A simplified HPLC method for total isoflavones in soy products

Food Chemistry

Volumn 87, Issue 2, 2004, Pages 297-305

  Peñalvo, José L ^a   Nurmi, Tarja ^a,b   Adlercreutz, Herman ^a  

^a UNIVERSITY OF HELSINKI   (Finland)

^b UNIVERSITY OF EASTERN FINLAND   (Finland)

Author keywords

Coulometric detection; HPLC; Isoflavones; Soy

Indexed keywords

6'' O ACETYLDAIDZIN; 6'' O ACETYLGENISTIN; 6'' O ACETYLGLYCITIN; 6'' O MALONYLDAIDZIN; 6'' O MALONYLGENISTIN; 6'' O MALONYLGLYCITIN; 7 O GLUCOSIDE; ACETYLGLUCOSIDE; ALCOHOL; DAIDZIN; GENISTEIN; GENISTIN; GLUCOSIDE; GLYCITEIN; GLYCITIN; HYDROCHLORIC ACID; ISOFLAVONE DERIVATIVE; MALONYLGLUCOSIDE; PHYTOESTROGEN; UNCLASSIFIED DRUG;

ARTICLE; COULOMETRY; EXTRACTION; FOOD ANALYSIS; FOOD COMPOSITION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HYDROLYSIS; SOYBEAN;

GLYCINE MAX;

EID: 2342636477     PISSN: 03088146     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.foodchem.2003.12.028     Document Type: Article

References (23)

1. Adlercreutz H., Goldin B.R., Gorbach S.L., Höckerstedt K.A.V., Watanabe S., Hämäläinen E.K., Markkanen M.H., Mäkelä T.H., Wähälä K.T., Hase T.A., Fotsis T. Soybean phytoestrogen intake and cancer risk. Journal of Nutrition. 125:1995;757S-770S
2. Allred C.D., Ju Y.H., Allred K.F., Chang J., Helferich W.G. Dietary genistin stimulates growth of estrogen-dependent breast cancer tumors similar to that observed with genistein. Carcinogenesis. 22(10):2001;1667-1673
3. Barnes S. Evolution of the health benefits of soy isoflavones. Proceedings of the Society for Experimental Biology and Medicine. 217(3):1998;386-392
4. Barnes S., Kirk M., Coward L. Isoflavones and their conjugates in soy foods - extraction conditions and analysis by HPLC mass spectrometry. Journal Agricultural Food Chemistry. 42(11):1994;2466-2474
5. Bowey E., Adlercreutz H., Rowland I. Metabolism of isoflavones and lignans by the gut microflora: a study in germ-free and human flora associated rats. Food and Chemical Toxicology. 41(5):2003;631-636
6. Coward L., Barnes N.C., Setchell K.D.R., Barnes S. Genistein, daidzein and their b-glycoside conjugates: antitumor isoflavones in soybean foods from american and asian diets. Journal of Agricultural and Food Chemistry. 41(11):1993;1961-1967
7. Franke A.A., Custer L.J., Cerna C.M., Narala K. Rapid HPLC analysis of dietary phytoestrogens from legumes and from human urine. Proceedings of the Society for Experimental Biology and Medicine. 208(1):1995;18-26
8. Franke A., Hankin J.H., Yu M.C., Maskarinec G., Low S., Custer L.J. Isoflavone levels in soy foods consumed by multiethnic populations in Singapore and Hawaii. Journal of Agricultural and Food Chemistry. 47(3):1999;977-986
9. Griffith A.P., Collison M.W. Improved methods for the extraction of isoflavones from soy-containing foods and nutritional supplements by reversed-phase liquid chromatography and liquid chromatography - mass spectrometry. Journal of Chromatography A. 913:2001;397-413
10. Izumi T., Piskula M.K., Osawa S., Obata A., Tobe K., Saito M., Kataoka S., Kubota Y., Kikuchi M. Soy isoflavone aglycones are absorbed faster and in higher amounts than their glucosides in humans. Journal of Nutrition. 130(7):2000;1695-1699
11. Kapiotis S., Hermann M., Held I., Seelos C., Ehringer H., Gmeiner B.M.K. Genistein, the dietary-derived angiogenesis inhibitor, prevents LDL oxidation and protects endothelial cells from damage by atherogenic LDL. Arteriosclerosis, Thrombosis and Vascular Biology. 17(11):1997;2868-2874
12. Klump S.P.A., McDonald J.L., Ballam J.M. Determination of isoflavones in soy selected foods containing soy by extraction, saponification, and liquid chromatography: collaborative study. Journal of AOAC International. 84(6):2001;1865-1883
13. Kurzer M.S. Hormonal effects of soy isoflavones: studies in premenopausal and postmenopausal women. Journal of Nutrition. 130:2000;660S-661S
14. Liggins J., Bluck L.J.C., Coward W.A., Bingham S.A. Extraction and quantification of daidzein and genistein in food. Analytical Biochemistry. 264(1):1998;1-7
15. Mazur W., Fotsis T., Wähälä K., Ojala S., Salakka A., Adlercreutz H. Isotope dilution gas chromatographic-mass spectrometric method for the determination of isoflavonoids, coumestrol, and lignans in food samples. Analytical Biochemistry. 233:1996;169-180
16. Murphy P.A. Separation of genistin, and coumestrol by gradient high-performance liquid chromatography, daizdin and their aglucones. Journal of Chromatography A. 211(1):1981;166-169
17. Naim M., Gestetner B., Zilkah S. Soybean isoflavones characterization, determination, and antifungal activity. Journal of Agricultural and Food Chemistry. 22(5):1974;806-810
18. Nurmi T., Mazur W., Heinonen S., Kokkonen J., Adlercreutz H. Isoflavone content of the soy based supplements. Journal of Pharmaceutical and Biomedical. 28:2002;1-11
19. Piskula M.K., Yamakoshi J., Iwai Y. Daidzein and genistein but not their glucosides are absorbed from the rat stomach. FEBS Letters. 447:1999;287-291
20. Setchell K.D.R., Brown N.M., Desai P., Zimmer-Nechemias L., Wolfe B.E., Brasher W.T., Kirschner A.S., Cassidy A., Heubi J.E. Bioavailabilty of pure isoflavones in healthy humans and analysis of commercial soy isoflavone supplements. Journal of Nutrition. 131:2001;1362S-1375S
21. Setchell D.R.K., Brown N.M., Zimmer-Nechemias L., Brashear W.T., Wolfe B.E., Kirschner A.S., Heubi J.E. Evidence for lack of absorption of soy isoflavone glycosides in humans, supporting the crucial role of intestinal metabolism for bioavailability. American Journal of Clinical Nutrition. 76:2002;447-453
22. Wang H.J., Murphy P.A. Isoflavone content in commercial soybean foods. Journal of Agricultural Food Chemistry. 42(8):1994;1666-1673
23. Wiseman H.C., Clarke D.B., Barnes K.A., Bowey E. Isoflavone aglycone and glucoconjugate of high- and low-soy U.K. foods used in nutritional studies. Journal of Agricultural Food Chemistry. 50(6):2002;1404-1410