Genotypic and phenotypic characteristics of Pseudomonas aeruginosa isolates associated with ulcerative keratitis

Journal of Medical Microbiology

Volumn 54, Issue 6, 2005, Pages 519-526

  Winstanley, Craig ^a   Kaye, Stephen B ^a   Neal, Timothy J ^a   Chilton, Helen J ^a   Miksch, Silvia ^a   Hart, C Anthony ^a  

^a UNIVERSITY OF LIVERPOOL   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

CELL RECEPTOR; PYOVERDINE; PYOVERDINE RECEPTOR; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL GENE; BACTERIAL VIRULENCE; BACTERIUM ISOLATE; CELL MOTILITY; CORNEA ULCER; EXOS GENE; EXOU GENE; FLIC GENE; GENOTYPE; GENOTYPE PHENOTYPE CORRELATION; NONHUMAN; PHENOTYPE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PSEUDOMONAS AERUGINOSA; RANDOM AMPLIFIED POLYMORPHIC DNA; SEROTYPE; UNITED KINGDOM;

ADP RIBOSE TRANSFERASES; BACTERIAL PROTEINS; BACTERIAL TOXINS; CORNEAL ULCER; ENGLAND; GENES, BACTERIAL; GENOTYPE; HUMANS; PHENOTYPE; PSEUDOMONAS AERUGINOSA; PSEUDOMONAS INFECTIONS; SEROTYPING; VIRULENCE FACTORS;

EID: 20344380671     PISSN: 00222615     EISSN: None     Source Type: Journal    
DOI: 10.1099/jmm.0.46005-0     Document Type: Article

References (52)

1. Ahn, K. S., Ha, U., Jia, J., Wu, D. & Jin, S. (2004). The truA gene of Pseudomonas aeruginosa is required for the expression of type III secretory genes. Microbiology 150, 539-547.
2. Ajayi, T., Allmond, L. R., Sawa, T. & Wiener-Kronish, J. P. (2003). Single-nucleotide-polymorphism mapping of the Pseudomonas aeruginosa type III secretion toxins for development of a diagnostic multiplex PCR system. J Clin Microbiol 41, 3526-3531.
3. Arora, S. K., Bangera, M., Lory, S. & Ramphal, R. (2001). A genomic island in Pseudomonas aeruginosa carries the determinants of flagellin glycosylation. Proc Natl Acad Sci U S A 98, 9342-9347.
4. Beare, P. A., For, R. J., Martin, L. W. & Lamont, I. L. (2003). Siderophore-mediated cell signalling in Pseudomonas aeruginosa: divergent pathways regulate virulence factor production and siderophore receptor synthesis. Mol Microbiol 47, 195-207.
5. Berthelot, P., Attree, I., Plesiat, P., Chabert, J., de Bentzmann, S., Pozzetto, B. & Grattard, F. (2003). Genotypic and phenotypic analysis of type III secretion system in a cohort of Pseudomonas aeruginosa bacteremia isolates: evidence for a possible association between O serotypes and exo genes. J Infect Dis 188, 512-518.
6. Comolli, J. C., Hauser, A. R., Waite, L., Whitchurch, C. B., Mattick, J. S. & Engel, J. N. (1999). Pseudomonas aeruginosa gene products PilT and PilU are required for cytotoxicity in vitro and virulence in a mouse model of acute pneumonia. Infect Immun 67, 3625-3630.
7. Cowell, B. A., Weissman, B. A., Yeung, K. K., Johnson, L., Ho, S., Van, R., Bruckner, D., Mondino, B. & Fleiszig, S. M. (2003). Phenotype of Pseudomonas aeruginosa isolates causing corneal infection between 1997 and 2000. Cornea 22, 131-134.
8. Dacheux, D., Toussaint, B., Richard, M., Brochier, G., Croize, J. & Attree, I. (2000). Pseudomonas aeruginosa cystic fibrosis isolates induce rapid, type III secretion-dependent, but ExoU-independent, oncosis of macrophages and polymorphonuclear neutrophils. Infect Immun 68, 2916-2924.
9. De Chial, M., Ghysels, B., Beatson, S. A. & 9 other authors (2003). Identification of type II and type III pyoverdine receptors from Pseudomonas aeruginosa. Microbiology 149, 821-831.
10. De Vos, D., Lim, A. Jr, Pirnay, J. P., Struelens, M., Vandenvelde, C., Duinslaeger, L., Vanderkelen, A. & Cornelis, P. (1997). Direct detection and identification of Pseudomonas aeruginosa in clinical samples such as skin biopsy specimens and expectorations by multiplex PCR based on two outer membrane lipoprotein genes, oprI and oprL. J Clin Microbiol 35, 1295-1299.
11. De Vos, D., de Chial, M., Cochez, C., Jansen, S., Tümmler, B., Meyer, J. M. & Cornelis, P. (2001). