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Volumn 379, Issue 2, 2004, Pages 367-374

Expression of YAP4 in Saccharomyces cerevisiae under osmotic stress

Author keywords

HOG (high osmolarity glycerol) pathway; Msn2p; Stress response element (STRE); YAP4 (CIN5); Yeast activator protein (Yap1p)

Indexed keywords

ENZYMES; GENES; MUTAGENESIS; PROTEINS; SENSITIVITY ANALYSIS; STRESS ANALYSIS; YEAST;

EID: 2342643434     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/BJ20031127     Document Type: Article
Times cited : (30)

References (49)
  • 3
    • 0035844224 scopus 로고    scopus 로고
    • Transcript expression in Saccharomyces cerevisiae at high salinity
    • Yale, J. and Bohnert, H. J. (2001) Transcript expression in Saccharomyces cerevisiae at high salinity. J. Biol. Chem. 276, 15996-16007
    • (2001) J. Biol. Chem. , vol.276 , pp. 15996-16007
    • Yale, J.1    Bohnert, H.J.2
  • 4
    • 2342476913 scopus 로고    scopus 로고
    • The osmotic stress response of Saccharomyces cerevisiae
    • Hohmann, S. and Mager, W. H., eds. Springer-Verlag, Berlin, Heidelberg, New York
    • Tamás, J. M. and Hohmann, S. (2003) The osmotic stress response of Saccharomyces cerevisiae. In Yeast Stress Responses, Topics in Current Genetics, Vol. 1. (Hohmann, S. and Mager, W. H., eds.) pp. 121-200, Springer-Verlag, Berlin, Heidelberg, New York
    • (2003) Yeast Stress Responses, Topics in Current Genetics , vol.1 , pp. 121-200
    • Tamás, J.M.1    Hohmann, S.2
  • 5
    • 0036282743 scopus 로고    scopus 로고
    • Osmotic stress signaling and osmoadaptation in yeasts
    • Hohmann, S. (2002) Osmotic stress signaling and osmoadaptation in yeasts. Microbiol. Mol. Biol. Rev. 66, 300-372
    • (2002) Microbiol. Mol. Biol. Rev. , vol.66 , pp. 300-372
    • Hohmann, S.1
  • 6
    • 0034708436 scopus 로고    scopus 로고
    • The transcriptional response of Saccharomyces cerevisiae to osmotic shock. Hot1p and Msn2p/Msn4p are required for the induction of subsets of high osmolarity glycerol pathway-dependent genes
    • Rep, M., Krantz, M., Thevelein, J. M. and Hohmann, S. (2000) The transcriptional response of Saccharomyces cerevisiae to osmotic shock. Hot1p and Msn2p/Msn4p are required for the induction of subsets of high osmolarity glycerol pathway-dependent genes. J. Biol. Chem. 275, 8290-8300
    • (2000) J. Biol. Chem. , vol.275 , pp. 8290-8300
    • Rep, M.1    Krantz, M.2    Thevelein, J.M.3    Hohmann, S.4
  • 8
    • 0032814143 scopus 로고    scopus 로고
    • Osmotic stress-induced gene expression in Saccharomyces cerevisiae requires Msn1p and the novel nuclear factor Hot1p
    • Rep, M., Reiser, V., Gartner, U., Thevelein, J. M., Hohmann, S., Ammerer, G. and Ruis, H. (1999) Osmotic stress-induced gene expression in Saccharomyces cerevisiae requires Msn1p and the novel nuclear factor Hot1p. Mol. Cell. Biol. 19, 5474-5485
    • (1999) Mol. Cell. Biol. , vol.19 , pp. 5474-5485
    • Rep, M.1    Reiser, V.2    Gartner, U.3    Thevelein, J.M.4    Hohmann, S.5    Ammerer, G.6    Ruis, H.7
  • 9
    • 0034747684 scopus 로고    scopus 로고
    • The Sko1p repressorand Gcn4p activator antagonistically modulate stress-regulated transcription in Saccharomyces cerevisiae
    • Pascual-Ahuir, A., Serrano, R. and Proft, M. (2001) The Sko1p repressorand Gcn4p activator antagonistically modulate stress-regulated transcription in Saccharomyces cerevisiae. Mol. Cell. Biol. 21, 16-25
