siRNA produced by recombinant dicer mediates efficient gene silencing in islet cells

Annals of the New York Academy of Sciences

Volumn 1040, Issue , 2005, Pages 114-122

  Hägerkvist, Robert ^a   Mokhtari, Dariush ^a   Myers, Jason W ^b   Tengholm, Anders ^a   Welsh, Nils ^a  

^a UPPSALA UNIVERSITY   (Sweden)

^b STANFORD UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

c Abl; d siRNA; Gene silencing; Islet cells; Lipofection; Posttranscriptional gene silencing; Recombinant Dicer; RNAi; siRNA

Indexed keywords

ABELSON KINASE; DOUBLE STRANDED RNA; MESSENGER RNA; RECOMBINANT DICER; RECOMBINANT ENZYME; RIBONUCLEASE III; SMALL INTERFERING RNA; UNCLASSIFIED DRUG;

ANIMAL CELL; CONFERENCE PAPER; CONTROLLED STUDY; DIABETES MELLITUS; GENE EXPRESSION; GENE FUNCTION; GENE SILENCING; GENETIC TRANSFECTION; GENETIC VARIABILITY; MOUSE; NONHUMAN; PANCREAS ISLET CELL; RAT; RNA INTERFERENCE; RNA TRANSCRIPTION; RNA TRANSPORT;

EID: 22144439750     PISSN: 00778923     EISSN: None     Source Type: Book Series    
DOI: 10.1196/annals.1327.014     Document Type: Conference Paper

References (18)

1. HAMMOND, S.M., E. BERNSTEIN, D. BEACH & G.J. HANNON. 2000. An RNA-directed nuclease mediates post-transcriptional gene silencing in Drosophila cells. Nature 404: 293-296.
2. FIRE, A., S. XU, M.K. MONTGOMERY et al. 1998. Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature 391: 806-811.
3. ELBASHIR, S.M., J. HARBORTH, A. LENDECKEL et al. 2001. Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature 411: 494-498.
4. LEWIS, D.L., J.L. HAGSTROM, A.G. LOOMIS et al. 2002. Efficient delivery of siRNA for inhibition of gene expression in postnatal mice. Nat. Genet. 32: 107-108.
5. KHVOROVA, A., A. REYNOLDS & S.D. JAYASENA. 2003. Functional siRNA and miRNA exhibit strand bias. Cell 115: 209-216.
6. MYERS, J.W., J.T. JONES, T. MEYER & J.E. FERREL. 2003. Recombinant Dicer efficiently converts large dsRNAs into siRNAs suitable for gene silencing. Nat. Biotechnol. 21: 324-328.
7. PLASTERK, R.H.A. 2002. RNA silencing: the genome's immune system. Science 296: 1263-1265.
8. SANDLER, S., A. ANDERSSON & C. HELLERSTROM. 1987. Inhibitory effects of interleukin 1 on insulin biosynthesis and oxidative metabolism of isolated rat pancreatic islets. Endocrinology 121: 1424-1431.
9. LU, L., K. HOLMQVIST, M. CROSS & M. WELSH. 2002. Role of the Src homology 2 domain-containing protein Shb in murine brain endothelial cell proliferation and differentiation. Cell Growth Differ. 13: 141-148.
10. ERPEL, T., G. SUPERTI-FURGA & S.A. COURTNEIDGE. 1995. Mutational analysis of the Src SH3 domain: the same residues of the ligand binding surface are important for intra- and intermolecular interactions. EMBO J. 14: 963-975.
11. WELSH, N. 2000. Assessment of apoptosis and necrosis in isolated islets of Langerhans: methodological considerations. Curr. Top. Biochem. Res. 3: 189-200.
12. VAN ETTEN, R.A. 1999. Cycling, stressed-out and nervous: cellular functions of c-Abl. Trends Cell Biol. 9: 179-186.
13. WANG, J.Y. 2000. Regulation of cell death by the Abl tyrosine kinase. Oncogene 19: 5643-5650.
14. ITOH, Y., Y. KAWAMATA, M. HARADA et al. 2003. Free fatty acids regulate insulin secretion from pancreatic beta cells through GPR40. Nature 422: 173-176.
15. DA SILVA XAVIER, G., Q. QIAN, P.J. CULLEN & G.A. RUTTER. 2004. Distinct roles for insulin and insulin-like growth factor-1 receptors in pancreatic beta-cell glucose sensing revealed by RNA silencing. J. Biochem. 377: 149-158.
16. DEMETERCO, C. & F. LEVINE. 2001. Gene therapy for diabetes. Front. Biosci. 6: 175-191.
17. ZHDANOV, R.I., O.V. PODOBED & V.V. VLASSOV. 2002. Cationic lipid-DNA complexes-lipoplexes for gene transfer and therapy. Biochemistry 58: 53-64.
18. RUBINSON, D.A., C.P. DILLON, A.V. KWIATOWSKI et al. 2003. A lenti-virus-based system to functionally silence genes in primary mammalian cells, stem cells, and transgenic mice by RNA interference. Nat. Genet. 33: 401-406.