Gene regulation in an MCF-7 cell line that naturally expresses an estrogen receptor unable to directly bind DNA

Molecular and Cellular Endocrinology

Volumn 238, Issue 1-2, 2005, Pages 9-25

  Pentecost, Brian T ^a,b   Bradley, L M ^b   Gierthy, J F ^a,b   Ding, Y ^a   Fasco, M J ^a,b  

^a WADSWORTH CENTER   (United States)

^b UNIVERSITY AT ALBANY   (United States)

Author keywords

Alternate splicing; Estrogen receptor; Gene array analysis; Gene expression regulation; Real time PCR analysis; siRNA

Indexed keywords

1,4 DIAMINO 1,4 BIS(2 AMINOPHENYLTHIO) 2,3 DICYANOBUTADIENE; 4 (4 FLUOROPHENYL) 2 (4 METHYLSULFINYLPHENYL) 5 (4 PYRIDYL)IMIDAZOLE; CARBONATE DEHYDRATASE; CARBONATE DEHYDRATASE XII; DNA; EARLY GROWTH RESPONSE FACTOR 1; EARLY GROWTH RESPONSE FACTOR 3; ENZYME INHIBITOR; ESTRADIOL; ESTRADIOL RECEPTOR; FIBRINOGEN; FIBRINOGEN B BETA; INOSITOL TRISPHOSPHATE; LY 29402; MESSENGER RNA; MITOGEN ACTIVATED PROTEIN KINASE 1; MITOGEN ACTIVATED PROTEIN KINASE 3; PROGESTERONE RECEPTOR; PROTEIN MYB; PROTEIN PS2; SERINE PROTEINASE; SERINE PROTEINASE 23; SMALL INTERFERING RNA; STROMAL CELL DERIVED FACTOR 1; TAMOXIFEN; UNCLASSIFIED DRUG; ZINC FINGER PROTEIN;

ALTERNATIVE RNA SPLICING; ARTICLE; CELL STRAIN MCF 7; CONTROLLED STUDY; CYTOPLASM; DNA BINDING; DNA MICROARRAY; DOWN REGULATION; ENZYME INHIBITION; EXON; FOWL; GENE CONTROL; GENE EXPRESSION; HUMAN; HUMAN CELL; INTERMETHOD COMPARISON; MOLECULAR MODEL; MYOBLAST; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN INDUCTION; PROTEIN LOCALIZATION; PROTEIN PHOSPHORYLATION; PROTEIN PROTEIN INTERACTION; REACTION TIME; REAL TIME POLYMERASE CHAIN REACTION; SEQUENCE HOMOLOGY; SIGNAL TRANSDUCTION; UPREGULATION; VIRUS ONCOGENE; ZINC FINGER MOTIF;

BREAST NEOPLASMS; CELL LINE, TUMOR; CELL PROLIFERATION; CLONE CELLS; DOSE-RESPONSE RELATIONSHIP, DRUG; ESTRADIOL; ESTROGEN RECEPTOR ALPHA; FIBRINOGEN; GENE EXPRESSION REGULATION, NEOPLASTIC; GENE TRANSFER TECHNIQUES; HUMANS; NEOPLASMS, HORMONE-DEPENDENT; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS; POLYMERASE CHAIN REACTION; RECEPTORS, G-PROTEIN-COUPLED; RECEPTORS, PROGESTERONE; RNA INTERFERENCE; RNA, SMALL INTERFERING; TUMOR SUPPRESSOR PROTEINS;

EID: 20444392755     PISSN: 03037207     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mce.2005.04.005     Document Type: Article

References (52)