    • (2001) Mol. Cell. Biol. , vol.21 , pp. 16-25
    • Pascual-Ahuir, A.1    Serrano, R.2    Proft, M.3
  • 10
    • 0035813146 scopus 로고    scopus 로고
    • Multiple levels of control regulate the yeast cAMP-response element-binding protein repressor Sko1p in response to stress
    • Pascual-Ahuir, A., Posas, F., Serrano, R. and Proft, M. (2001) Multiple levels of control regulate the yeast cAMP-response element-binding protein repressor Sko1p in response to stress. J. Biol. Chem. 276, 37373-37378
    • (2001) J. Biol. Chem. , vol.276 , pp. 37373-37378
    • Pascual-Ahuir, A.1    Posas, F.2    Serrano, R.3    Proft, M.4
  • 11
    • 0029879360 scopus 로고    scopus 로고
    • The Saccharomyces cerevisiae zinc finger proteins Msn2p and Msn4p are required for transcriptional induction through the stress-response element (STRE)
    • Martínez-Pastor, M. T., Marchler, G., Schüller, C., Marchler-Bauer, A., Ruis, H. and Estruch, F. (1996) The Saccharomyces cerevisiae zinc finger proteins Msn2p and Msn4p are required for transcriptional induction through the stress-response element (STRE). EMBO J. 15, 2227-2235
    • (1996) EMBO J. , vol.15 , pp. 2227-2235
    • Martínez-Pastor, M.T.1    Marchler, G.2    Schüller, C.3    Marchler-Bauer, A.4    Ruis, H.5    Estruch, F.6
  • 12
    • 0027156915 scopus 로고
    • A Saccharomyces cerevisiae UAS element controlled by protein kinase A activates transcription in response to a variety of stress conditions
    • Marchler, G., Schuller, C., Adam, G. and Ruis, H. (1993) A Saccharomyces cerevisiae UAS element controlled by protein kinase A activates transcription in response to a variety of stress conditions. EMBO J. 12, 1997-2003
    • (1993) EMBO J. , vol.12 , pp. 1997-2003
    • Marchler, G.1    Schuller, C.2    Adam, G.3    Ruis, H.4
  • 13
    • 0025862421 scopus 로고
    • Heat shock factor-independent heat control of transcription of the CTT1 gene encoding the cytosolic catalase T of Saccharomyces cerevisiae
    • Wieser, R., Adam, G., Wagner, A., Schuller, C., Marchler, G., Ruis, H., Krawiec, Z. and Bilinski (1991) Heat shock factor-independent heat control of transcription of the CTT1 gene encoding the cytosolic catalase T of Saccharomyces cerevisiae. J. Biol. Chem. 266, 12406-12411
    • (1991) J. Biol. Chem. , vol.266 , pp. 12406-12411
    • Wieser, R.1    Adam, G.2    Wagner, A.3    Schuller, C.4    Marchler, G.5    Ruis, H.6    Krawiec, Z.7    Bilinski8
  • 14
    • 0027441585 scopus 로고
    • Identification of cis- and trans-components of a novel heat shock stress regulatory pathway in Saccharomyces cerevisiae
    • Kobayashi, N. and McEntee, K. (1993) Identification of cis- and trans-components of a novel heat shock stress regulatory pathway in Saccharomyces cerevisiae. Mol. Cell. Biol. 13, 248-256
    • (1993) Mol. Cell. Biol. , vol.13 , pp. 248-256
    • Kobayashi, N.1    McEntee, K.2
  • 15
    • 0022555843 scopus 로고
    • The heat-shock response
    • Lindquist, S. (1986) The heat-shock response. Annu. Rev. Biochem. 55, 1151-1191
    • (1986) Annu. Rev. Biochem. , vol.55 , pp. 1151-1191
    • Lindquist, S.1
  • 16
    • 0032500690 scopus 로고    scopus 로고
    • Transcriptional factor mutations reveal regulatory complexities of heat shock and newly identified stress genes in Saccharomyces cerevisiae
    • Treger, J. M., Schmitt, A. P., Simon, J. R. and McEntee, K. (1998) Transcriptional factor mutations reveal regulatory complexities of heat shock and newly identified stress genes in Saccharomyces cerevisiae. J. Biol. Chem. 273, 26875-26879
