Development of a novel cytochrome P450 bioaffinity detection system coupled online to gradient reversed-phase high-performance liquid chromatography

Journal of Biomolecular Screening

Volumn 10, Issue 5, 2005, Pages 427-436

  Kool, Jeroen ^a   Van Liempd, Sebastiaan M ^a   Ramautar, Rawi ^a   Schenk, Tim ^b   Meerman, John H N ^a   Irth, Hubertus ^b   Commandeur, Jan N M ^a   Vermeulen, Nico P E ^a  

^a VU UNIVERSITY AMSTERDAM   (Netherlands)

^b Kiadis BV   (Netherlands)

Author keywords

Cytochrome P450; Enzyme affinity detection (EAD); Fluorescence; High resolution screening (HRS); Online bioaffinity assay

Indexed keywords

9 HYDROXYELLIPTICINE; ACETONITRILE; ALPHA NAPHTHOFLAVONE; BETA NAPHTHOFLAVONE; CAFFEINE; CYTOCHROME P450; CYTOCHROME P450 1A1; CYTOCHROME P450 1A2; ELLIPTICINE; ETHOXYRESORUFIN; FLUVOXAMINE; METHANOL; NATRIURETIC FACTOR; PHENACETIN;

ANIMAL TISSUE; ARTICLE; BINDING AFFINITY; ENZYME BINDING; FLOW INJECTION ANALYSIS; IC 50; LIVER MICROSOME; NONHUMAN; PRIORITY JOURNAL; RAT; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; SCREENING; SIGNAL NOISE RATIO;

ANIMALS; AUTOMATION; BENZOFLAVONES; BIOLOGICAL ASSAY; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CHROMATOGRAPHY, LIQUID; CYTOCHROME P-450 CYP1A1; CYTOCHROME P-450 CYP1A2; CYTOCHROME P-450 ENZYME SYSTEM; DOSE-RESPONSE RELATIONSHIP, DRUG; DRUG EVALUATION, PRECLINICAL; DRUG INTERACTIONS; INHIBITORY CONCENTRATION 50; LIGANDS; LIVER; MICROSCOPY, FLUORESCENCE; MICROSOMES, LIVER; RATS; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; TECHNOLOGY, PHARMACEUTICAL; TEMPERATURE; TIME FACTORS;

MAMMALIA;

EID: 24144479994     PISSN: 10870571     EISSN: None     Source Type: Journal    
DOI: 10.1177/1087057105274904     Document Type: Article

References (27)

