Time course of fluorescence intensity and protein expression in HeLa cells stably transfected with hrGFP

Molecules and Cells

Volumn 15, Issue 3, 2003, Pages 341-348

  Kirsch, Petra ^a,b,c   Hafner, Mathias ^d   Zentgraf, Hanswalter ^a   Schilling, Lothar ^b  

^a GERMAN CANCER RESEARCH CENTER DKFZ   (Germany)

^b UNIVERSITY OF HEIDELBERG   (Germany)

^c BOEHRINGER INGELHEIM PHARMA GMBH AND CO KG   (Germany)

^d UNIVERSITY OF APPLIED SCIENCES   (Germany)

Author keywords

HeLa cells; hrGFP; Live cell fluorescence microscopy; PCR analysis; Stable transfection; Western blot

Indexed keywords

GREEN FLUORESCENT PROTEIN; NEOMYCIN; PROPIDIUM IODIDE; RECOMBINANT PROTEIN; PHOTOPROTEIN;

AMINO ACID SEQUENCE; ARTICLE; CELL DEATH; CONTROLLED STUDY; CYTOMEGALOVIRUS; DOWN REGULATION; FLUORESCENCE MICROSCOPY; GENE CASSETTE; GENE INSERTION; GENETIC CODE; GENETIC TRANSFECTION; HELA CELL; HUMAN; HUMAN CELL; HUMAN GENOME; IMMUNOBLOTTING; INTERNAL RIBOSOME ENTRY SITE; METHODOLOGY; MOLECULAR CLONING; NONHUMAN; PLASMID; POLYMERASE CHAIN REACTION; PROMOTER REGION; PROTEIN EXPRESSION; PROTEIN PROCESSING; RENILLA; REVERSE TRANSCRIPTION; ANIMAL; ANTHOZOA; CELL SURVIVAL; COMPARATIVE STUDY; GENE EXPRESSION; GENETICS; TIME; WESTERN BLOTTING;

AEQUOREA VICTORIA; CUCUMBER MOSAIC VIRUS; CYTOMEGALOVIRUS; RENILLA; RENILLA MULLERI; RENILLA RENIFORMIS;

ANIMALS; ANTHOZOA; BLOTTING, WESTERN; CELL SURVIVAL; GENE EXPRESSION; GREEN FLUORESCENT PROTEINS; HELA CELLS; HUMANS; LUMINESCENT PROTEINS; MICROSCOPY, FLUORESCENCE; PROPIDIUM; TIME FACTORS; TRANSFECTION;

EID: 2142805257     PISSN: 10168478     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (40)