    • (1998) J. Biol. Chem. , vol.273 , pp. 26875-26879
    • Treger, J.M.1    Schmitt, A.P.2    Simon, J.R.3    McEntee, K.4
  • 17
    • 0035064786 scopus 로고    scopus 로고
    • Hsf1p and Msn2/4p cooperate in the expression of Saccharomyces cerevisiae genes HSP26 and HSP104 in a gene- and stress-type-dependent manner
    • Amorós, M. and Estruch, F. (2001) Hsf1p and Msn2/4p cooperate in the expression of Saccharomyces cerevisiae genes HSP26 and HSP104 in a gene- and stress-type-dependent manner. Mol. Microbiol. 39, 1523-1532
    • (2001) Mol. Microbiol. , vol.39 , pp. 1523-1532
    • Amorós, M.1    Estruch, F.2
  • 18
    • 0036231909 scopus 로고    scopus 로고
    • HSF and Msn2/4p can exclusively or cooperatively activate the yeast HSP104 gene
    • Grably, M. R., Stanhill, A., Tell, O. and Engelberg, D. (2002) HSF and Msn2/4p can exclusively or cooperatively activate the yeast HSP104 gene. Mol. Microbiol. 44, 21-35
    • (2002) Mol. Microbiol. , vol.44 , pp. 21-35
    • Grably, M.R.1    Stanhill, A.2    Tell, O.3    Engelberg, D.4
  • 19
    • 0030712874 scopus 로고    scopus 로고
    • Yap, a novel family of eight bZIP proteins in Saccharomyces cerevisiae with distinct biological functions
    • Fernandes, L., Rodrigues-Pousada, C. and Struhl, K. (1997) Yap, a novel family of eight bZIP proteins in Saccharomyces cerevisiae with distinct biological functions. Mol. Cell. Biol. 17, 6982-6993
    • (1997) Mol. Cell. Biol. , vol.17 , pp. 6982-6993
    • Fernandes, L.1    Rodrigues-Pousada, C.2    Struhl, K.3
  • 20
    • 0025174216 scopus 로고
    • Chromosome instability mutants of Saccharomyces cerevisiae that are defective in microtubule-mediated processes
    • Hoyt, M. A., Stearns, T. and Botstein, D. (1990) Chromosome instability mutants of Saccharomyces cerevisiae that are defective in microtubule-mediated processes. Mol. Cell. Biol. 10, 223-234
    • (1990) Mol. Cell. Biol. , vol.10 , pp. 223-234
    • Hoyt, M.A.1    Stearns, T.2    Botstein, D.3
  • 22
    • 0031971250 scopus 로고    scopus 로고
    • Identification of Saccharomyces cerevisiae genes conferring resistance to quinoline ring-containing antimalarial drugs
    • Delling, U., Raymond, M., and Schurr, E. (1998) Identification of Saccharomyces cerevisiae genes conferring resistance to quinoline ring-containing antimalarial drugs. Antimicrob. Agents Chemother. 42, 1034-1041
    • (1998) Antimicrob. Agents Chemother. , vol.42 , pp. 1034-1041
    • Delling, U.1    Raymond, M.2    Schurr, E.3
  • 23
    • 0035126499 scopus 로고    scopus 로고
    • Two nuclear proteins, Cin5 and Ydr259c, confer resistance to cisplatin in Saccharomyces cerevisiae
    • Furuchi, T., Ishikawa, H., Miura, N., Ishizuka, M., Kajita, K., Kuge, S. and Naganuma, A. (2001) Two nuclear proteins, Cin5 and Ydr259c, confer resistance to cisplatin in Saccharomyces cerevisiae. Mol. Pharmacol. 59, 470-474
    • (2001) Mol. Pharmacol. , vol.59 , pp. 470-474
    • Furuchi, T.1    Ishikawa, H.2    Miura, N.3    Ishizuka, M.4    Kajita, K.5    Kuge, S.6    Naganuma, A.7
  • 24
    • 0028794516 scopus 로고
    • Construction of a set of convenient Saccharomyces cerevisiae strains that are isogenic to S288C
    • Winston, F., Dollard, C. and Ricupero-Hovasse, S. L. (1995) Construction of a set of convenient Saccharomyces cerevisiae strains that are isogenic to S288C. Yeast 11, 53-55.
