Survival, proliferation, and functions of porcine hepatocytes encapsulated in coated alginate beads: A step toward a reliable bioartificial liver

Transplantation

Volumn 63, Issue 6, 1997, Pages 795-803

  Joly, Agnès ^a   Desjardins, Jean François ^a   Fremond, Benjamin ^a   Desille, Mireille ^a   Campion, Jean Pierre ^a   Malledant, Yannick ^a   Lebreton, Yves ^b   Semana, Gilbert ^c   Edwards Levy, Florence ^d   Levy, Marie Christine ^d   Clement, Bruno ^a,e  

^a UNIVERSITÉ DE RENNES 1   (France)

^b CEMAGREF   (France)

^c Etablissement de Transfus Sanguine   (France)

^d UNIVERSITÉ DE REIMS CHAMPAGNE ARDENNE   (France)

^e INSERM   (France)

Author keywords

[No Author keywords available]

Indexed keywords

ALGINIC ACID PROPYLENE GLYCOL ESTER; ETHOXYRESORUFIN DEETHYLASE; HUMAN SERUM ALBUMIN; IMMUNOGLOBULIN G; NIFEDIPINE; PARACETAMOL; PHENACETIN; PROCAINAMIDE; PYRIDINE; THYMIDINE DERIVATIVE;

ACYLATION; ANIMAL CELL; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; ARTIFICIAL MEMBRANE; CELL ISOLATION; CELL PROLIFERATION; CELL SURVIVAL; DNA SYNTHESIS; FEMALE; HLA SYSTEM; LIVER CELL; LIVER REGENERATION; LIVER TRANSPLANTATION; MICROENCAPSULATION; NONHUMAN; PRIORITY JOURNAL; SWINE;

ACETAMINOPHEN; ALGINATES; ANIMALS; BIOTRANSFORMATION; CAPSULES; CELL DIVISION; CELL SURVIVAL; CELLS, CULTURED; CULTURE TECHNIQUES; CYTOCHROME P-450 ENZYME SYSTEM; DNA; FEMALE; GLUCURONIC ACID; HEXURONIC ACIDS; HUMANS; LIVER; LIVER DISEASES; LIVER, ARTIFICIAL; MICROSCOPY, ELECTRON; MICROSCOPY, ELECTRON, SCANNING; PROCAINAMIDE; PROTEIN BIOSYNTHESIS; SERUM ALBUMIN; SWINE;

EID: 0030891942     PISSN: 00411337     EISSN: None     Source Type: Journal    
DOI: 10.1097/00007890-199703270-00002     Document Type: Article

References (32)