1. K.F. Arcaro, and J.F. Gierthy Assessing modulation of estrogenic activity of environmental and pharmaceutical compounds using MCF-7 focus assay Methods Mol. Biol. 176 2001 341 351
2. T. Barkhem, L.A. Haldosen, J.A. Gustafsson, and S. Nilsson pS2 Gene expression in HepG2 cells: complex regulation through crosstalk between the estrogen receptor alpha, an estrogen-responsive element, and the activator protein 1 response element Mol. Pharmacol. 61 2002 1273 1283
3. L. Bjornstrom, and M. Sjoberg Estrogen receptor-dependent activation of AP-1 via non-genomic signalling Nucl. Recept. 2 2004 3
4. C. Carmeci, D.A. Thompson, H.Z. Ring, U. Francke, and R.J. Weigel Identification of a gene (GPR30) with homology to the G-protein-coupled receptor superfamily associated with estrogen receptor expression in breast cancer Genomics 45 1997 607 617
5. K.L. Chambliss, I.S. Yuhanna, C. Mineo, P. Liu, Z. German, T.S. Sherman, M.E. Mendelsohn, R.G. Anderson, and P.W. Shaul Estrogen receptor alpha and endothelial nitric oxide synthase are organized into a functional signaling module in caveolae Circ. Res. 87 2000 E44 E52
6. Y. Ding, C.Y. Chan, and C.E. Lawrence Sfold web server for statistical folding and rational design of nucleic acids Nucleic Acids Res. 32 2004 W135 W141
7. I. Erenburg, B. Schachter, R. Lopez, and L. Ossowski Loss of an estrogen receptor isoform (ER alpha delta 3) in breast cancer and the consequences of its reexpression: interference with estrogen-stimulated properties of malignant transformation Mol. Endocrinol. 11 1997 2004 2015
8. V. Facchinetti, R. Lopa, F. Spreafico, F. Bolognese, R. Mantovani, F. Tavner, R. Watson, M. Introna, and J. Golay Isolation and characterization of the human A-myb promoter: regulation by NF-Y and Sp1 Oncogene 19 2000 3931 3940
9. E. Falkenstein, H.C. Tillmann, M. Christ, M. Feuring, and M. Wehling Multiple actions of steroid hormones - a focus on rapid, nongenomic effects Pharmacol. Rev. 52 2000 513 556
10. M.J. Fasco Quantitation of estrogen receptor mRNA and its alternatively spliced mRNAs in breast tumor cells and tissues Anal. Biochem. 245 1997 167 178
11. M.J. Fasco, G.J. Hurteau, and S.D. Spivack Gender-dependent expression of alpha and beta estrogen receptors in human nontumor and tumor lung tissue Mol. Cell Endocrinol. 188 2002 125 140
12. M.J. Fasco, A. Amin, B.T. Pentecost, Y. Yang, and J.F. Gierthy Phenotypic changes in MCF-7 cells during prolonged exposure to tamoxifen Mol. Cell Endocrinol. 206 2003 33 47
13. E.J. Filardo, J.A. Quinn, K.I. Bland, and A.R. Frackelton Jr. Estrogen-induced activation of Erk-1 and Erk-2 requires the G protein-coupled receptor homolog, GPR30, and occurs via trans-activation of the epidermal growth factor receptor through release of HB-EGF Mol. Endocrinol. 14 2000 1649 1660
14. J.F. Gierthy, D.W. Lincoln, K.E. Roth, S.S. Bowser, J.A. Bennett, L. Bradley, and H.W. Dickerman Estrogen-stimulation of postconfluent cell accumulation and foci formation of human MCF-7 breast cancer cells J. Cell. Biochem. 45 1991 177 187
15. A.J. Gray, J.E. Bishop, J.T. Reeves, and G.J. Laurent A alpha and B beta chains of fibrinogen stimulate proliferation of human fibroblasts J. Cell Sci. 104 Pt 2 1993 409 413
16. Q. Gu, K.S. Korach, and R.L. Moss Rapid action of 17beta-estradiol on kainate-induced currents in hippocampal neurons lacking intracellular estrogen receptors Endocrinology 140 1999 660 666
