Catalytic roles of arginine residues 82 and 92 of Escherichia coli 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase: Site-directed mutagenesis and biochemical studies

Biochemistry

Volumn 42, Issue 6, 2003, Pages 1581-1588

  Li, Yue ^a   Wu, Yan ^a   Blaszczyk, Jaroslaw ^b   Ji, Xinhua ^b   Yan, Honggao ^a  

^a Michigan State University   (United States)

^b NATIONAL CANCER INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BINDING ENERGY; BIOCHEMISTRY; ENZYME KINETICS; ESCHERICHIA COLI; HYDROGEN BONDS; MOLECULAR STRUCTURE; MUTAGENESIS; PHOSPHATES; RATE CONSTANTS;

MUTATIONS;

CATALYST ACTIVITY;

6 HYDROXYMETHYL 7,8 DIHYRODPTERIN PYROPHOSPHOKINASE; ADENOSINE TRIPHOSPHATE; ADENOSINE TRIPHOSPHATE DERIVATIVE; ADENOSINE TRIPHOSPHATE MAGNESIUM; ALANINE; ALPHA,BETA METHYLENEADENOSINE TRIPHOSPHATE; ARGININE; GUANIDINE; PHOSPHATE; PHOSPHOTRANSFERASE; PTERIN DERIVATIVE; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ARTICLE; BINDING KINETICS; BIOCHEMISTRY; CATALYSIS; CHEMICAL STRUCTURE; DISSOCIATION CONSTANT; ENERGY; ENZYME SUBSTRATE COMPLEX; ESCHERICHIA COLI; GENETIC CONSERVATION; HYDROGEN BOND; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS; SURFACE CHARGE; THERMODYNAMICS;

AMINO ACID SUBSTITUTION; ARGININE; BINDING SITES; CATALYSIS; CATALYTIC DOMAIN; DIPHOSPHOTRANSFERASES; ESCHERICHIA COLI PROTEINS; KINETICS; MUTAGENESIS, SITE-DIRECTED; PROTEIN CONFORMATION; RECOMBINANT FUSION PROTEINS; SPECTROMETRY, FLUORESCENCE; SUBSTRATE SPECIFICITY; THERMODYNAMICS;

ESCHERICHIA COLI; NEGIBACTERIA;

EID: 0037452534     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi026800z     Document Type: Article

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