IEX-1L, an apoptosis inhibitor involved in NF-κB-mediated cell survival

Science

Volumn 281, Issue 5379, 1998, Pages 998-1001

  Wu, Mei X ^a   Ao, Zhaohui ^a   Prasad, K V S ^a   Wu, Ruilian ^a   Schlossman, Stuart F ^a  

^a HARVARD MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

NUCLEAR FACTOR;

APOPTOSIS; ARTICLE; CELL SURVIVAL; GENE CONTROL; HUMAN; HUMAN CELL; PRIORITY JOURNAL; TRANSCRIPTION REGULATION;

ANIMALS; ANTIGENS, CD95; APOPTOSIS; APOPTOSIS REGULATORY PROTEINS; CELL LINE; CELL SURVIVAL; CLONING, MOLECULAR; DNA, ANTISENSE; GENE EXPRESSION REGULATION; GENETIC VECTORS; HUMANS; IMMEDIATE-EARLY PROTEINS; JURKAT CELLS; MEMBRANE GLYCOPROTEINS; MEMBRANE PROTEINS; MICE; NEOPLASM PROTEINS; NF-KAPPA B; TRANSFECTION; TUMOR NECROSIS FACTOR-ALPHA;

EID: 0032516651     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (28)

1. R. Beyaert and W. Fiers, FEBS Lett. 340, 9 (1994); D. Wallach, Trends Biochem. Sci. 22, 107 (1997).
2. R. Beyaert and W. Fiers, FEBS Lett. 340, 9 (1994); D. Wallach, Trends Biochem. Sci. 22, 107 (1997).
3. H. Hsu, H. Shu, M. Pan, D. V. Goeddel, Cell 84, 299 (1996); Z. Liu, H. Hsu, D. V. Goeddel, M. Karin, ibid. 87, 565 (1996).
4. H. Hsu, H. Shu, M. Pan, D. V. Goeddel, Cell 84, 299 (1996); Z. Liu, H. Hsu, D. V. Goeddel, M. Karin, ibid. 87, 565 (1996).
5. P. A. Baeuerle and D. Baltimore, ibid. 87, 13 (1996); Wu et al., EMBO J. 15, 4682 (1996); S. Nagata, Cell 88, 355 (1997).
6. P. A. Baeuerle and D. Baltimore, ibid. 87, 13 (1996); Wu et al., EMBO J. 15, 4682 (1996); S. Nagata, Cell 88, 355 (1997).
7. P. A. Baeuerle and D. Baltimore, ibid. 87, 13 (1996); Wu et al., EMBO J. 15, 4682 (1996); S. Nagata, Cell 88, 355 (1997).
8. A. A. Beg and D. Baltimore, Science 274, 782 (1996); C.-Y. Wang, M. W. Mayo, A. S. Baldwin Jr., ibid., p. 784; D. J. Van Antwerp, S. J. Martin, T. Kafri, D. R. Green, I. M. Verma, ibid., p. 787.
9. A. A. Beg and D. Baltimore, Science 274, 782 (1996); C.-Y. Wang, M. W. Mayo, A. S. Baldwin Jr., ibid., p. 784; D. J. Van Antwerp, S. J. Martin, T. Kafri, D. R. Green, I. M. Verma, ibid., p. 787.
10. A. A. Beg and D. Baltimore, Science 274, 782 (1996); C.-Y. Wang, M. W. Mayo, A. S. Baldwin Jr., ibid., p. 784; D. J. Van Antwerp, S. J. Martin, T. Kafri, D. R. Green, I. M. Verma, ibid., p. 787.
11. P. Liang and A. B. Pardee, ibid. 257, 967 (1992).
12. A. D. Kondratyev, K. N. Chung, M. O. Jung, Cancer Res. 56, 1498 (1996); C. H. Charles, J. K. Yoon, J. S. Simske, L. F. Lau, Oncogene 8, 797 (1993); H. Schäfer, A. Trauzold, E. G. Siegel, U. R. Fölsch, W. E. Schmidt, Cancer Res. 56, 2641 (1996).
