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Volumn 17, Issue 1, 2002, Pages 15-18

Individual molecules of thermostable alkaline phosphatase support different catalytic rates at room temperature

Author keywords

Alkaline phosphatase; Single molecule assay; Thermus thermophilus

Indexed keywords

ASSAYS; CAPILLARY ELECTROPHORESIS; MOLECULES; PHOSPHATASES;

EID: 0036358360     PISSN: 15227235     EISSN: None     Source Type: Journal    
DOI: 10.1002/bio.672     Document Type: Article
Times cited : (17)

References (13)
  • 1
    • 0028934277 scopus 로고
    • Differences in the chemical reactivity of individual molecules of an enzyme
    • Xue Q and Yeung ES. Differences in the chemical reactivity of individual molecules of an enzyme. Nature 1995; 373: 681-683.
    • (1995) Nature , vol.373 , pp. 681-683
    • Xue, Q.1    Yeung, E.S.2
  • 2
    • 0007042628 scopus 로고    scopus 로고
    • Monitoring the reactions of single enzyme molecules and single metal ions
    • Tan W and Yeung ES. Monitoring the reactions of single enzyme molecules and single metal ions. Anal. Chem. 1997; 69: 4242-4248.
    • (1997) Anal. Chem. , vol.69 , pp. 4242-4248
    • Tan, W.1    Yeung, E.S.2
  • 3
    • 0030003076 scopus 로고    scopus 로고
    • Studies on single alkaline phosphatase molecules: Reaction rate and activation energy of a reaction catalyzed by a single molecule and the effect of thermal denaturation - The death of an enzyme
    • Craig DB, Arriaga EA, Wong JCY, Lu H and Dovichi NJ. Studies on single alkaline phosphatase molecules: reaction rate and activation energy of a reaction catalyzed by a single molecule and the effect of thermal denaturation - the death of an enzyme. J. Am. Chem. Soc. 1996; 118: 5245-5253.
    • (1996) J. Am. Chem. Soc. , vol.118 , pp. 5245-5253
    • Craig, D.B.1    Arriaga, E.A.2    Wong, J.C.Y.3    Lu, H.4    Dovichi, N.J.5
  • 5
    • 0032484096 scopus 로고    scopus 로고
    • Single-molecule enzymatic dynamics
    • Lu HP, Sun L and Xie XS. Single-molecule enzymatic dynamics. Science 1998; 282: 1877-1882.
    • (1998) Science , vol.282 , pp. 1877-1882
    • Lu, H.P.1    Sun, L.2    Xie, X.S.3
  • 6
    • 0032066823 scopus 로고    scopus 로고
    • E. coli β-galactosidase is heterogeneous with respect to the activity of individual molecules
    • Craig DB and Dovichi NJ. E. coli β-galactosidase is heterogeneous with respect to the activity of individual molecules. Can. J. Chem. 1998; 76: 623-626.
    • (1998) Can. J. Chem. , vol.76 , pp. 623-626
    • Craig, D.B.1    Dovichi, N.J.2
  • 8
    • 0034253211 scopus 로고    scopus 로고
    • Newly induced β-galactosidase molecules have a higher activity than the basally expressed enzyme
    • Craig DB and Hall T. Newly induced β-galactosidase molecules have a higher activity than the basally expressed enzyme. J. Clin. Laser Med. Surg. 2000; 18: 209-214.
    • (2000) J. Clin. Laser Med. Surg. , vol.18 , pp. 209-214
    • Craig, D.B.1    Hall, T.2
  • 9
    • 0033710974 scopus 로고    scopus 로고
    • E. coli β-galactosidase is heterogeneous with respect to a requirement for magnesium
    • Craig DB, Hall T and Goltz DM. E. coli β-galactosidase is heterogeneous with respect to a requirement for magnesium. BioMetals 2000; 13: 223-229.
    • (2000) BioMetals , vol.13 , pp. 223-229
    • Craig, D.B.1    Hall, T.2    Goltz, D.M.3
  • 10
    • 0028963577 scopus 로고
    • Fluorescence-based enzymatic assay by capillary electrophoresis laser-induced detection for the determination of a few β-galactosidase molecules
    • Craig D, Arriaga EA and Banks P et al. Fluorescence-based enzymatic assay by capillary electrophoresis laser-induced detection for the determination of a few β-galactosidase molecules. Anal. Biochem. 1995; 226: 147-153.
    • (1995) Anal. Biochem. , vol.226 , pp. 147-153
    • Craig, D.1    Arriaga, E.A.2    Banks, P.3
  • 11
    • 0028059166 scopus 로고
    • Yoctomolar detection limit by laser-induced fluorescence in capillary electrophoresis
    • Chen DY and Dovichi NJ. Yoctomolar detection limit by laser-induced fluorescence in capillary electrophoresis. J. Chromatogr. B 1994; 657: 265-269.
    • (1994) J. Chromatogr. B , vol.657 , pp. 265-269
    • Chen, D.Y.1    Dovichi, N.J.2
  • 12
    • 0000259568 scopus 로고    scopus 로고
    • Single-molecule detection in capillary electrophoresis: Molecular shot noise as a fundamental limit to chemical analysis
    • Chen DY and Dovichi NJ. Single-molecule detection in capillary electrophoresis: molecular shot noise as a fundamental limit to chemical analysis. Anal. Chem. 1996; 68: 690-696.
    • (1996) Anal. Chem. , vol.68 , pp. 690-696
    • Chen, D.Y.1    Dovichi, N.J.2
  • 13
    • 0034709430 scopus 로고    scopus 로고
    • Single molecules of highly purified bacterial alkaline phosphatase have identical activity
    • Polakowski R, Craig DB, Skelley A and Dovichi NJ. Single molecules of highly purified bacterial alkaline phosphatase have identical activity. J. Am. Chem. Soc. 2000; 122: 4853-4855.
    • (2000) J. Am. Chem. Soc. , vol.122 , pp. 4853-4855
    • Polakowski, R.1    Craig, D.B.2    Skelley, A.3    Dovichi, N.J.4


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.