1. Alexander, L., Lee, H., Rosenzweig, M., Jung, J. U., and Desrosiers, R. C. (1997) EGFP-containing vector system that facilitates stable and transient expression assays. Biotechniques 23, 64-66.
2. Attwood, J. T., Yung, R. L., and Richardson, B. C. (2002) DNA methylation and the regulation of gene transcription. Cell. Mol. Life Sci. 59, 241-257.
3. Bestor, T. H. (2000) Gene silencing as a threat to the success of gene therapy. J. Clin. Invest. 105, 409-411.
4. Boffa, L. C., Vidali, G., Mann, R. S., and Allfrey, V. G. (1978) Suppression of histone deacetylation in vivo and in vitro by sodium butyrate. J. Biol. Chem. 253, 3364-3366.
5. Chalfie, M., Tu, Y., Euskirchen, G., Ward, W. W., and Prasher, D. C. (1994) Green fluorescent protein as a marker for gene expression. Science 263, 802-805.
6. Chen, C. and Okayama, H. (1987) High-efficiency transformation of mammalian cells by plasmid DNA. Mol. Cell. Biol. 7, 2745-2752.
7. Chishima, T., Miyagi, Y., Wang, X., Yamaoka, H., Shimada, H., Moossa, A. R., and Hoffman, R. M. (1997) Cancer invasion and micrometastasis visualized in live tissue by green fluorescent protein expression. Cancer Res. 57, 2042-2047.
8. Elliott, G. and O'Hare, P. (1999) Intercellular trafficking of VP22-GFP fusion proteins. Gene Ther. 6, 149-151.
9. Fillmore, H. L., Shurm, J., Furqueron, P., Prabhu, S. S., Gillies, G. T., and Broaddus, W. C. (1999) An in vivo rat model for visualizing glioma tumor cell invasion using stable persistent expression of the green fluorescent protein. Cancer Lett. 141, 9-19.
10. Gerdes, H. H. and Kaether, C. (1996) Green fluorescent protein: applications in cell biology. FEBS Lett. 389, 44-47.
11. Gorman, C. M., Howard, B. H., and Reeves, R. (1983) Expression of recombinant plasmids in mammalian cells is enhanced by sodium butyrate. Nucleic Acids Res. 11, 7631-7648.
12. Gubin, A. N., Reddy, B., Njoroge, J. M., and Miller, J. L. (1997) Long-term, stable expression of green fluorescent protein in mammalian cells. Biochem. Biophys. Res. Commun. 236, 347-350.
13. Gubin, A. N., Koduru, S., Njoroge, J. M., Bhatnagar, R., and Miller, J. L. (1999) Stable expression of green fluorescent protein after liposomal transfection of K562 cells without selective growth conditions. Biotechniques 27, 1162-1164, 1166-1170.
14. Gurtu, V., Yan, G., and Zhang, G. (1996) IRES bicistronic expression vectors for efficient creation of stable mammalian cell lines. Biochem. Biophys. Res. Commun. 229, 295-298.
15. Hadjantonakis, A. K. and Nagy, A. (2001) The color of mice: in the light of GFP-variant reporters. Histochem. Cell Biol. 115, 49-58.
16. Hanazono, Y., Yu, J. M., Dunbar, C. E., and Emmons, R. V. (1997) Green fluorescent protein retroviral vectors: low titer and high recombination frequency suggest a selective disadvantage. Hum. Gene Ther. 8, 1313-1319.
17. Inouye, S. and Tsuji, F. I. (1994) Aequorea green fluorescent protein. Expression of the gene and fluorescence characteristics of the recombinant protein. FEBS Lett. 341, 277-280.
18. Jung, S., Ackerley, C., Ivanchuk, S., Mondal, S., Becker, L. E., and Rutka, J. T. (2001) Tracking the invasiveness of human astrocytoma cells by using green fluorescent protein in an organotypical brain slice model. J. Neurosurg. 94, 80-89.
19. Jung, S., Kim, H. W., Lee, J. H., Kang, S. S., Rhu, H. H., Jeong, Y. I., Yang, S. Y., Chung, H. Y., Bae, C. S., Choi, C., Shin, B. A., Kim, K. K., and Ahn, K. Y. (2002) Brain tumor invasion model system using organotypic brain-slice culture as an alternative to in vivo model. J. Cancer Res. Clin. Oncol. 128, 469-476.
20. Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685.
21. Leffel, S. M., Mabon, S. A., and Stewart, C. N. (1997) Applications of green fluorescent protein in plants. Biotechniques 23, 912-918.