    • (1995) Yeast , vol.11 , pp. 53-55
    • Winston, F.1    Dollard, C.2    Ricupero-Hovasse, S.L.3
  • 25
    • 0024977417 scopus 로고
    • Elevated recombination rates in the transcriptionally active DNA
    • Thomas, B. J. and Rothstein, R. G. (1989) Elevated recombination rates in the transcriptionally active DNA. Cell 56, 619-630
    • (1989) Cell , vol.56 , pp. 619-630
    • Thomas, B.J.1    Rothstein, R.G.2
  • 26
    • 0029994841 scopus 로고    scopus 로고
    • A new efficient gene disruption cassette for repeated use in budding yeast
    • Güldener, U., Heck, S., Fiedler, T., Beinhauer, J. and Hegemann, J. H. (1996) A new efficient gene disruption cassette for repeated use in budding yeast. Nucleic Acids Res. 24, 2519-2524
    • (1996) Nucleic Acids Res. , vol.24 , pp. 2519-2524
    • Güldener, U.1    Heck, S.2    Fiedler, T.3    Beinhauer, J.4    Hegemann, J.H.5
  • 29
    • 0028954118 scopus 로고
    • Studies on the transformation of intact yeast cells by the LiAc/ssDNA/PEG procedure
    • Gietz, R. D., Schiestl, R. H., Willems, A. R. and Woods, R. A. (1995) Studies on the transformation of intact yeast cells by the LiAc/ssDNA/PEG procedure. Yeast 11, 355-360
    • (1995) Yeast , vol.11 , pp. 355-360
    • Gietz, R.D.1    Schiestl, R.H.2    Willems, A.R.3    Woods, R.A.4
  • 30
    • 0023034916 scopus 로고
    • Yeast shuttle and integrative vectors with multiple cloning sites suitable for construction of Lac fusions
    • Myers, A. M., Tzagoloff, A., Kinney, D. M. and Lusty, C. J. (1986) Yeast shuttle and integrative vectors with multiple cloning sites suitable for construction of Lac fusions. Gene 45, 299-310
    • (1986) Gene , vol.45 , pp. 299-310
    • Myers, A.M.1    Tzagoloff, A.2    Kinney, D.M.3    Lusty, C.J.4
  • 31
    • 0024266139 scopus 로고
    • New yeast-E. coli shuttle vectors constructed with in vitro mutagenizad yeast genes lacking six base pair restriction sites
    • Gietz, R. D. and Sugino, A. (1988) New yeast-E. coli shuttle vectors constructed with in vitro mutagenizad yeast genes lacking six base pair restriction sites. Gene 74, 527-534
    • (1988) Gene , vol.74 , pp. 527-534
    • Gietz, R.D.1    Sugino, A.2
  • 32
    • 0024669291 scopus 로고
    • A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae
    • Sikorski, R. S. and Hieter, P. (1989) A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122, 19-27
    • (1989) Genetics , vol.122 , pp. 19-27
    • Sikorski, R.S.1    Hieter, P.2
  • 33
    • 0032828715 scopus 로고    scopus 로고
    • A generic protein purification method for protein complex characterization and proteome exploration
    • Rigaut, G., Sherchenko, A., Rutz, B., Wilm, M., Mann, M. and Seraphin, B. (1999) A generic protein purification method for protein complex characterization and proteome exploration. Nat. Biotechnol. 17, 1030-1032
    • (1999) Nat. Biotechnol. , vol.17 , pp. 1030-1032
    • Rigaut, G.1    Sherchenko, A.2    Rutz, B.3    Wilm, M.4    Mann, M.5    Seraphin, B.6
  • 34
    • 0017184389 scopus 로고
    • A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding
    • Bradford, M. M. (1976). A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254
    • (1976) Anal. Biochem. , vol.72 , pp. 248-254
    • Bradford, M.M.1
  • 35
    • 0034597012 scopus 로고    scopus 로고
    • 2 sensing through oxidation of the Yap1 transcription factor
