Low fractions of active bacteria in natural aquatic communities?

Aquatic Microbial Ecology

Volumn 31, Issue 2, 2003, Pages 203-208

  Smith, Erik M ^a   Del Giorgio, Paul A ^a  

^a UNIVERSITÉ DU QUÉBEC À MONTRÉAL   (Canada)

Author keywords

Bacteria; Cell specific activity; Methodological approaches; Physiological diversity

Indexed keywords

BACTERIA (MICROORGANISMS);

EID: 0037435039     PISSN: 09483055     EISSN: None     Source Type: Journal    
DOI: 10.3354/ame031203     Document Type: Article

References (52)

1. Almeida MA, Cunha MA, Alcantara F (2001) Physiological responses of marine and brackish water bacterial assemblages in a tidal estuary (Ria de Aveiro, Portugal). Aquat Microb Ecol 25:113-125
2. Baily CA, Neihof RA, Tabor PS (1983) Inhibitory effect of solar radiation on amino acid uptake in Chesapeake Bay bacteria. Appl Environ Microbiol 46:44-69
3. Bouvier T, del Giorgio PA (2003) Sensitivity of fluorescence in situ hybridization (FISH): patterns in cell detection in the domain Bacteria. FEMS Microbiol Ecol (in press)
4. Buck KR, Chavez FP, Campbell L (1996) Basin-wide distributions of living carbon components and the inverted trophic pyramid of the central gyre of the North Atlantic Ocean, summer 1993. Aquat Microbiol Ecol 10:283-298
5. Bunch JN, Harland RC (1990) Bacterial production in the bottom surface of sea ice in the Canadian subarctic. Can J Fish Aquat Sci 47:1986-1995
6. Cottrell MT, Kirchman DL (2000) Natural assemblage of marine proteobacteria and members of Cytopha-Flavobacter cluster consuming low- and high-molecular-weight dissolved organic matter. Appl Environ Microbiol 66:1692-1697
7. del Giorgio PA, Bouvier T (2002) Linking the physiologic and phylogenetic successions in free-living bacterial communities along an estuarine salinity gradient. Limnol Oceanogr 47:471-486
8. del Giorgio PA, Prairie YT, Bird DF (1997) Coupling between rates of bacterial production and the abundance of metabolically active bacteria in lakes, counting using CTC reduction and flow cytometry. Microb Ecol 34:144-154
9. Douglas DJ, Novitsky JA, Fournier RO (1987) Microautoradiography-based enumeration of bacteria with estimates of thymidine-specific growth rates. Mar Ecol Prog Ser 36:91-99
10. Eilers HJ, Pernthaler J, Glöckner FO, Amann R (2000) Culturability and in situ abundance of pelagic bacteria from the North Sea. Appl Environ Microbiol 66:3004-3051
11. Fuchs BM, Zubkov MV, Sahm K, Burkill PH, Amann R (2000) Culturability and in situ abundance of pelagic bacteria from the North Sea. Appl Environ Microbiol 66:3044-3051
12. Fuhrman JA, Azam F (1982) Thymidine incorporation as a measure of heterotrophic bacterioplankton production in marine surface waters: evaluation and field results. Mar Biol 66:1982
13. García-Cantizano, Calderón-Paz JI, Pedrós-Alió C (1994) Thymidine incorporation in Lake Ciso: problems in estimating bacterial secondary production across oxic-anoxic interfaces. FEMS Microbiol Ecol 14:53-64
14. Glöckner, FO, Fuchs BM, Amann R (1999) Bacterioplankton compositions of lakes and oceans: a first comparison based on fluorescence in situ hybridization. Appl Environ Microbiol 65:3721-3726
15. Grossmann S (1994) Bacterial activity in sea ice and open water of the Weddell Sea, Antarctica: a microautoradiographic study. Microb Ecol 28:1-18
16. Haglund AL, Tornblom E, Bostrom B, Tranvik L (2002) Large differences in the fraction of active bacterial in plankton, sediments, and biofilm. Microb Ecol 43:232-241
17. Hermansson M, Dahlbäck B (1983) Bacterial activity at the air/water interface. Microb Ecol 9:317-328
18. Hoppe HG (1976) Determination and properties of actively metabolizing heterotrophic bacteria in the sea investigated by means of micro-autoradiography. Mar Biol 36:291-302
19. Hoppe HG (1977) Analysis of actively metabolizing bacterial populations with the autoradiographic method. In: Rheinheimer G (ed) Microbial ecology of a brackish water environment. Springer-Verlag, Berlin, p 179-197
20. Hoppe HG, Gocke K (1993) The influence of global climate and hydrography on microbial activity in the ocean: results of a N-S Atlantic transect. Proc Int Symp Environ Microbiol, Seoul National University, p 93-110
21. Joux F, Lebaron P (2000) Use of fluorescent probes to assess physiological functions of bacteria at single-cell level. Microbes Infect 2:1523-1535
22. Jugnia LB, Richardot M, Debroas D, Sime-Ngando TS, Devaux J (2000) Variations in the number of active bacteria in the euphotic zone of a recently flooded reservoir. Aquat Microb Ecol 22:251-259
23. Karner M, Fuhrman JA (1997) Determination of active marine bacterioplankton: a comparison of universal 16S rRNA probes, autoradiography, and nucleoid staining. Appl Environ Microbiol 63:1208-1213
24. Kerkhof L, Ward BB (1993) Comparison of nucleic acid hybridization and fluorometry for measurement of the relationship between RNA/DNA ratio and growth rate in a marine bacterium. Appl Environ Microbiol 59:1303-1309
25. Marcussen B, Nielsen P, Jeppesen M (1984) Diel changes in bacterial activity determined by means of microautoradiography. Arch Hydrobiol Beih 19:141-149
