Comparison of automated in-gel digest methods for femtomole level samples

Electrophoresis

Volumn 24, Issue 19-20, 2003, Pages 3508-3516

  Finehout, Erin J ^a   Lee, Kelvin H ^a  

^a Cornell University ^*  (United States)

Author keywords

In gel digestion; Proteomics

Indexed keywords

GELS; MASS SPECTROMETRY; MOLECULAR BIOLOGY; PEPTIDES; RUBY;

DIGESTION METHOD; IN-GEL DIGESTS; INGEL DIGESTION; MASS SPECTROMETRY ANALYSIS; MATRIX-ASSISTED LASER DESORPTION IONIZATION MASS SPECTROMETRY; PEAK INTENSITY; PEPTIDE SEQUENCES; PROTEIN LEVEL; PROTEOMICS; SEQUENCE COVERAGES;

AUTOMATION;

ACETONITRILE; AMINO ACID; ANAZOLENE SODIUM; BETA LACTOGLOBULIN; BOVINE SERUM ALBUMIN; DYE; MYOGLOBIN; SYPRO RUBY; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ANALYTIC METHOD; ARTICLE; AUTOMATION; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; EXTRACTION; INTERMETHOD COMPARISON; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MOLECULAR WEIGHT; PROTEIN DEGRADATION; SAMPLING; SEQUENCE ANALYSIS; STAINING; STANDARD; STATISTICAL SIGNIFICANCE; TIME; TWO DIMENSIONAL GEL ELECTROPHORESIS;

EID: 0347764580     PISSN: 01730835     EISSN: None     Source Type: Journal    
DOI: 10.1002/elps.200305615     Document Type: Article

References (21)

1. Lahm, H., Langen, H., Electrophoresis 2000, 21, 2105-2114.
2. Pappin, D. J. C., Hojrup, P., Bleasby, A. J., Curr. Biol. 1993, 3, 327-332.
3. Quadroni, M., James, P., Electrophoresis 1999, 20, 664-677.
4. Courchesne, P. L., Luethy, R., Patterson, S. D., Electrophoresis 1997, 18, 369-381.
5. Rosenfeld, J., Capdevielle, J., Guillemot, J. C., Ferrara, P., Anal. Biochem. 1992, 203, 173-179.
6. Soskic, V., Godovac-Zimmermann, J., Proteomics 2001, 1, 1364-1367.
7. Borchers, C., Peter, J. F., Hall, M. C., Kunkel, T. A., Tomer, K. B., Anal. Chem. 2000, 72, 1163-1168.
8. Hellman, U., Wernstedt, C., Góñez, J., Heldin, C., Anal. Biochem. 1995, 224, 451-455.
9. http://www.abrf.org/ResearchGroups/ProteinIdentification/Eposters/ pirgprotocol.html, last accessed 16 Dec 2002
10. Wilm, M., Shevchenko, A., Houthaeve, T., Breit, S., Schweigerer, L., Fotsis, T., Mann, M., Nature 1996, 379, 466-469.
11. Katayama, H., Nagasu, T., Oda, Y., Rapid Commun. Mass Spectrom. 2001, 15, 1416-1421.
12. Speicher, K. D., Kolbas, O., Harper, S., Speicher, D., J. Biomol. Techniques 2000, 11, http://www.abrf.org/JBT/2000/june00/jun00index.html, last accessed 16 Dec 2002.
13. Shevchenko, A., Shevchenko, A., Anal. Biochem. 2001, 296, 279-283.
14. Havlis, J., Thomas, H., Sebela, M., Shevchenko A., Anal. Chem. 2003, 75, 1300-1306.
15. Lauber, W. M., Carroll, J. A., Dufield, D. R., Kiesel, J. R., Radabaugh, M. R., Malone, J. P., Electrophoresis 2001, 22, 906-918.
16. Medzihradszky, K. F., Campbell, J. M., Baldwin, M. A., Falick, A. M., Juhasz, P., Vestal, M. L., Burlingame, A. L., Anal. Chem. 2000, 72, 552-558.
17. http://www.matrixscience.com, accessed 16 Dec 2002.
18. http://www.ncbi.nlm.nih.gov/, downloaded 7-11-2002.
19. http://us.expasy.org/tools/peptide-mass.html, accessed 16 Dec 2002.
20. Bagshaw, R. D., Callahan, J. W., Mahuran, D. J., Anal. Biochem. 2000, 284, 432-435.
21. Ward, L. D., Hong, J., Simpson, R. J., in: Villafranca, J. J. (Ed.), Techniques in Protein Chemistry II, Academic Press, San Diego, CA 1991, pp. 21-30.