Is the critical role of loop 3 of Escherichia coli 6-hydroxymethyl-7,8- dihydropterin pyrophosphokinase in catalysis due to loop-3 residues arginine-84 and tryptophan-89? Site-directed mutagenesis, biochemical, and crystallographic studies

Biochemistry

Volumn 44, Issue 24, 2005, Pages 8590-8599

  Li, Yue ^a   Blaszczyk, Jaroslaw ^b,c   Wu, Yan ^a   Shi, Genbin ^a   Ji, Xinhua ^b   Yan, Honggao ^a  

^a Michigan State University   (United States)

^b NATIONAL CANCER INSTITUTE   (United States)

^c MICHIGAN STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOCHEMISTRY; CATALYSIS; CRYSTAL STRUCTURE; MUTAGENESIS; RATE CONSTANTS; SUBSTRATES; X RAY CRYSTALLOGRAPHY;

ALANINE; DELETION MUTAGENESIS; DISSOCIATION CONSTANTS; KINETIC CONSTANTS;

ESCHERICHIA COLI;

6 HYDROXYMETHYL 7,8 DIHYDROPTERIN PYROPHOSPHOKINASE; ADENOSINE TRIPHOSPHATE MAGNESIUM; ARGININE; GUANIDINE; INDOLE DERIVATIVE; PHOSPHOTRANSFERASE; TRYPTOPHAN; UNCLASSIFIED DRUG;

AMINO ACID SUBSTITUTION; ARTICLE; CATALYSIS; CHEMICAL REACTION; COMPLEX FORMATION; CONFORMATIONAL TRANSITION; CRYSTAL STRUCTURE; DELETION MUTANT; DISSOCIATION CONSTANT; ESCHERICHIA COLI; KINETICS; MOLECULAR INTERACTION; NONHUMAN; PRIORITY JOURNAL; REACTION ANALYSIS; SITE DIRECTED MUTAGENESIS; X RAY CRYSTALLOGRAPHY;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; BASE SEQUENCE; DIPHOSPHOTRANSFERASES; DNA PRIMERS; ESCHERICHIA COLI; KINETICS; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; POLYMERASE CHAIN REACTION; PROTEIN STRUCTURE, SECONDARY; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT PROTEINS; THERMODYNAMICS;

ESCHERICHIA COLI;

EID: 20544448718     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi0503495     Document Type: Article

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