Comparison of Bioenergetic Activity of Primary Porcine Hepatocytes Cultured in Four Different Media

Cell Transplantation

Volumn 13, Issue 3, 2004, Pages 213-229

  Dabos, Konstantinos J ^a,b   Nelson, Leonard J ^a   Hewage, Chandralal H ^a   Parkinson, John A ^a   Howie, A Forbes ^a   Sadler, Ian H ^a   Hayes, Peter C ^a   Plevris, John N ^a  

^a UNIVERSITY OF EDINBURGH   (United Kingdom)

^b ROYAL INFIRMARY OF EDINBURGH   (United Kingdom)

Author keywords

Albumin synthesis; Glucolysis; Gluconeogenesis; Ketogenesis; Ureagenesis

Indexed keywords

ALBUMIN; ANTIBIOTIC AGENT; CYTOCHROME P450; HORMONE; SUCCINIC ACID; UREA;

ANIMAL CELL; ARTICLE; BIOENERGY; CELL ISOLATION; CELL VIABILITY; COMPARATIVE STUDY; CONCENTRATION RESPONSE; CONTROLLED STUDY; CULTURE MEDIUM; ENERGY METABOLISM; GLUCONEOGENESIS; GLYCOLYSIS; KETOGENESIS; LIVER CELL; METABOLIC RATE; NONHUMAN; PRIORITY JOURNAL; PROTEIN SYNTHESIS; PROTON NUCLEAR MAGNETIC RESONANCE; SWINE; UREA CYCLE;

ANIMALIA; SUIDAE; SUS SCROFA;

EID: 2442477557     PISSN: 09636897     EISSN: None     Source Type: Journal    
DOI: 10.3727/000000004783984007     Document Type: Article

References (48)