17. J.M. Hall, and K.S. Korach Stromal cell-derived factor 1, a novel target of estrogen receptor action, mediates the mitogenic effects of estradiol in ovarian and breast cancer cells Mol. Endocrinol. 17 2003 792 803
18. H. Inadera Estrogen-induced genes, WISP-2 and pS2, respond divergently to protein kinase pathway Biochem. Biophys. Res. Commun. 309 2003 272 278
19. A. Inoue, Y. Omoto, Y. Yamaguchi, R. Kiyama, and S.I. Hayashi Transcription factor EGR3 is involved in the estrogen-signaling pathway in breast cancer cells J. Mol. Endocrinol. 32 2004 649 661
20. A. Inoue, N. Yoshida, Y. Omoto, S. Oguchi, T. Yamori, R. Kiyama, and S. Hayashi Development of cDNA microarray for expression profiling of estrogen-responsive genes J. Mol. Endocrinol. 29 2002 175 192
21. M. Jakacka, M. Ito, J. Weiss, P.Y. Chien, B.D. Gehm, and J.L. Jameson Estrogen receptor binding to DNA is not required for its activity through the nonclassical AP1 pathway J. Biol. Chem. 276 2001 13615 13621
22. S.B. Jakowlew, R. Breathnach, J.M. Jeltsch, P. Masiakowski, and P. Chambon Sequence of the pS2 mRNA induced by estrogen in the human breast cancer cell line MCF-7 Nucleic Acids Res. 12 1984 2861 2878
23. M.H. Jeng, M.A. Shupnik, T.P. Bender, E.H. Westin, D. Bandyopadhyay, R. Kumar, S. Masamura, and R.J. Santen Estrogen receptor expression and function in long-term estrogen-deprived human breast cancer cells Endocrinology 139 1998 4164 4174
24. S.Y. Jiang, and V.C. Jordan Growth regulation of estrogen receptor-negative breast cancer cells transfected with complementary DNAs for estrogen receptor J. Natl. Cancer Inst. 84 1992 580 591
25. D.A. Lannigan Estrogen receptor phosphorylation Steroids 68 2003 1 9
26. B.C. Lee, T.H. Lee, S. Avraham, and H.K. Avraham Involvement of the chemokine receptor CXCR4 and its ligand stromal cell-derived factor 1alpha in breast cancer cell migration through human brain microvascular endothelial cells Mol. Cancer Res. 2 2004 327 338
27. D. Liu, H. Jia, D.I. Holmes, A. Stannard, and I. Zachary Vascular endothelial growth factor-regulated gene expression in endothelial cells: KDR-mediated induction of Egr3 and the related nuclear receptors Nur77, Nurr1, and Nor1 Arterioscler. Thromb. Vasc. Biol. 23 2003 2002 2007
28. P. Masiakowski, R. Breathnach, J. Bloch, F. Gannon, A. Krust, and P. Chambon Cloning of cDNA sequences of hormone-regulated genes from the MCF-7 human breast cancer cell line Nucleic Acids Res. 10 1982 7895 7903
29. R. Metivier, G. Penot, R.P. Carmouche, M.R. Hubner, G. Reid, S. Denger, D. Manu, H. Brand, M. Kos, V. Benes, and F. Gannon Transcriptional complexes engaged by apo-estrogen receptor-alpha isoforms have divergent outcomes EMBO J. 23 2004 3653 3666
30. J. Milanini-Mongiat, J. Pouyssegur, and G. Pages Identification of two Sp1 phosphorylation sites for p42/p44 mitogen-activated protein kinases: their implication in vascular endothelial growth factor gene transcription J. Biol. Chem. 277 2002 20631 20639
31. A. Muller, B. Homey, H. Soto, N. Ge, D. Catron, M.E. Buchanan, T. McClanahan, E. Murphy, W. Yuan, S.N. Wagner, J.L. Barrera, A. Mohar, E. Verastegui, and A. Zlotnik Involvement of chemokine receptors in breast cancer metastasis Nature 410 2001 50 56
32. L.C. Murphy, E. Leygue, H. Dotzlaw, D. Douglas, A. Coutts, and P.H. Watson Oestrogen receptor variants and mutations in human breast cancer Ann. Med. 29 1997 221 234
33. A. Nadal, M. Diaz, and M.A. Valverde The estrogen trinity: membrane, cytosolic, and nuclear effects News Physiol. Sci. 16 2001 251 255
34. S.A. Ness Myb protein specificity: evidence of a context-specific transcription factor code Blood Cells Mol. Dis. 31 2003 192 200
35. S. Nilsson, S. Makela, E. Treuter, M. Tujague, J. Thomsen, G. Andersson, E. Enmark, K. Pettersson, M. Warner, and J.A. Gustafsson Mechanisms of estrogen action Physiol. Rev. 81 2001 1535 1565
36. S. Oesterreich, P. Zhang, R.L. Guler, X. Sun, E.M. Curran, W.V. Welshons, C.K. Osborne, and A.V. Lee Re-expression of estrogen receptor alpha in estrogen receptor alpha-negative MCF-7 cells restores both estrogen and insulin-like growth factor-mediated signaling and growth Cancer Res. 61 2001 5771 5777
37. C.K. Osborne Steroid hormone receptors in breast cancer management Breast Cancer Res. Treat. 51 1998 227 238
38. B.T. Pentecost Expression and estrogen regulation of the HEM45 MRNA in human tumor lines and in the rat uterus J. Steroid Biochem. Mol. Biol. 64 1998 25 33
39. W. Porter, B. Saville, D. Hoivik, and S. Safe Functional synergy between the transcription factor Sp1 and the estrogen receptor Mol. Endocrinol. 11 1997 1569 1580
40. M. Razandi, A. Pedram, G.L. Greene, and E.R. Levin Cell membrane and nuclear estrogen receptors (ERs) originate from a single transcript: studies of ERalpha and ERbeta expressed in Chinese hamster ovary cells Mol. Endocrinol. 13 1999 307 319
41. S. Rozen, and H. Skaletsky Primer3 on the WWW for general users and for biologist programmers Methods Mol. Biol. 132 2000 365 386
42. B.J. Rybarczyk, and P.J. Simpson-Haidaris Fibrinogen assembly, secretion, and deposition into extracellular matrix by MCF-7 human breast carcinoma cells Cancer Res. 60 2000 2033 2039
43. S. Safe Transcriptional activation of genes by 17 beta-estradiol through estrogen receptor-Sp1 interactions Vitam. Horm. 62 2001 231 252
44. D.A. Simpson, S. Feeney, C. Boyle, and A.W. Stitt Retinal VEGF mRNA measured by SYBR green I fluorescence: a versatile approach to quantitative PCR Mol. Vis. 6 2000 178 183
45. P.J. Simpson-Haidaris, and B. Rybarczyk Tumors and fibrinogen. The role of fibrinogen as an extracellular matrix protein Ann. N. Y. Acad. Sci. 936 2001 406 425
46. A. Tamrazi, K.E. Carlson, J.R. Daniels, K.M. Hurth, and J.A. Katzenellenbogen Estrogen receptor dimerization: ligand binding regulates dimer affinity and dimer dissociation rate Mol. Endocrinol. 16 2002 2706 2719
47. P. Thomas, Y. Pang, E.J. Filardo, and J. Dong Identity of an estrogen membrane receptor coupled to a G-protein in human breast cancer cells Endocrinology 146 2005 624 632
48. Y. Wang, and R.J. Miksicek Identification of a dominant negative form of the human estrogen receptor Mol. Endocrinol. 5 1991 1707 1715
49. C.S. Watson, C.H. Campbell, and B. Gametchu The dynamic and elusive membrane estrogen receptor-alpha Steroids 67 2002 429 437
50. P. Webb, G.N. Lopez, R.M. Uht, and P.J. Kushner Tamoxifen activation of the estrogen receptor/AP-1 pathway: potential origin for the cell-specific estrogen-like effects of antiestrogens Mol. Endocrinol. 9 1995 443 456
51. M.X. Wu, Z. Ao, K.V. Prasad, R. Wu, and S.F. Schlossman IEX-1L, an apoptosis inhibitor involved in NF-kappaB-mediated cell survival Science 281 1998 998 1001
52. D.A. Zajchowski, R. Sager, and L. Webster Estrogen inhibits the growth of estrogen receptor-negative, but not estrogen receptor-positive, human mammary epithelial cells expressing a recombinant estrogen receptor Cancer Res. 53 1993 5004 5011