22. Liu, H. S., Jan, M. S., Chou, C. K., Chen, P. H., and Ke, N. J. (1999) Is green fluorescent protein toxic to the living cells? Biochem. Biophys. Res. Commun. 260, 712-717.
23. Luciani, A. M., Rosi, A., Matarrese, P., Arancia, G., Guidoni, L., and Viti, V. (2001) Changes in cell volume and internal sodium concentration in HeLa cells during exponential growth and following lonidamine treatment. Eur. J. Cell Biol. 80, 187-195.
24. Migliaccio, A. R., Bengra, C., Ling, J., Pi, W., Li, C., Zeng, S., Keskintepe, M., Whitney, B., Sanchez, M., Migliaccio, G., and Tuan, D. (2000) Stable and unstable transgene integration sites in the human genome: extinction of the Green Fluorescent Protein transgene in K562 cells. Gene 256, 197-214.
25. Mohanam, S., Jasti, S. L., Kondraganti, S. R., Chandrasekar, N., Kin, Y., Fuller, G. N., Lakka, S. S., Kyritsis, A. P., Dinh, D. H., Olivero, W. C., Gujrati, M., Yung, W. K., and Rao, J. S. (2001) Stable transfection of urokinase-type plasminogen activator antisense construct modulates invasion of human glioblastoma cells. Clin. Cancer Res. 7, 2519-2526.
26. Niswender, K. D., Blackman, S. M., Rohde, L., Magnuson, M. A., and Piston, D. W. (1995) Quantitative imaging of green fluorescent protein in cultured cells: comparison of microscopic techniques, use in fusion proteins and detection limits. J. Microsci. 180, 109-116.
27. Plasterk, R. H. and Ketting, R. F. (2000) The silence of the genes. Curr. Opin. Genet. Dev. 10, 562-567.
28. Prasher, D. C., Eckenrode, V. K., Ward, W. W., Prendergast, F. G., and Cormier, M. J. (1992) Primary structure of the Aequorea victoria green-fluorescent protein. Gene 111, 229-233.
29. Saleh, M. (1997) A retroviral vector that allows efficient co-expression of two genes and the versatility of alternate selection markers. Hum. Gene Ther. 8, 979-983.
30. Spergel, D. J., Kruth, U., Shimshek, D. R., Sprengel, R., and Seeburg, P. H. (2001) Using reporter genes to label selected neuronal populations in transgenic mice for gene promoter, anatomical, and physiological studies. Prog. Neurobiol. 63, 673-686.
31. Subramanian, S. and Srienc, F. (1996) Quantitative analysis of transient gene expression in mammalian cells using the green fluorescent protein. J. Biotechnol. 49, 137-151.
32. Sun, B., Xu, P., and Salvaterra, P. M. (1999) Dynamic visualization of nervous system in live Drosophila. Proc. Natl. Acad. Sci. USA 96, 10438-10443.
33. Tsien, R. Y. (1998) The green fluorescent protein. Annu. Rev. Biochem 67, 509-544.
34. Tucker, K. L. (2001) In vivo imaging of the mammalian nervous system using fluorescent proteins. Histochem Cell Biol. 115, 31-39.
35. Vajkoczy, P., Schilling, L., Ullrich, A., Schmiedek, P., and Menger, M. D. (1998) Characterization of angiogenesis and microcirculation of high-grade glioma: an intravital multifluorescence microscopic approach in the athymic nude mouse. J. Cereb. Blood Flow Metab. 18, 510-520.
36. Wakamatsu, Y., Pristyazhnyuk, S., Kinoshita, M., Tanaka, M., and Ozato, K. (2001) The see-through medaka: a fish model that is transparent throughout life. Proc. Natl. Acad. Sci. USA 98, 10046-10050.
37. Wassenegger, M. (2002) Gene silencing. Int. Rev. Cytol. 219, 61-113.
38. Wybranietz, W. A., Prinz, F., Spiegel, M., Schenk, A., Bitzer, M., Gregor, M., and Lauer, U. M. (1999) Quantification of VP22-GFP spread by direct fluorescence in 15 commonly used cell lines. J. Gene Med. 1, 265-274.
39. Yang, M., Baranov, E., Li, X. M., Wang, J. W., Jiang, P., Li, L., Moossa, A. R., Penman, S., and Hoffman, R. M. (2001) Whole-body and intravital optical imaging of angiogenesis in orthotopically implanted tumors. Proc. Natl. Acad Sci. USA 98, 2616-2621.
40. Zeyda, M., Borth, N., Kunert, R., and Katinger, H. (1999) Optimization of sorting conditions for the selection of stable, high-producing mammalian cell lines. Biotechnol. Prog. 15, 953-957.