26. Meyer-Reil LA (1978) Autoradiography and epifluorescence microscopy combined for the determination of number and spectrum of actively metabolizing bacteria in natural waters. Appl Environ Microbiol 36:506-512
27. Morita RY (1997) Bacteria in oligotrophic environments. Chapman & Hall, New York
28. Nedoma J, Vrba J, Hejzlar J, Šimek K, Straskrabova V (1994) N-acetylglucosamine dynamics in freshwater environments: concentration of amino sugars, extracellular enzyme activities and microbial uptake. Limnol Oceanogr 39:1088-1100
29. Novitsky JA (1983) Heterotrophic activity throughout a vertical profile of seawater and sediment in Halifax harbor, Canada. Appl Environ Microbiol 45:1753-1760
30. Oda Y, Slagman SJ, Meijer WG, Forney LJ, Gottschal JC (2000) Influence of growth rate and starvation on fluorescent in situ hybridization of Rhodopseudomonas palustris. FEMS Microbiol Ecol 32:205-213
31. Ouverney CC, Fuhrman JA (1999) Combined microautoradiography-16s rRNA probe techniques for determination of radioisotope uptake by specific microbial cell types in situ. Appl Environ Microbiol 65:1746-1752
32. Packard TT (1985) Measurements of electron transport activity in microplankton. Adv Aquat Microbiol 3:207-261
33. Pedrós-Alió C, Newell SY (1989) Microautoradiographic study of thymidine uptake in brackish waters around Sapelo Island, Georgia, USA. Mar Ecol Prog Ser 55:83-94
34. Pernthaler J, Glöckner FO, Unterholzner S, Alfreider A, Psenner R, Amann R (1998) Seasonal community and population dynamics of pelagic bacteria and archaea in a high mountain lake. Appl Environ Microbiol 64:4299-4306
35. Pernthaler A, Preston CM, Pernthaler J, Delong EF, Amann R (2002) Comparison of fluorescently labeled oligonucleotide and polynucleotide probes for the detection of pelagic marine bacteria and archaea. Appl Environ Microbiol 68:661-667
36. Riemann B, Nielson P, Jeppesen M, Marcussen B, Fuhrman JA (1984) Diel changes in bacterial biomass and growth rates in coastal environments, determined by means of thymidine incorporation into DNA, frequency of dividing cells (FDC), and microautoradiography. Mar Ecol Prog Ser 17:227-235
37. Rodriguez GG, Phipps D, Ishiguro K, Ridgway HF (1992) Use of a fluorescent redox probe for direct visualization of actively respiring bacteria. Appl Environ Microbiol 58:1801-1808
38. Ruimy R, Breittmayer V, Biovin V, Christen R (1994) Assessment of the state of cell activity of individual bacterial cells by hybridization with a ribosomal RNA-targeted fluorescently labeled oligonucleotide probe. FEMS Microbiol Ecol 15:207-213
39. Servais P, Agogue H, Courties C, Joux F, Lebaron P (2001) Are the actively respiring cells (CTC+) those responsible for bacterial production in aquatic environments? FEMS Microbiol Ecol 1215:1-9
40. Sherr BF, del Giorgio PA, Sherr EB (1999) Estimating the abundance and single-cell characteristics of respiring bacteria via the redox dye CTC. Aquat Microb Ecol 18:117-131
41. Šimek K, Budejovice C, Fuksa JK (1989) Response in bacterial activity to changing conditions in plankton - probable controlling mechanisms. Arch Hydrobiol Beih 33:239-248
42. Simon M (1985) Specific uptake rates of amino acids by attached and free-living bacteria in a mesotrophic lake. Appl Environ Microbiol 49:1254-1259
43. Simon M, Glockner FO, Amann R (1999) Different community structure and temperature optima of heterotrophic picoplankton in various regions of the southern ocean. Aquat Microb Ecol 28:275-284
44. Smith EM (1998) Coherence of microbial respiration rate and cell-specific bacterial activity in a coastal planktonic community. Aquat Microb Ecol 16:27-35
45. Stevenson LH (1978) A case for bacterial dormancy in aquatic systems. Microb Ecol 4:127-133
46. Straskrabova V, Šimek K (1984) Total and individual cell uptake of organic substances as a measure of activity of bacterioplankton. Arch Hydrobiol Beih 19:1-6
47. Tabor PS, Neihof RA (1984) Direct determination of activities for microorganisms of Chesapeake Bay populations. Appl Environ Microbiol 48:1012-1019
48. Tuomi P, Fagerbakke KM, Bratbak G, Heldal M (1995) Nutritional enrichment of a microbial community: the effects on activity, elemental composition, community structure and virus production. FEMS Microbiol Ecol 16:123-134
49. Ullrich S, Karrasch B, Hoppe HG, Jeskulke K, Mehrens M (1996) Toxic effects on bacterial metabolism of the redox dye 5-cyano-2,3-ditolyl tetrazolium chloride. Appl Environ Microbiol 62:4587-4593
50. Ullrich S, Karrasch B, Hoppe HG (1999) Is the CTC dye technique an adequate approach for estimating active bacterial cells? Aquat Microb Ecol 17:207-209
51. Williams SC, Hong Y, Danavall DCA, Howard-Jones MH, Gibson D, Frischer ME, Verity PG (1998) Distinguishing between living and nonliving bacteria: evaluation of the vital stain propidium iodide and the combined use with molecular probes in aquatic samples. J Microbiol Methods 32:225-236
52. Zubkov MV, Fuchs BM, Tarran GA, Burkill PH, Amann R (2002) Mesoscale distribution of dominant bacterioplankton groups in the northern North Sea in early summer. Aquat Microb Ecol 29:135-144