induces apoptosis in normal and neoplastic keratinocytes when overexpressed by an adenoviral vector. Mol. Cell. Biol. 19: 8547-8558
15. Lee Y. S., Dlugosz A. A., McKay R., Dean N. M. and Yuspa S. H. (1997) Definition by specific antisense oligonucleotides of a role for protein kinase Cα in expression of differentiation markers in normal and neoplastic mouse epidermal keratinocytes. Mol. Carcinog. 18: 44-53
16. Boukamp P., Petrussevska R. T., Breitkreutz D., Hornung J., Markham A. and Fusenig N. E. (1988) Normal keratinization in a spontaneously immortalized aneuploid human keratinocyte cell line. J. Cell Biol. 106: 761-771
17. Breitkreutz D., Boukamp P., Ryle C. M., Stark H.-J., Roop D. R. and Fusenig N. E. (1991) Epidermal morphogenesis and keratin expression in c-Ha-ras-tranfected tumorgenic clones of the human HaCaT cell line. Cancer Res. 51: 4402-4409
18. Fusenig N. E. and Boukamp P. (1998) Multiple stages and genetic alterations in immortalization, malignant transformation, and tumor progression of human skin keratinocytes. Mol. Carcinog. 23: 144-158
19. Papp H., Czifra G., Lázár J., Boczán J., Gönczi M., Csernoch L. et al. (2003) Protein kinase C isozymes regulate proliferation and high cell density-mediated differentiation of HaCaT keratinocytes. Exp. Dermatol. 12: 811-824
20. Ács P., Bögi K., Marquez A. M., Lorenzo P. S., Bíró T., Szállási Z. et al. (1997) The catalytic domain of protein kinase C chimeras modulates the affinity and targeting of phorbol ester induced translocation. J. Biol. Chem. 272: 22148-22153
21. Ács P., Wang Q. J., Bögi K., Marquez A. M., Lorenzo P. S., Bíró T. et al. (1997) Both the catalytic and regulatory domains of protein kinase C chimeras modulate the proliferation properties of NIH 3T3 cells. J. Biol. Chem. 272: 28793-28799
22. Oláh Z., Lehel C., Jakab G. and Anderson W. B. (1994) A cloning and epsilon-epitope-tagging insert for the expression of polymerase chain reaction-generated cDNA fragments in Escherichia coli and mammalian cells. Anal. Biochem. 221: 94-102
23. Lázár J., Szabó T., Kovács L., Blumberg P. M. and Bíró T. (2003) Distinct features of recombinant vanilloid receptor-1 expressed in various expression systems. Cell. Mol. Life Sci. 60: 2228-2240
24. Laemmli U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685
25. Rose D. S. C., Maddox P. H. and Brown D. C. (1994) Which proliferation markers for routine immunohistology? A comparison of five antibodies. J. Clin. Pathol. 47: 1010-1014
26. Toullec D., Pianetti P., Coste H., Bellevergue P., Grand-Perret T., Ajakane M. et al. (1991) The bisindolylmaleimide GF 109203X is a potent and selective inhibitor of protein kinase C. J. Biol. Chem. 266: 15771-15781
27. Martiny-Baron G., Kazanietz M. G., Mischak H., Blumberg P. M., Kochs G., Hug H. et al. (1993) Selective inhibition of protein kinase C isozymes by the indolocarbazole Gö 6976. J. Biol. Chem. 268: 9194-9197
28. Gschwendt M., Müller H.-J., Kialbassa K., Zang R., Kittstein W, Rincke G. et al. (1994) Rottlerin, a novel protein kinase inhibitor. Biochem. Biophys. Res. Commun. 199: 93-98
29. Alessi D. R. (1997) The protein kinase C inhibitors Ro 318220 and GF 109203X are equally potent inhibitors of MAPKAP kinase-1β and p70 S6 kinase. FEBS Lett. 402: 121-123
30. Zang L. C., Ng D. C. and Bikle D. D. (2003) Role of protein kinase a in calcium induced keratinocyte differentiation: defective regulation in squamous cell carcinoma. J. Cell. Physiol. 195: 249-259
31. 3. Arch. Dermatol. Res. 292: 455-462
32. Fisher G. J., Tavakkol A., Leach K., Burns D., Basta P., Loomis C. et al. (1993) Differential expression of protein kinase C isoenzymes in normal and psoriatic adult human skin: reduced expression of protein kinase C-betaII in psoriasis. J. Invest. Dermatol. 101: 553-559
33. Denning M. F., Wang Y., Nickoloff B. J. and Wrone-Smith T. (1998) Protein kinase Cdelta is activated by caspase-dependent proteolysis during ultraviolet radiation-induced apoptosis of human keratinocytes. J. Biol. Chem. 273: 29995-30002
34. Denning M. F., Wang Y., Tibudan S., Alkan S., Nickoloff B. J. and Qin J. Z. (2002) Caspase activation and disruption of mitochondrial membrane potential during UV radiation-induced apoptosis of human keratinocytes requires activation of protein kinase C. Cell Death Differ. 9: 40-52
35. Fukunaga M., Oka M., Ichihashi M., Yamamoto T., Matsuzaki H. and Kikkawa U. (2001) UV-induced tyrosine phosphorylation of PKC delta and promotion of apoptosis in the HaCaT cell line. Biochem. Biophys. Res. Commun. 289: 573-579
36. Geiges D., Marks F. and Gschwendt M. (1995) Loss of protein kinase Cδ from human HaCaT keratinocytes upon ras transfection is mediated by TGFα. Exp. Cell. Res. 219: 299-303
37. Dietrich C., Gumpert N., Heit I., Borchert-Stuchltrager M., Oesch F. and Wieser R. (2001) Rottlerin induces a transformed phenotype in human keratinocytes. Biochem. Biophys. Res. Commun. 282: 575-579
38. Xie X., Brunner N., Jensen G., Albrectsen J., Gotthardsen B. and Rygaard J. (1992) Comparative studies between nude and scid mice on the growth and metastatic behavior of xenografted human tumors. Clin. Exp. Metastasis 10: 201-210
39. Taghian A., Budach W., Zietman A., Freeman J., Gioioso D. and Suit H. D. (1993) Quantitative comparison between the transplantability of human and murine tumors into the brain of NCr/Sed-nu/nu nude and severe combined immunodeficient mice. Cancer Res. 53: 5018-5021
40. Kubota T., Yamaguchi H., Watanabe M., Yamamoto T., Takahara T., Takeuchi T. et al. (1993) Growth of human tumor xenografts in nude mice and mice with severe combined immunodeficiency (SCID). Surg. Today 23: 375-377