    • 2 sensing through oxidation of the Yap1 transcription factor. EMBO J. 19, 5157-5166
    • (2000) EMBO J. , vol.19 , pp. 5157-5166
    • Delaunay, A.1    Isnard, A.D.2    Toledano, M.B.3
  • 36
    • 0033025517 scopus 로고    scopus 로고
    • Different signalling pathways contribute to the control of GPD1 gene expression by osmotic stress in Saccharomyces cerevisiae
    • Rep, M., Albertyn, J., Thevelein, J. M., Prior, B. A. and Hohmann, S. (1999) Different signalling pathways contribute to the control of GPD1 gene expression by osmotic stress in Saccharomyces cerevisiae. Microbiology 145, 715-727
    • (1999) Microbiology , vol.145 , pp. 715-727
    • Rep, M.1    Albertyn, J.2    Thevelein, J.M.3    Prior, B.A.4    Hohmann, S.5
  • 37
    • 0033923859 scopus 로고    scopus 로고
    • The control of intracellular glycerol in Saccharomyces cerevisiae influences osmotic stress response and resistance to increased temperature
    • Siderius, M., Van Wuytswinkel, O., Reijenga, K. A., Kelders, M. and Mager, W. H. (2000) The control of intracellular glycerol in Saccharomyces cerevisiae influences osmotic stress response and resistance to increased temperature. Mol. Microbiol. 36, 1381-1390
    • (2000) Mol. Microbiol. , vol.36 , pp. 1381-1390
    • Siderius, M.1    Van Wuytswinkel, O.2    Reijenga, K.A.3    Kelders, M.4    Mager, W.H.5
  • 38
    • 0029050236 scopus 로고
    • A genomic locus in Saccharomyces cerevisiae with four genes up-regulated by osmotic stress
    • Miralles, V. J. and Serrano, R. (1995) A genomic locus in Saccharomyces cerevisiae with four genes up-regulated by osmotic stress. Mol. Microbiol. 17, 653-662
    • (1995) Mol. Microbiol. , vol.17 , pp. 653-662
    • Miralles, V.J.1    Serrano, R.2
  • 39
    • 0032035043 scopus 로고    scopus 로고
    • Signal transduction by MAP kinase cascades in budding yeast
    • Posas, F., Takekawa, M. and Saito, H. (1998) Signal transduction by MAP kinase cascades in budding yeast. Curr. Opin. Microbiol. 1, 175-182
    • (1998) Curr. Opin. Microbiol. , vol.1 , pp. 175-182
    • Posas, F.1    Takekawa, M.2    Saito, H.3
  • 40
    • 0027531645 scopus 로고
    • An osmosensing signal transduction pathway in yeast
    • Washington, D.C.
    • Brewster, J. L., de Valoir, T., Dwyer, N. D., Winter, E. and Gustin, M. C. (1993) An osmosensing signal transduction pathway in yeast. Science (Washington, D.C.) 19, 1760-1763
    • (1993) Science , vol.19 , pp. 1760-1763
    • Brewster, J.L.1    De Valoir, T.2    Dwyer, N.D.3    Winter, E.4    Gustin, M.C.5
  • 41
    • 0037214352 scopus 로고    scopus 로고
    • Targeting the MEF2-like transcription factor Smp1 by the stress-activated Hog1 mitogen-activated protein kinase
    • de Nadal, E., Casadome, L. and Posas, F. (2003) Targeting the MEF2-like transcription factor Smp1 by the stress-activated Hog1 mitogen-activated protein kinase. Mol. Cell. Biol. 23, 229-237
    • (2003) Mol. Cell. Biol. , vol.23 , pp. 229-237
    • De Nadal, E.1    Casadome, L.2    Posas, F.3
  • 42
    • 0030003064 scopus 로고    scopus 로고
    • Msn2p, a zinc finger DNA-binding protein, is the transcriptional activator of the multistress response in Saccharomyces cerevisiae
    • Schmitt, A. P. and McEntee, K. (1996) Msn2p, a zinc finger DNA-binding protein, is the transcriptional activator of the multistress response in Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. U.S.A. 93, 5777-5782
