Optimization of sorting conditions for the selection of stable, high-producing mammalian cell lines

Biotechnology Progress

Volumn 15, Issue 5, 1999, Pages 953-957

  Zeyda, Max ^a   Borth, Nicole ^a   Kunert, Renate ^a   Katinger, Hermann ^a  

^a UNIVERSITY OF AGRICULTURAL SCIENCES   (Austria)

Author keywords

[No Author keywords available]

Indexed keywords

CELL LINE; FIBROBLAST; GREEN FLUORESCENT PROTEIN; PLASMID; PROCESS OPTIMIZATION;

BATCH CELL CULTURE; CELLS; CLONING; GROWTH KINETICS; KINETIC THEORY; MATHEMATICAL MODELS; PRODUCTION ENGINEERING; PROTEINS;

FIBROBLAST CELLS; GREEN FLUORESCENT PROTEIN; TRANSFECTION;

BIOTECHNOLOGY;

EID: 0033198746     PISSN: 87567938     EISSN: None     Source Type: Journal    
DOI: 10.1021/bp990089g     Document Type: Article

References (15)

1. Cherlet, M.; Kromenaker, S. J.; Srienc, F. Surface IgG content of murine hybridomas: Direct evidence for variations of antibody secretion rates during the cell cycle. Biotechnol. Bioeng. 1995, 47, 535-540.
2. Gu, M. B.; Kern, J, A.; Todd, P.; Kompala, D. S. Effect of amplification of dhfr and lacZ genes on growth and β-galactosidase expression in suspension cultures of recombinant CHO cells. Cytotechnology 1992, 9, 237-245.
3. Gu, M. B.; Todd, P.; Kompala, D. S. Foreign gene expression during the cell cycle phases in recombinant CHO cells. Biotechnol. Bioeng. 1993, 42, 1113-1123.
4. Gubin, A. N.; Reddy, B.; Njoroge, J. M.; Miller, J. L. Long-term, stable expression of green fluorescent protein in mammalian cells. Biochem. Biophys. Res. Commun. 1997, 236, 347-350.
5. Jantscheff, P.; Winkler, L.; Karawajew, L.; Kaiser, G.; Böttger, V.; Micheel, B. Hybrid hybridomas producing bispecific antibodies to CEA and peroxidase isolated by a combination of HAT medium selection and fluorescence activated cell sorting. J. Immunol. Methods 1993, 163, 91-97.
6. Kim, S. J.; Kim, N. S.; Ryu, C. J.; Hong, H. J.; Lee, G. M. Characterization of chimeric antibody producing CHO cells in the course of dihydrofolate reductase-mediated gene amplification and their stability in the absence of selective pressure. Biotechnol. Bioeng. 1998, 58, 73-84.
7. Kromenaker, S. J.; Srienc, F. Stability of producer hybridoma cell lines after cell sorting: a case study. Biotechnol. Prog. 1994, 10, 299-307.
8. Lybarger, L.; Dempsey, D.; Franek, K. J.; Chervenak, R. Rapid generation and flow cytometric analysis of stable GFP-expressing cells, Cytometry 1996, 25, 211-220.
9. Marder, P.; Maciak, R. S.; Fouts, R. L.; Baker, R. S.; Starling, J. J. Selective cloning of hybridoma cells for enhanced immunoglobulin production using flow cytometric cell sorting and automated laser nephelometry. Cytometry 1990, 11, 498-505.
10. McKinney, K. L.; Dilwith, R.; Belfort, G. Manipulation of heterogeneous hybridoma cultures for overproduction of monoclonal antibodies. Biotechnol. Prog. 1991, 7, 445-454.
11. Morrison, C. J.; McMaster, W. R.; Piret, J. M. Differential stability of proteolytically active and inactive recombinant metalloproteinase in Chinese Hamster Ovary cells. Biotechnol. Bioeng. 1997, 55, 6, 594-600.
12. Rosenblatt, J. I.; Hokanson, J. A.; McLaughlin, S. R.; Leary, J. F. Theoretical basis for sampling statistics useful for detecting and isolating rare cells using flow cytometry and cell sorting. Cytometry 1997, 27, 233-238.
13. Sen, S.; Hu, W.-S.; Srienc, F. Flow Cytometric study of hybridoma cell culture: Correlation between cell surface fluorescence and IgG production rate. Enzyme Microb. Technol. 1990, 12, 571-576.
14. Subramanian, S.; Srienc, F. Quantitative analysis of transient gene expression in mammalian cells using the green fluorescent protein. J. Biotechnol. 1996, 49, 137-151.
15. Zhang, G.; Gurtu, V.; Kain, S. R. An enhanced fluorescent protein allows sensitive detection of gene transfer in mammalian cells. Biochem. Biophys. Res. Commun. 1996, 227, 707-711.