X-ray structure of the R69D phosphatidylinositol-specific phospholipase C enzyme: Insight into the role of calcium and surrounding amino acids in active site geometry and catalysis

Biochemistry

Volumn 44, Issue 30, 2005, Pages 9980-9989

  Apiyo, David ^a   Zhao, Li ^b   Tsai, Ming Daw ^b   Selby, Thomas L ^a  

^a UNIVERSITY OF CENTRAL FLORIDA   (United States)

^b OHIO STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; CALCIUM; CATALYSIS; ESTERIFICATION; HYDROGEN BONDS; STEREOCHEMISTRY; SUBSTRATES; X RAY CRYSTALLOGRAPHY;

HYDROXYL GROUP; PHOSPHODIESTERASES; PHOSPHOLIPASE CS (PLC); X RAY STRUCTURE;

PHOSPHATES;

ARGININE; ASPARTIC ACID; CALCIUM ION; HYDROXYL GROUP; INOSITOL PHOSPHATE; METAL ION; PHOSPHATIDYLINOSITOL 4,5 BISPHOSPHATE PHOSPHODIESTERASE;

AMINO ACID SUBSTITUTION; ARTICLE; BACILLUS THURINGIENSIS; CATALYSIS; ENZYME ACTIVE SITE; ENZYME STABILITY; ENZYME STRUCTURE; ENZYME SUBSTRATE COMPLEX; EVOLUTION; GEOMETRY; HYDROGEN BOND; MOLECULAR WEIGHT; NONHUMAN; PRIORITY JOURNAL; PROTEIN FAMILY; STEREOCHEMISTRY; TRANSESTERIFICATION; X RAY ANALYSIS;

AMINO ACID SEQUENCE; ANIMALS; ARGININE; ASPARTIC ACID; BACILLUS CEREUS; BACILLUS THURINGIENSIS; BINDING SITES; CALCIUM; CATALYSIS; CRYSTALLIZATION; CRYSTALLOGRAPHY, X-RAY; HISTIDINE; HUMANS; INOSITOL 1,4,5-TRISPHOSPHATE; INOSITOL PHOSPHATES; MOLECULAR SEQUENCE DATA; PHOSPHATIDYLINOSITOL DIACYLGLYCEROL-LYASE; RATS; STEREOISOMERISM;

BACILLUS (BACTERIUM); MAMMALIA;

EID: 23044490267     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi047896v     Document Type: Article

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