    • (1996) Proc. Natl. Acad. Sci. U.S.A. , vol.93 , pp. 5777-5782
    • Schmitt, A.P.1    McEntee, K.2
  • 43
    • 0033037610 scopus 로고    scopus 로고
    • The heat-shock response in yeast: Differential regulations and contributions of the Msn2p/Msn4p and Hsf1p regulons
    • Boy-Marcotte, E., Lagniel, G., Perrot, M., Bussereau, F., Boudsocq, A., Jacquet M. and Labarre, J. (1999) The heat-shock response in yeast: differential regulations and contributions of the Msn2p/Msn4p and Hsf1p regulons. Mol. Microbiol. 33, 274-283
    • (1999) Mol. Microbiol. , vol.33 , pp. 274-283
    • Boy-Marcotte, E.1    Lagniel, G.2    Perrot, M.3    Bussereau, F.4    Boudsocq, A.5    Jacquet, M.6    Labarre, J.7
  • 45
    • 0029895124 scopus 로고    scopus 로고
    • Characterization of the NHA1 gene encoding a Na+/H+-antiporter of the yeast Saccharomyces cerevisiae
    • Prior, C., Potier, S., Souciet, J. L. and Sychrova, H. (1996) Characterization of the NHA1 gene encoding a Na+/H+-antiporter of the yeast Saccharomyces cerevisiae. FEBS Lett. 387, 89-93
    • (1996) FEBS Lett. , vol.387 , pp. 89-93
    • Prior, C.1    Potier, S.2    Souciet, J.L.3    Sychrova, H.4
  • 46
    • 0030590901 scopus 로고    scopus 로고
    • The involvement of the Saccharomyces cerevisiae multidrug resistance transporters Pdr5p and Snq2p in cation resistance
    • Miyahara, K., Mizunuma, M., Hirata, D., Tsuchiya, E. and Miyakawa, T. (1996) The involvement of the Saccharomyces cerevisiae multidrug resistance transporters Pdr5p and Snq2p in cation resistance. FEBS Lett. 399, 317-320
    • (1996) FEBS Lett. , vol.399 , pp. 317-320
    • Miyahara, K.1    Mizunuma, M.2    Hirata, D.3    Tsuchiya, E.4    Miyakawa, T.5
  • 47
    • 0024042922 scopus 로고
    • TRK1 encodes a plasma membrane protein required for high-affinity potassium transport in Saccharomyces cerevisiae
    • Gaber, R. F., Styles, C. A. and Fink, G. R. (1988) TRK1 encodes a plasma membrane protein required for high-affinity potassium transport in Saccharomyces cerevisiae. Mol. Cell. Biol. 8, 2848-2859
    • (1988) Mol. Cell. Biol. , vol.8 , pp. 2848-2859
    • Gaber, R.F.1    Styles, C.A.2    Fink, G.R.3
  • 48
    • 0141643119 scopus 로고    scopus 로고
    • Coordinate expression of NADPH-dependent flavin reductase, Fre-1, and Hint-related 7meGMP-directed hydrolase, DCS-1
    • Kwasnicka, D. A., Krakowiak, A., Thacker, C., Brenner, C. and Vincent, S. R. (2003) Coordinate expression of NADPH-dependent flavin reductase, Fre-1, and Hint-related 7meGMP-directed hydrolase, DCS-1. J. Biol. Chem. 278, 39051-39058
    • (2003) J. Biol. Chem. , vol.278 , pp. 39051-39058
    • Kwasnicka, D.A.1    Krakowiak, A.2    Thacker, C.3    Brenner, C.4    Vincent, S.R.5
  • 49
    • 9444298995 scopus 로고    scopus 로고
    • In silico and in vivo analysis reveal a novel gene in Saccharomyces cerevisiae trehalose metabolism
    • De Mesquita, J. F., Panek, A. D. and de Araujo, P. S. (2003) In silico and in vivo analysis reveal a novel gene in Saccharomyces cerevisiae trehalose metabolism. BMC Genomics 4, 45-50
    • (2003) BMC Genomics , vol.4 , pp. 45-50
    • De Mesquita, J.F.1    Panek, A.D.2    De Araujo, P.S.3


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