1. Bader, A.; De Bartolo, D.; Haverich, A. High level benzodiazepine and ammonia clearance by flat membrane bioreactors with porcine liver cells. J. Biotechnol. 81:95-105; 2000.
2. Brodskii, V. Y.; Nechaeva, N. V.; Terskikh, V. V.; Novikova, T. E.; Gvazava, I. G.; Fateeva, V. I. A serum-free medium that preserves normal morphology and a high level of protein synthesis in hepatocytes. Izv. Acad. Nauk Ser. Biol. 4:398-401; 1996.
3. Chesne, C.; Guyomard, C.; Fautrel, A.; Poullain, M-G.; Fremond, B.; De Jong, C.; Guillouzo, A. Viability and function of primary culture of adult hepatocytes from various animal species and human beings after cryopreservation. Hepatology 18:685-690; 1993.
4. Coldham, N. G.; Biancotto, G.; Montesissa, C.; Howells, L. C.; Sauer, M. J. Utility of isolated hepatocytes and radio-HPLC-MSn for the analysis of the metabolic fate of 19-nortestosterone laurate in cattle. Analyst 123:2589-2594; 1998.
5. Dabos, K. J.; Nelson, L. J.; Bradnock, T. J.; Parkinson, J. A.; Sadler, I. H.; Hayes, P. C.; Plevris, J. N. The simulated microgravity environment maintains key metabolic functions and promotes aggregation of primary porcine hepatocytes. Biochim. Biophys. Acta 1526:119-130; 2001.
6. 1H NMR spectroscopy as a tool to evaluate key metabolic functions of primary porcine hepatocytes after cryopreservation. NMR Biomed. 15:241-250; 2002.
7. De Bartolo, D.; Jarosch-von Schweder, G.; Haverich, A.; Bader, A. A novel full-scale flat bioreactor utilising porcine hepatocytes: Cell viability and tissue specific functions. Biotechnol. Prog. 16:102-108; 2000.
8. Denton, R. M.; Halestrap, A. P. Regulation of pyruvate metabolism in mammalian tissues. Essays Biochem. 15:37-44; 1978.
9. Desille, M.; Corcos, L.; L'Helgouac'h, A.; Fremond, B.; Campion, J. P.; Guillouzo, A.; Clement, B. Detoxifying activity in pig livers and hepatocytes intended for xenotherapy. Transplantation 68:1437-1443; 1999.
10. Donato, M. T.; Castell, J. V.; Gomez-Lechon, M. J. Characterization of drug metabolising activities in pig hepatocytes for use in bioartificial liver devices: Comparison with other hepatic cellular models. J. Hepatol. 31:542-549; 1999.
11. Dunn, J. C.; Tompkins, R. G.; Yarmush, M. L. Hepatocytes in collagen sandwich: Evidence for transcriptional and translational regulation. J. Cell Biol. 116:1043-1053; 1992.
12. Flendrig, L. M.; La Soe, J. M.; Jorning, J. J. A.; Steenbeek, A.; Karlsen, O. T.; Bovee, W. M. M. J.; Ladiges, N. C. J. J.; te Velde, A. A.; Chamuleau, R. A. F. M. In vitro evaluation of a novel bioreactor based on an integral oxygenator and a spirally wound nonwoven polyester matrix for hepatocyte culture as small aggregates. J. Hepatol. 26:1379-1392; 1997.
13. Flendrig, L. M.; Sommeijer, D.; Ladiges, N. C. J. J.; te Velde, A. A.; Maas, M. A. W.; Jorning, G. G. A.; Daalhuisen, J.; Chamuleau, R. A. Commercially viable media for flushing extracorporeal bioartificial liver systems prior to connection with the patient's circulation: An in vitro comparative study in two and three dimensional porcine hepatocyte cultures. Int. J. Artif. Organs 21:467-472; 1998.
14. Foxall, P. J. D.; Price, R. G.; Jones, J. K.; Neild, G. H.; Thompson, F. D.; Nicholson, J. K. High resolution proton magnetic resonance spectroscopy of cyst fluids from patients with polycystic kidney disease. Biochim. Biophys. Acta 1138:305-311; 1992.
15. Fuchs, M.; Gerlach, J.; Encke, J.; Unger, J.; Smith, M.; Neuhaus, P.; Riedel, E. Amino acid metabolism in a hybrid liver support bioreactor. Int. J. Artif. Organs 17:663-669; 1994.
16. Gerlach, J. C.; Brombacher, J.; Kloppel, K.; Schnoy, N.; Neuhaus, P. Comparison of four methods of mass hepatocyte isolation from pig and human livers. Transplantation 57:1318-1322; 1994.
17. Gerlach, J.; Kloppel, K.; Stoll, P.; Vienken, J.; Muller, C. Gas supply across membranes in bioreactors for hepatocyte cultures. Artif. Organs 14:328-333; 1990.
18. Gerlach, J.; Kloppel, K.; Schauwecker, H. H.; Tauber, R.; Muller, C.; Bucherl, E. Use of hepatocytes in adhesion and suspension cultures for liver support bioreactors. Int. J. Artif. Organs 12:788-792; 1989.
19. Gerlach, J. C.; Fuchs, M.; Smith, M. D.; Bornemann, R.; Encke, J.; Neuhaus, P.; Riedel, E. Is a clinical application of hybrid liver support systems limited by an initial disorder in cellular amino-acid and α-ketoacid metabolism rather than by later gradual loss of primary hepatocyte function. Transplantation 62:224 228; 1996.
20. Grunnet, N.; Vind, C.; Dich, J. Synergistic effect of insulin and glucocorticoid on albumin secretion in cultured hepatocytes. Acta Physiol. Scand. 133:593 594; 1988.
21. Guzik, K.; Reiss, K.; Korohoda, W. Survival and phenol red metabolism in chick-embryo hepatocytes cultured in the serum-free medium 199 supplemented with dextran T-500 and antioxidants. Folia Histochem. Cytobiol. 27:125-127; 1989.
22. Joly, A.; Desjardins, J. F.; Fremond, B.; Desille, M.; Campion, J. P.; Malledant, Y.; Lebreton, Y.; Semana, G.; Edwards-Levy, F.; Levy, M.-C.; Clement, B. Survival proliferation and functions of porcine hepatocytes encapsulated in coated algenate beads: A step towards a reliable bioartificial liver. Transplantation 63:795-803; 1997.