1. Glue P, Clement RP: Cytochrome P450 enzymes and drug metabolism-basic concepts and methods of assessment. Cell Mol Neurobiol 1999;19:309-323.
2. Ekins S, Ring BJ, Grace J, McRobie-Belle DJ, Wrighton SA: Present and future in vitro approaches for drug metabolism. J Pharmacol Toxicol Methods 2000;44:313-324.
3. Guengerich FP: Common and uncommon cytochrome P450 reactions related to metabolism and chemical toxicity. Chem Res Toxicol 2001;14:611-650.
4. Black SD: Membrane topology of the mammalian P450 cytochromes. FASEB J 1992;6:680-685.
5. Krishna DR, Klotz U: Extrahepatic metabolism of drugs in humans. Clin Pharmacokinet 1994;26:144-160.
6. Goeptar AR, Scheerens H, Vermeulen NP: Oxygen and xenobiotic reductase activities of cytochrome P450. Crit Rev Toxicol 1995;25:25-65.
7. Lin JH, Lu AY: Role of pharmacokinetics and metabolism in drug discovery and development. Pharmacol Rev 1997;49:403-449.
8. Vermeulen NP: Prediction of drug metabolism: the case of cytochrome P450 2D6. Curr Top Med Chem 2003;3:1227-1239.
9. Rodrigues AD: Preclinical drug metabolism in the age of high-throughput screening: an industrial perspective. Pharm Res 1997;14:1504-1510.
10. Bu HZ, Knuth K, Magis L, Teitelbaum P: High-throughput cytochrome P450 (CYP) inhibition screening via a cassette probe-dosing strategy: V. Validation of a direct injection/on-line guard cartridge extraction-tandem mass spectrometry method for CYP1A2 inhibition assessment. Eur J Pharm Sci 2001;12:447-452.
11. Stresser DM, Turner SD, Blanchard AP, Miller VR Crespi CL: Cytochrome P450 fluorometric substrates: identification of isoform-selective probes for rat CYP2D2 and human CYP3A4. Drug Metab Dispos 2002;30:845-852.
12. Venhorst J, Onderwater RC, Meerman JH, Vermeulen NR Commandeur JN: Evaluation of a novel high-throughput assay for cytochrome P450 2D6 using 7-methoxy-4-(aminomethyl)-coumarin. Eur J Pharm Sci 2000;12:151-158.
13. Miller VP, Stresser DM, Blanchard AP, Turner S, Crespi CL: Fluorometric high-throughput screening for inhibitors of cytochrome P450. Ann N Y Acad Sci 2000;919:26-32.
14. Yamamoto T, Suzuki A, Kohno Y: High-throughput screening for the assessment of time-dependent inhibitions of new drug candidates on recombinant CYP2D6 and CYP3A4 using a single concentration method. Xenobiotica 2004;34:87-101.
15. Oosterkamp AJ, Villaverde Herraiz MT, Irth H, Tjaden UR, van der Greef J: Reversed-phase liquid chromatography coupled on-line to receptor affinity detection based on the human estrogen receptor. Anal Chem 1996;68:1201-1206.
16. Schobel U, Frenay M, van Elswijk DA, McAndrews JM, Long KR, Olson LM, et al: High resolution screening of plant natural product extracts for estrogen receptor alpha and beta binding activity using an online HPLC-MS biochemical detection system. J Biomol Screen 2001;6:291-303.
17. Schenk T, Breel GJ, Koevoets P, van den Berg S, Hogenboom AC, Irth H, et al: Screening of natural products extracts for the presence of phosphodiesterase inhibitors using liquid chromatography coupled online to parallel biochemical detection and chemical characterization. J Biomol Screen 2003;8:421-429.
18. Oosterkamp AJ, Irth H, Heintz L, Marko-Varga G, Tjaden UR, van der Greef J: Simultaneous determination of cross-reactive leukotrienes in biological matrices using on-line liquid chromatography immunochemical detection. Anal Chem 1996;68:4101-4106.
19. Schenk T, Irth H, Marko-Varga G, Edholm LE, Tjaden UR, van der Greef J: Potential of on-line micro-LC immunochemical detection in the bioanalysis of cytokines. J Pharm Biomed Anal 2001;26:975-985.
20. van Breemen RB, Nikolic D, Bolton JL: Metabolic screening using on-line ultrafiltration mass spectrometry. Drug Metab Dispos 1998;26:85-90.
21. Johnson BM, Nikolic D, van Breemen RB: Applications of pulsed ultrafiltration-mass spectrometry. Mass Spectrom Rev 2002;21:76-86.
22. Ingkaninan K, de Best CM, van der Heijden R, Hofte AJ, Karabatak B, Irth H, et al: High-performance liquid chromatography with on-line coupled UV, mass spectrometric and biochemical detection for identification of acetylcholinesterase inhibitors from natural products. J Chromatogr A 2000;872:61-73.
23. Ingkaninan K, Hazekamp A, de Best CM, Irth H, Tjaden UR, van der Heijden R, et al: The application of HPLC with on-line coupled UV/MS-biochemical detection for isolation of an acetylcholinesterase inhibitor from narcissus "Sir Winston Churchill." J Nat Prod 2000;63:803-806.
24. Schenk T, Appels NM, van Elswijk DA, Irth H, Tjaden UR, van der Greef J: A generic assay for phosphate-consuming or -releasing enzymes coupled online to liquid chromatography for lead finding in natural products. Anal Biochem 2003;316:118-126.
25. Rooseboom M, Commandeur JN, Floor GC, Rettie AE, Vermeulen NP: Selenoxidation by flavin-containing monooxygenases as a novel pathway for beta-elimination of selenocysteine Se-conjugates. Chem Res Toxicol 2001;14:127-134.
26. Burke MD, Mayer RT: Ethoxyresorufin: direct fluorimetric assay of a microsomal O-dealkylation which is preferentially inducible by 3-methylcholanthrene. Drug Metab Dispos 1974;2:583-588.
27. Oosterkamp AJ, Irth H, Beth M, Unger KK, Tjaden UR, van de Greef J: Bioanalysis of digoxin and its metabolites using direct serum injection combined with liquid chromatography and on-line immunochemical detection. J Chromatogr B Biomed Appl 1994;653:55-61.