13. A. D. Kondratyev, K. N. Chung, M. O. Jung, Cancer Res. 56, 1498 (1996); C. H. Charles, J. K. Yoon, J. S. Simske, L. F. Lau, Oncogene 8, 797 (1993); H. Schäfer, A. Trauzold, E. G. Siegel, U. R. Fölsch, W. E. Schmidt, Cancer Res. 56, 2641 (1996).
14. A. D. Kondratyev, K. N. Chung, M. O. Jung, Cancer Res. 56, 1498 (1996); C. H. Charles, J. K. Yoon, J. S. Simske, L. F. Lau, Oncogene 8, 797 (1993); H. Schäfer, A. Trauzold, E. G. Siegel, U. R. Fölsch, W. E. Schmidt, Cancer Res. 56, 2641 (1996).
15. GenBank accession number of IEX-1L cDNA, AF039067.
16. M. Wu, R. Wu, Z. Ao, S. F. Schlossman, unpublished data. GenBank accession number of IEX-1 genomic DNA, AF071596.
17. 2-terminus of GFP using the site Eco RI-Bam HI in a pEGFP-N1 plasmid (Clontech). The resulting pEGFP-IEX-1L and pEGFP-IEX-1S were digested and ligated to the site Hind III-Bam HI in a pcDNA plasmid (Invitrogen) containing HA epitope sequence at the COOH-terminus of expressing protein. IEX-1L excised from pBluescript II/KS(-)-IEX-1L plasmid was subcloned into the Sal I-Not I site in a hygromycin-resistant gene-containing plasmid BCMGS/Hyg. pRc-Fas plasmid was a kind gift from S. M. Lehar (ImmunoGen Inc. Cambridge, MA).
18. The Ab was produced in rabbit by injecting affinity-purified glutathione S-transferase - IEX-1L fusion protein and was purified on a protein A column followed by an IEX-1L protein column. It reacted with both IEX-1L and IEX-1S proteins.
19. S. Takayama et al., Cell 80, 279 (1995); T. Torigoe et al., Cancer Res. 54, 4851 (1994); E. Yang and S. J. Korsmeyer, Blood 88, 386 (1996).
20. S. Takayama et al., Cell 80, 279 (1995); T. Torigoe et al., Cancer Res. 54, 4851 (1994); E. Yang and S. J. Korsmeyer, Blood 88, 386 (1996).
21. S. Takayama et al., Cell 80, 279 (1995); T. Torigoe et al., Cancer Res. 54, 4851 (1994); E. Yang and S. J. Korsmeyer, Blood 88, 386 (1996).
22. 6 individual clones.
23. K. Brown, S. Gerstberger, L. Carlson, G. Franzoso, U. Siebenlist, Science 267, 1485 (1995); J. A. S. Baldwin Jr., Annu. Rev. Immunol. 4, 649 (1996).
24. K. Brown, S. Gerstberger, L. Carlson, G. Franzoso, U. Siebenlist, Science 267, 1485 (1995); J. A. S. Baldwin Jr., Annu. Rev. Immunol. 4, 649 (1996).
25. A. A. Beg, W. C. Sha, R. T. Bronson, S. Ghosh, D. Baltimore, Nature 376, 167 (1995).
26. F. Kontgen et al., Genes Dev. 9, 1965 (1995).
27. K. V. S. Prasad et al., Proc. Natl. Acad. Sci. U.S.A. 94, 6346 (1997).
28. We thank I. V. Verma for Jurkat-IκBαM, D. Baltimore for p65KO3T3 cells, J. C. Reed for Bcl-2-transfected Jurkat cells, P. Anderson and H. Saito for their critical reading of the manuscript, and H. Levine for flow cytometric analysis. Supported by National Institutes of Health grants p30AI28691 (to M.X.W) and AI12069 (to S.F.S).