1. Hoofnagle JH, Carithers RL, Shapiro C, Ascher N. Fulminant hepatic failure: summary of a workshop. Hepatology 1995; 21: 240.
2. Lidofsky SD. Liver transplantation for fulminant hepatic failure. Gastroenterol Clin North Am 1993; 22: 257.
3. O'Grady JG, Gimson AES, O'Brien CJ, Pucknell A, Hughes RD, Williams R. Controlled trials of charcoal hemoperfusion and prognostic factors in fulminant hepatic failure. Gastroenterology 1988; 94: 1192.
4. Dixit V. Transplantation of isolated hepatocytes and their role in extrahepatic life support systems. Scan J Gastroenterol 1995; 30 (suppl): 101.
5. Sussman NL, Gislason GT, Conlin CA, Kelly JH. The Hepatix extracorporeal liver assist device: initial clinical experience. Artif Organs 1994; 18: 390.
6. Gerlach JC. Use of hepatocyte cultures for liver support bioreactors. Adv Exp Med Biol 1994; 368: 165.
7. Nyberg SL, Shatford RA, Peshwa MV, White JG, Cerra FB, Hu WS. Evaluation of a hepatocyte-entrapment hollow fiber bioreactor: a potential bioartificial liver. Biotechnol Bioeng 1993; 41: 194.
8. Lazar A, Peshwa MV, Wu FJ, Chi CM, Cerra FB, Hu WS. Formation of porcine hepatocytes spheroids for use in a bioartificial liver. Cell Transplant 1995; 4: 259.
9. Rozga J, Williams F, Ro MS, et al. Development of a bioartificial liver: properties and function of a hollow-fiber module inoculated with liver cells. Hepatology 1993; 17: 258.
10. Demetriou AA, Rozga J, Podesta L, et al. Early clinical experience with a hybrid bioartificial liver. Scand J Gastroenterol 1995; 30 (suppl): 111.
11. Frémond B, Malandain C, Guyomard C, Chesné C, Guillouzo A, Campion JP. Correction of bilirubin conjugation in the Gunn rat using hepatocytes immobilized in alginate gel beads as an extracorporeal bioartificial liver. Cell Transplant 1993; 2: 453.
12. Miura Y, Akimoto T, Yagi K. Liver functions in hepatocytes entrapped within calcium alginate. Ann NY Acad Sci 1988; 542: 521.
13. Tompkins RG, Carter EA, Carlson JD, Yarmush ML. Enzymatic functions of alginate immobilized rat hepatocytes. Biotechnol Bioeng 1988; 31: 11.
14. Guyomard C, Rialland L, Frémond B, Chesné C, Guillouzo A. Influence of alginate gel entrapment and cryopreservation on survival and xenobiotic metabolism capacity of rat hepatocytes. Toxicol Appl Pharmacol 1996; 141: 349.
15. Guguen-Guillouzo C, Guillouzo A. Methods for preparation of adult and fetal hepatocytes. In: Guillouzo A, Guguen-Guillouzo C, eds. Isolated and cultured hepatocytes. Paris: Les Editions INSERM, John Libbey Eurotext, 1986: 1.
16. Levy MC, Edwards-Levy F. Coating alginate beads with cross-linked biopolymers: a novel method based on a transacylation reaction. J Microencapsulation 1996; 13: 169.
17. Guillouzo A, Chesné C. Xenobiotic metabolism in epithelial cell cultures. In: Shaw AJ, ed. Epithelial cell culture: a practical approach. Oxford: Oxford University Press, 1996: 67.
18. Burke MD, Mayer RT. Differential effects of phenobarbitone and 3-methylcholanthrene induction on the hepatic microsomal metabolism and cytochrome P-450-binding of phenoxazone and a homologous series of its N-alkyl ethers (alkoxyresorufin). Chem Biol Interact 1983; 45: 243.
19. Guengerich FP, Martin MV, Beaune PH, Kremers P, Wolff T, Waxman DJ. Characterization of rat and human liver microsomal cytochrome P-450 forms involved in nifedipine oxidation, a prototype for genetic polymorphism in oxidative drug metabolism. J Biol Chem 1986; 261: 5051.
20. Dislerath LM, Reilly PE, Martin MV, Davis GG, Wilkinson GR, Guengerich FP. Purification and characterization of the human liver cytochrome P-450 involved in debrisoquine 4-hydroxylation and phenacetin O-deethylation, two prototypes for genetic polymorphism in oxidative drug metabolism. J Biol Chem 1985; 260: 9057.
21. Moldeus P. Paracetamol metabolism and toxicity in isolated hepatocytes from rat and mouse. Biochem Pharmacol 1978; 27: 2859.
22. Rocco RM, Abbott DC, Giese RW, Karger BL. Analysis for procainamide and N-acetyl procainamide in plasma or serum by high-performance chromatography. Clin Chem 1977; 23: 705.
23. Sielaff TD, Hu MY, Rao S, et al. A technique for porcine hepatocyte harvest and description of differentiated metabolic functions in static culture. Transplantation 1995; 59: 1459.
24. Gerlach JC, Brombacher J, Kloppel K, Schnoy N, Neuhaus P. Comparison of four methods for mass hepatocyte isolation from pig and human livers. Transplantation 1994; 57: 1318.
25. Morsiani E, Rozga J, Scott HC, et al. Automated large-scale production of porcine hepatocytes for bioartificial liver support. Transplant Proc 1994; 26: 3505.
26. Nordlinger B, Bouma ME, Wang SR, et al. High-yield preparation of porcine hepatocytes for long survival after transplantation in the spleen. Eur Surg Res 1985; 17: 377.
27. Clément B, Guguen-Guillouzo C, Campion JP, Glaise D, Bourel M, Guillouzo A. Long-term cocultures of adult human hepatocytes with rat liver epithelial cells: modulation of albumin secretion and accumulation of extracellular matrix. Hepatology 1984; 4: 373.
28. Loyer P, Cariou S, Glaise D, Bilodeau M, Baffet G, Guguen-Guillouzo C. Entry and progression of normal rat hepatocytes in G1 phase are independent of growth factors until a restriction point located in mid G1. J Biol Chem 1996; 271: 11484.
29. Wegner H, Schareck W, Bayer-Helms H, Gebhardt R. Different proliferative potential of rat and pig hepatocytes in primary culture and coculture. Eur J Cell Biol 1992; 58: 411.
30. Lim F, Sun AM. Microencapsulated islets as bioartificial endocrine pancreas. Science 1980; 210: 908.
31. Tanaka H, Matsumara M, Veliky IA. Diffusion characteristics of substrates in Ca-alginate gel beads. Biotechnol Bioeng 1984; 26: 53.
32. Arnaout WS, Moscioni AD, Barbour RL, Demetriou AA. Development of bioartificial liver: bilirubin conjugation in Gunn rats. J Surg Res 1990; 48: 379.