Contribution of major bacterial groups to bacterial biomass production (thymidine and leucine incorporation) in the Delaware estuary

Limnology and Oceanography

Volumn 48, Issue 1 I, 2003, Pages 168-178

  Cottrell, Matthew T ^a   Kirchman, David L ^a  

^a UNIVERSITY OF DELAWARE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIOPLANKTON; BIOLOGICAL PRODUCTION; BIOMASS; ESTUARY; SALINITY;

DELAWARE ESTUARY; UNITED STATES;

ALPHAPROTEOBACTERIA; BACTERIA (MICROORGANISMS); BETAPROTEOBACTERIA; CYTOPHAGA; GAMMAPROTEOBACTERIA; NEGIBACTERIA;

EID: 0037250698     PISSN: 00243590     EISSN: None     Source Type: Journal    
DOI: 10.4319/lo.2003.48.1.0168     Document Type: Article

References (38)

1. AMANN, R. I., B. J. BINDER, R. J. OLSON, S. W. CHISHOLM, R. DEVEREUX, AND D. A. STAHL. 1990. Combination of 16S rRNA-targeted oligonucleotide probes with flow cytometry for analyzing mixed microbial populations. Appl. Environ. Microbiol. 56: 1919-1925.
2. AZAM, F. 1998. Microbial control of oceanic carbon flux: The plot thickens. Science 280: 694-696.
3. BARER, M. R., AND A. ENTWISTLE. 1991. Confocal microscopy of surface labeled and cytoplasmically labeled bacteria immobilized by APS centrifugation. Lett. Appl. Microbiol. 13: 186-189.
4. BOUVIER, T. C., AND P. A. DEL GIORGIO. 2002. Compositional changes in free-living bacterial communities along a salinity gradient in two temperate estuaries. Limnol. Oceanogr. 47: 453-470.
5. CARMAN, K. R. 1993. Microautoradiographic detection of microbial activity, p. 397-404. In P. F. Kemp, B. F. Sherr, E. B. Sherr, and J. J. Cole [eds.], Handbook of methods in aquatic microbial ecology. Lewis.
6. COTTRELL, M. T., AND D. L. KIRCHMAN. 2000a. Natural assemblages of marine proteobacteria and members of the Cytophaga Flavobacter cluster consuming low- and high-molecular-weight dissolved organic matter. Appl. Environ. Microbiol. 66: 1692-1697.
7. _, AND _. 2000b. Community composition of marine bacterioplankton determined by 16S rRNA gene clone libraries and fluorescence in situ hybridization. Appl. Environ. Microbiol. 66: 5116-5122.
8. DEL GIORGIO, P. A., AND T. C. BOUVIER. 2002. Linking the physiologic and phylogenetic successions in free-living bacterial communities along an estuarine salinity gradient. Limnol. Oceanogr. 47: 471-486.
9. DOUGLAS, D. J., J. A. NOVITSKY, AND R. O. FOURNIER. 1987. Microautoradiography-based enumeration of bacteria with estimates of thymidine-specific growth and production rates. Mar. Ecol. Prog. Ser. 36: 91-99.
10. DUCKLOW, H. 2000. Bacterial production and biomass in the oceans, p. 85-120. In D. L. Kirchman [ed.], Microbial ecology of the oceans. Wiley.
11. EILERS, H., J. PERNTHALER, F. O. GLOCKNER, AND R. AMANN. 2000a. Culturability and in situ abundance of pelagic bacteria from the North Sea. Appl. Environ. Microbiol. 66: 3044-3051.
12. _, _, AND R. AMANN. 2000b. Succession of pelagic marine bacteria during enrichment: A close look at cultivation-induced shifts. Appl. Environ. Microbiol. 66: 4634-4640.
13. FUCHS, B. M., M. V. ZUBKOV, K. SAHM, P. H. BURKILL, AND R. AMANN. 2000. Changes in community composition during dilution cultures of marine bacterioplankton as assessed by flow cytometric and molecular biological techniques. Environ. Microbiol. 2: 191-201.
14. FUHRMAN, J. A. 1992. Bacterioplankton roles in cycling of organic matter: The microbial food web, p. 361-383. In P. G. Falkowski and A. D. Woodhead [eds.], Primary productivity and biogeochemical cycles in the Sea. Plenum Press.
15. _, AND F. AZAM. 1982. Thymidine incorporation as a measure of heterotrophic bacterioplankton production in marine surface waters - evaluation and field results. Mar. Biol. 66: 109-120.
16. GIOVANNONI, S., AND M. RAPPÉ. 2000. Evolution, diversity and molecular ecology of marine prokaryotes, p. 47-84. In D. L. Kirchman [ed.], Microbial ecology of the oceans. Wiley-Liss.
17. GLÖCKNER, F. O., B. M. FUCHS, AND R. AMANN. 1999. Bacterioplankton compositions of lakes and oceans: A first comparison based on fluorescence in situ hybridization. Appl. Environ. Microbiol. 65: 3721-3726.
