A generic assay for phosphate-consuming or -releasing enzymes coupled on-line to liquid chromatography for lead finding in natural products

Analytical Biochemistry

Volumn 316, Issue 1, 2003, Pages 118-126

  Schenk, T ^a   Appels, N M G M ^b   Van Elswijk, D A ^a   Irth, H ^a,c   Tjaden, U R ^d   Van Der Greef, J ^d  

^a Netherlands Cancer Institute ^*  (Netherlands)

^b NETHERLANDS CANCER INSTITUTE   (Netherlands)

^c VU UNIVERSITY AMSTERDAM   (Netherlands)

^d LEIDEN UNIVERSITY   (Netherlands)

Author keywords

Alkaline phosphatase; Biochemical detection; Complex mixture screening; Dereplication; Drug discovery; Enzymatic assay; MDCC PBP; Phosphate

Indexed keywords

ALKALINITY; CHEMICAL REACTIONS; CONTROLLED DRUG DELIVERY; DRUG PRODUCTS; FLUORESCENCE; FLUOROPHORES; MASS SPECTROMETRY; MIXTURES; PHOSPHATASES; PHOSPHATES; PLANT EXTRACTS; PROBES; REMOVAL; TARGETED DRUG DELIVERY;

ALKALINE PHOSPHATASE; BIOCHEMICAL DETECTION; COMPLEX MIXTURE; DEREPLICATION; DRUG DISCOVERY; ENZYMATIC ASSAY; MDCC-PBP;

LIQUID CHROMATOGRAPHY;

ALKALINE PHOSPHATASE; ENZYME INHIBITOR; FLUORESCENT DYE; NATURAL PRODUCT; PHOSPHATE; PLANT EXTRACT; REAGENT; SOLVENT; TETRAMISOLE;

ANALYTIC METHOD; ARTICLE; CALIBRATION; DRUG DETERMINATION; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME MECHANISM; ENZYME SUBSTRATE; FLUORESCENCE; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; PHOSPHATE METABOLISM; PRIORITY JOURNAL;

EID: 0037402902     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0003-2697(03)00038-1     Document Type: Article

References (19)

1. Strege M.A. High-performance liquid chromatographic-electrospray ionization mass spectrometric analyses for the integration of natural products with modern high-throughput screening. J. Chromatogr. B. 725:1999;67-78.
2. Irth H., Oosterkamp A.J., van der Welle W., Tjaden U.R., van der Greef J. On-line immunochemical detection in liquid chromatography using fluorescein-labelled antibodies. J. Chromatogr. 633:1993;65-72.
3. Schobel U., Frenay M., van Elswijk D.A., McAndrews J.M., Long K.R., Olson L.M., Bobzin S.C., Irth H. High resolution screening of plant natural product extracts for estrogen receptor α and β binding activity using an online HPLC-MS biochemical detection system. J. Biomol. Screen. 6:2001;291-303.
4. Drews J. Drug discovery: a historical perspective. Science. 287:2000;1960-1964.
5. Missiaen L., Wuytack F., Kanmura Y., Van Belle H., Wynants J., Minten J., Casteels R. Measurement of microsomal ATPase activities: a comparison between the inorganic phosphate-release assay and the NADH-coupled enzyme assay. Biochim. Biophys. Acta. 990:1989;40-44.
6. Itaya K., Ui M. A new micromethod for the colorimetric determination of inorganic phosphate. Clin. Chim. Acta. 14:1966;361-366.
7. Fiske C.H., Subbarow Y. J. Biol. Chem. 66:1925;375-400.
8. Kirkbright G.F., Narayanaswamy R., West T.S. The spectrofluorimetric determination of orthophosphate as quinine molybdophosphate. Analyst. 97:1972;174-181.
9. Sebbon B., Fynn G.H. Orthophosphate analysis by the Fiske-Subbarow method and interference by adenosine phosphates and pyrophosphate at variable acid pH. Anal. Biochem. 56:1973;566-570.
10. Webb M.R. A continuous spectrophotometric assay for inorganic phosphate and for measuring phosphate release kinetics in biological systems. Proc. Natl. Acad. Sci. USA. 89:1992;4884-4887.
11. Ungerer J.P., Oosthuizen M.H., Bissbort S.H. An enzymatic assay of inorganic phosphate in serum using nucleoside phosphorylase and xanthine oxidase. Clin. Chim. Acta. 223:1993;149-157.
12. Tedokon M., Suzuki K., Kayamori Y., Fujita S., Katayama Y. Enzymatic assay of inorganic phosphate with use of sucrose phosphorylase and phosphoglucomutase. Clin. Chem. 38:1992;512-515.
13. Ellen Chan L.P., Swaminathan R. Adenosine triphosphate interferes with phosphate determination. Clin. Chem. 32:1986;1981-1982.
14. Tashima Y. Removal of protein interference in the Fiske-Subbarow method by sodium dodecyl sulfate. Anal. Biochem. 69:1975;410-414.
15. Hwang K.J. Interference of ATP and acidity in the determination of inorganic phosphate by the Fiske and Subbarow method. Anal. Biochem. 75:1976;40-44.
16. v.d Linden A.H., Meiring H.D., ten Bosch J.J., Arends J. Inorganic phosphate assay with the ascorbic acid or stannous chloride method: influence of fluoride. Caries Res. 20:1986;17-23.
17. Brune M., Hunter J.L., Corrie J.E.T., Webb M.R. Direct, real-time measurement of rapid inorganic phosphate release using a novel fluorescent probe and its application to actomyosin subfragment 1 ATPase. Biochemistry. 33:1994;8262-8271.
18. Brune M., Hunter J.L., Howell S.A., Martin S.R., Hazlett Th.L., Corrie J.E.T., Webb M.R. Mechanism of inorganic phosphate interaction with phosphate binding protein from Escherichia coli. Biochemistry. 37:1998;10370-10380.
19. Lente G., Magalhães M.E.A., Fábián I. Kinetics and mechanisms of complex formation reactions in the iron(III)-phosphate ion system at large iron(III) excess. Formation of a tetranulcear complex. Inorg. Chem. 39:2000;1950-1954.