23. Kano, J.; Tokiwa, T.; Zhou, X.; Kodama, M. Colonial growth and differentiation of epithelial cells derived from abattoir adult porcine livers. J. Gastroenterol. Hepatol. 13: S62-S69; 1998.
24. Koebe, H. G.; Schildberg, F. W. Isolation of porcine hepatocytes from slaughterhouse organs. Int. J. Artif. Organs 19:53-60; 1996.
25. Kong, L. B.; Chen, S.; Demetriou, A. A.; Rozga, J. Matrix-induced liver cell aggregates (MILCA) for bioartificial liver use. Int. J. Arif. Organs 19:72-78; 1996.
26. Lazar, A.; Mann, H. J.; Remmel, R. P.; Shatford, R. A.; Cerra, F. B.; Hu, W. S. Extended liver specific functions of porcine hepatocyte spheroid entrapped in collagen gel. In Vitro Cell Dev. Biol. Anim. 31:340-346; 1995.
27. Lindon, J. C.; Nicholson, J. K.; Everet, J. R. NMR spectroscopy of biofluids. Annu. Rep. NMR Spectroscopy 38:1-87; 1999.
28. Meijer, A. J.; Lamers, W. H.; Chamuleau, R. A. Nitrogen metabolism and ornithine cycle function. Physiol. Rev. 70:701-748; 1990.
29. Michalopoulos, G. K. Liver regeneration: Molecular mechanisms in growth control. FASEB J. 4:176-187; 1990.
30. Mitaka, T. The current status of primary hepatocyte culture. Int. J. Exp. Pathol. 79:393-409; 1998.
31. Monshouwer, M.; van't Klooster, G. A. E.; Nijmeijer, S. M.; Witkamp, R. F.; Van Miert, A. Characterization of cytochrome P450 isoenzymes in primary cultures of pig hepatocytes. Toxicol. In Vitro 12:715-723; 1998.
32. Naik, S.; Trenkler, T.; Santangini, H.; Mullon, C. J. P.; Solomon, B. A. ; Pan, J.; Jauregui, O. Functional recovery of porcine hepatocytes after hypothermic or cryogenic preservation for liver support systems. Cell Transplant. 6:447-454; 1997.
33. Nelson, L. J.; Newsome, P. N.; Howie, A. F.; Hadoke, P. W. F.; Dabos, K. J.; Walker, S. W.; Hayes, P. C.; Plevris, J. N. An improved ex-vivo method of primary porcine hepatocyte isolation for use in bioartificial liver systems. Eur. J. Gastroenterol. Hepatol. 12:923-930; 2000.
34. Newsome, P. N.; Plevris, J. P.; Nelson, L. J.; Hayes, P. C. Animal models of fulminant hepatic failure. Liver Transpl. Surg. 6:21-31; 2000.
35. Olsen, A. K.; Hansen, K. T.; Friis, C. Pig hepatocytes as an in vitro model to study the regulation of human CYP3A4: Prediction of drug-drug interaction with 17 alpha-ethinyloestradiol. Chem. Biol Interact. 107:93-108; 1997.
36. Plevris, J. N.; Schina, M.; Hayes, P. C. Review article: The management of acute liver failure. Aliment Pharmacol. Ther. 12:405-418; 1998.
37. Sajiki, T.; Iwata, H.; Paek, H. J.; Tosha, T.; Fujita, S.; Ueda, Y.; Park, Y. G.; Zhu, B.; Satoh, S.; Ikai, I.; Yamaoka, Y.; Ikada, Y. Morphologic studies of hepatocytes entrapped in hollow fibers of a bioartficial liver. ASAIO J. 46:49-55; 2000.
38. Scott, C. D.; Baxter, R. C. Synthesis of the acid-labile subunit of the growth-hormone-dependent insulin-like-growth-factor-binding protein complex by rat hepatocytes in culture. Biochem. J. 275:441-446; 1991.
39. Seglen, P. O. Autoregulation of glycolysis, respiration, gluconeogenesis and glycogen synthesis in isolated parenchymal rat liver cells under aerobic and anaerobic conditions. Biochim. Biophys. Acta 338:317-338; 1974.
40. Seifter, S.; Englard, S. Energy metabolism. In: Arias, I. M.; Boyer, J. L.; Faust, N.; Jakoby, W. B.; Scahcter, D. A.; Shafritz, D. A., eds. The liver: Biology and pathobiology, 3rd ed. New York: Raven Press; 1994:323-364.
41. Shiraha, H.; Koide, N.; Hada, H.; Ujike, K.; Nakamura, M.; Shinji, T.; Gotoh, S.; Tsuji, T. Improvement of serum amino acid profile in hepatic failure with the bioartificial liver using multicellular hepatocyte spheroids. Biotechnol. Bioeng. 50:416-421; 1996.
42. Sun, R.; Gimbel, H. V.; Liu, S.; Guo, D. L.; Hollenberg, M. D. Effect of diclofenac sodium and dexamethasone on cultured human Tenon's capsule fibroblasts. Ophthalmic Surg. Lasers 30:382-388; 1999.
43. te Velde, A. A.; Ladiges, N. C. J. J.; Flendrig, L. M.; Chamuleau, R. A. F. M. Functional activity of isolated pig hepatocytes attached to different extracellular matrix substrates: Implications for application of pig hepatocytes in a bioartificial liver. J. Hepatol. 23:184-192; 1995.
44. Tsiaoussis, J.; Newsome, P. N.; Nelson, L. J.; Hayes, P. C.; Plevris, J. N. Which hepatocyte will it be? Hepatocyte choice for bioartificial liver support systems. Liver Transpl. 7:2-10; 2001.
45. Wang, L. S.; Sun, J. H.; Li, L.; Mears, D.; Horvat, M.; Sheil, A. G. R. Comparison of porcine hepatocytes with human hepatoma (C3A) cells for use in a bioartificial liver support system. Cell Transplant. 7:459-468; 1998.
46. Watanabe, F. D.; Mullon, C. J. P.; Hewitt, W. R.; Arkadopoulos, N.; Kahaku, E.; Eguchi, S.; Khalili, T.; Amaout, W.; Shackleton, C. R.; Rozga, J.; Solomon, B.; Demetriou, A. A. Clinical experience with a bioartificial liver in the treatment of severe liver failure-a phase I clinical trial. Ann. Surg. 225:484-491; 1997.
47. Watts, P.; Smith, M. D.; Edwards, I.; Zammit, V.; Brown, V.; Grant, M. H. The influence of medium composition on the maintenance of cytochrome P-450, glutathione content and urea synthesis: A comparison of rat and sheep hepatocyte cultures. Hepatology 23:605-612; 1995.
48. Wegner, H.; Shareck, W.; Bayer-Helms, H.; Gebhardt, R. Different proliferative potential of rat and pig hepatocytes in pure primary culture and coculture. Eur. J. Cell Biol. 58:411-417; 1992.