18. KARNER, M., AND J. A. FUHRMAN. 1997. Determination of active marine bacterioplankton: A comparison of universal 16S rRNA probes, autoradiography, and nucleoid staining. Appl. Environ. Microbiol. 63: 1208-1213.
19. _, E. K'NEES, AND R. HODSON. 1985. Leucine incorporation and its potential as a measure of protein synthesis by bacteria in natural aquatic systems. Appl. Environ. Microbiol. 49: 599-607.
20. KIRCHMAN, D. L. 2002. The ecology of Cytophaga-flavobacteria in aquatic environments. FEMS Microbiol. Ecol. 39: 91-100.
21. _, B. MEON, M. T. COTTRELL, D. A. HUTCHINS, D. WEEKS, AND K. W. BRULAND. 2000. Carbon versus iron limitation of bacterial growth in the California upwelling regime. Limnol. Oceanogr. 45: 1681-1688.
22. MANZ, W., R. AMANN, W. LUDWIG, M. WAGNER, AND K.-H. SCHLEIFER. 1992. Phylogenetic oligodeoxynucleotide probes for the major subclasses of proteobacteria: Problems and solutions. Syst. Appl. Microbiol. 15: 593-600.
23. _, _, _, M. VANCANNEYT, AND K. H. SCHLEIFER. 1996. Application of a suite of 16S rRNA-specific oligonucleotide probes designed to investigate bacteria of the phylum Cytophaga-Flavobacter-Bacteroides in the natural environment. Microbiol. U.K. 142: 1097-1106.
24. METHE, B. A., AND J. P. ZEHR. 1999. Diversity of bacterial communities in Adirondack lakes: Do species assemblages reflect lake water chemistry? Hydrobiologia 401: 77-96.
25. OUVERNEY, C. C., AND J. A. FUHRMAN. 1999. Combined microautoradiography-16S rRNA probe technique for determination of radioisotope uptake by specific microbial cell types in situ. Appl. Environ. Microbiol. 65: 1746-1752.
26. PERNTHALER, A., J. PERNTHALER, H. EILERS, AND R. AMANN. 2001. Growth patterns of two marine isolates: Adaptations to substrate patchiness? Appl. Environ. Microbiol. 67: 4077-4083.
27. PERNTHALER, J., F. O. GLOCKNER, S. UNTERHOLZNER, A. ALFREIDER, R. PSENNER, AND R. AMANN. 1998. Seasonal community and population dynamics of pelagic bacteria and archaea in a high mountain lake. Appl. Environ. Microbiol. 64: 4299-4306.
28. RAMSAY, A. J. 1974. Use of autoradiography to determine proportion of bacteria metabolizing in an aquatic habitat. J. Gen. Microbiol. 80: 363-373.
29. RAMSING, N. B., H. FOSSING, T. G. FERDELMAN, F. ANDERSEN, AND B. THAMDRUP. 1996. Distribution of bacterial populations in a stratified fjord (Mariager fjord, Denmark) quantified by in situ hybridization and related to chemical gradients in the water column. Appl. Environ. Microbiol. 62: 1391-1404.
30. ROGERS, A. W. 1979. Techniques of autoradiography. Elsevier.
31. RUSS, J. C. 1999. The image processing handbook. CRC.
32. SIMON, M., AND F. AZAM. 1989. Protein-content and protein-synthesis rates of planktonic marine-bacteria. Mar. Ecol. Prog. Ser. 51: 201-213.
33. SMITH, D. C., AND F. AZAM. 1992. A simple, economical method for measuring bacterial protein synthesis in seawater using 3H-leucine. Mar. Microb. Food Webs 6: 107-114.
34. TABOR, P. S., AND R. A. NEIHOF. 1982. Improved microautoradiographic method to determine individual microorganisms active in substrate uptake in natural waters. Appl. Environ. Microbiol. 44: 945-953.
35. VILES, C. L., AND M. E. SIERACKI. 1992. Measurement of marine picoplankton cell-size by using a cooled, charge-coupled device camera with image-analyzed fluorescence microscopy. Appl. Environ. Microbiol. 58: 584-592.
36. ZARDA, B., D. HAHN, A. CHATZINOTAS, W. SCHONHUBER, A. NEEF, R. I. AMANN, AND J. ZEYER. 1997. Analysis of bacterial community structure in bulk soil by in situ hybridization. Arch. Microbiol. 168: 185-192.
37. ZUBKOV, M. V., B. M. FUCHS, S. D. ARCHER, R. P. KIENE, R. AMANN, AND P. H. BURKILL. 2001a. Linking the composition of bacterioplankton to rapid turnover of dissolved dimethylsulphoniopropionate in an algal bloom in the North Sea. Environ. Microbiol. 3: 304-311.
38. _, _, P. H. BURKILL, AND R. AMANN. 2001b. Comparison of cellular and biomass specific activities of dominant bacterioplankton groups in stratified waters of the Celtic Sea. Appl. Environ. Microbiol. 67: 5210-5218.