Cytochalasin D can improve heterologous protein productivity in adherent Chinese hamster ovary cells

Biotechnology and Bioengineering

Volumn 90, Issue 3, 2005, Pages 354-364

  Hayduk, Eric J ^a   Lee, Kelvin H ^a  

^a Cornell University   (United States)

Author keywords

Chinese hamster ovary cells; Cytochalasin D; Gene amplification; Productivity; Proteomics

Indexed keywords

ALKALINITY; CELLS; CLONING; CYTOLOGY; ELECTROPHORESIS; GENES; MASS SPECTROMETRY;

CHAPERONES; CYTOCHALASIN D; OVARY CELLS; SECRETED ALKALINE PHOSPHATASE (SEAP);

PROTEINS;

ACTIN; ACTIN CAPPING PROTEIN; ALKALINE PHOSPHATASE; CHAPERONE; COFILIN; CYCLOPHILIN; CYTOCHALASIN D; CYTOSKELETON PROTEIN; DIHYDROLIPOAMIDE SUCCINYLTRANSFERASE; ELONGATION FACTOR 1; ELONGATION FACTOR 2; EZRIN; FOLATE RECEPTOR; FUMARATE HYDRATASE; LAMIN A; METHOTREXATE; NUCLEAR PROTEIN; PROTEIN; REGULATOR PROTEIN; UNCLASSIFIED DRUG; WNT1 PROTEIN;

ANIMAL CELL; ARTICLE; CELL CYCLE; CHO CELL; CITRIC ACID CYCLE; CONTROLLED STUDY; CYTOSKELETON; FOLATE METABOLISM; GENE AMPLIFICATION; HETEROLOGOUS EXPRESSION; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; NONHUMAN; PRODUCTIVITY; PROTEIN DEGRADATION; PROTEIN ELECTROPHORESIS; PROTEIN FUNCTION; PROTEIN SYNTHESIS; PROTEOMICS; REAL TIME POLYMERASE CHAIN REACTION; RNA PROCESSING; SIGNAL TRANSDUCTION;

ANIMALS; CELL ADHESION; CELL CULTURE TECHNIQUES; CHO CELLS; CLONING, MOLECULAR; CRICETINAE; CRICETULUS; CYTOCHALASIN D; DOSE-RESPONSE RELATIONSHIP, DRUG; GENE EXPRESSION REGULATION; GENETIC ENHANCEMENT; PROTEIN ENGINEERING; RECOMBINANT PROTEINS;

CRICETINAE; CRICETULUS GRISEUS;

EID: 20344403310     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.20438     Document Type: Article

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