Crystal structure of the alcohol dehydrogenase from the hyperthermophilic archaeon Sulfolobus solfataricus at 1.85 Å resolution

Journal of Molecular Biology

Volumn 318, Issue 2, 2002, Pages 463-477

  Esposito, Luciana ^a   Sica, Filomena ^b   Raia, Carlo Antonio ^c   Giordano, Antonietta ^c   Rossi, Mosè ^c,d   Mazzarella, Lelio ^a,b,d   Zagari, Adriana ^a,b,d  

^a CNR   (Italy)

^b UNIVERSITY OF NAPLES FEDERICO II   (Italy)

^c INSTITUTE OF PROTEIN BIOCHEMISTRY   (Italy)

^d CEINGE BIOTECNOLOGIE AVANZATE   (Italy)

Author keywords

Alcohol dehydrogenase; Archaea; Protein structure; X ray crystallography; Zinc enzyme

Indexed keywords

ALCOHOL DEHYDROGENASE; BACTERIAL ENZYME; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE; SELENOMETHIONINE; TETRAMER; WATER; ZINC ION;

ARCHAEBACTERIUM; ARTICLE; BINDING SITE; CRYSTAL STRUCTURE; DIFFRACTION; ENZYME ACTIVE SITE; ENZYME BINDING; ENZYME STRUCTURE; ENZYME SUBSTRATE; METAL BINDING; MOLECULAR DYNAMICS; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; SEQUENCE HOMOLOGY; STRUCTURE ANALYSIS; SULFOLOBUS SOLFATARICUS; THERMOPHILIC BACTERIUM; X RAY CRYSTALLOGRAPHY;

ARCHAEA; BACTERIA (MICROORGANISMS); HOMO; SULFOLOBUS; SULFOLOBUS SOLFATARICUS;

EID: 0036301867     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-2836(02)00088-8     Document Type: Article

References (51)

1. Characteristics of short-chain alcohol dehydrogenases and related enzymes
2. Nomenclature of alcohol dehydrogenases
3. Cloning and sequencing of the gene encoding the 72-kDa dehydrogenase subunit of alcohol dehydrogenase from Acetobacter aceti
4. Three-dimensional structure of horse liver alcohol dehydrogenase at 2.4 Å resolution
5. Structure of a triclinic ternary complex of horse liver alcohol dehydrogenase at 2.9 Å resolution
6. Alcohol dehydrogenase
7. On the enzymatic activation of NADH
8. Three-dimensional structures of the three human class I alcohol dehydrogenases
9. Structure of human χχ alcohol dehydrogenase: A glutathione-dependent formaldehyde dehydrogenase
10. X-ray structure of human class IV σσ alcohol dehydrogenase. Structural basis for substrate specificity
11. Crystal structures of mouse class II alcohol dehydrogenase reveal determinants of substrate specificity and catalytic efficiency
12. Crystal structure of cod liver class I alcohol dehydrogenase: Substrate pocket and structurally variable segments
13. NADP-dependent bacterial alcohol dehydrogenases: Crystal structure, cofactor-binding and cofactor specificity of the ADHs of Clostridium beijerinckii and Thermoanaerobacter brockii
14. +-dependent alcohol dehydrogenase. Purification and properties
15. Archaea and the prokaryote-to-eukaryote transition
16. Enzymes and proteins from organisms that grow near and above 100°C
17. Thermostable NAD(+)-dependent alcohol dehydrogenase from Sulfolobus solfataricus: Gene and protein sequence determination and relationship to other alcohol dehydrogenases
18. + dependent alcohol dehydrogenase from Sulfolobus solfataricus: Structural and functional features
19. Cloning and overexpression in Escherichia coli of the genes encoding NAD-dependent alcohol dehydrogenase from two Sulfolobus species
20. Alcohol dehydrogenase from Sulfolobus solfataricus
21. Crystal structure of the NADP(H)-dependent ketose reductase from Bemisia argentifolii at 2.3 Å resolution
22. Interdomain motion in liver alcohol dehydrogenase. Structural and energetic analysis of the hinge bending mode
23. Dynamics in alcohol dehydrogenase elucidated from crystallographic investigations
24. Spectroscopic studies of inhibited alcohol dehydrogenase from Thermoanaerobacter brockii: Proposed structure for the catalytic intermediate state
25. Protein thermostability above 100°C: A key role for ionic interactions
26. The stability of salt bridges at high temperatures: Implications for hyperthermophilic proteins
27. Crystallography and NMR system: A new software suite for macromolecular structure determination
28. Progressive sequence alignment and molecular evolution of the Zn-containing alcohol dehydrogenase family
29. Carboxyl groups near the active site zinc contribute to catalysis in yeast alcohol dehydrogenase
30. Refined crystal structure of liver alcohol dehydrogenase-NADH complex at 1.8 Å resolution
31. On the role of Glu-68 in alcohol dehydrogenase
32. Aromatic clusters: A determinant of thermal stability of thermophilic proteins
33. Stereospecific oxidation of secondary alcohols by human alcohol dehydrogenases
34. Control of coenzyme binding to horse liver alcohol dehydrogenase
35. Importance of the structural zinc atom for the stability of yeast alcohol dehydrogenase
36. Zinc coordination, function, structure of zinc enzymes and other proteins
37. The crystal structure of glucose dehydrogenase from Thermoplasma acidophilum
38. Replacing the glutamate ligand in the structural zinc site of Sulfolobus solfataricus alcohol dehydrogenase with a cysteine decreases thermostability
39. Features of structural zinc in mammalian alcohol dehydrogenase. Site-directed mutagenesis of the zinc ligands
40. Asn249Tyr substitution at the coenzyme binding domain activates Sulfolobus solfataricus alcohol dehydrogenase and increases its thermal stability
41. +-dependent alcohol dehydrogenase from the extreme thermophilic archaebacterium Sulfolobus solfataricus
42. Processing of X-ray diffraction data collected in oscillation mode
43. Automated MAD and MIR structure solution
44. Density modification for macromolecular phase improvement
45. wARP: Improvement and extension of crystallographic phase by weighted averaging of multiple-refined dummy atomic models
46. Improved methods for building protein models in electron density maps and the location of errors in these models
47. PROCHECK: A program to check the stereochemical quality of protein structures
48. MOLSCRIPT: A program to produce both detailed and schematic plots of protein structures
49. Alignment of three-dimensional protein structures: Network server for database searching
50. Raster3D: Photo-realistic molecular graphics
51. An extensively modified version of MolScript that includes greatly enhanced coloring capabilities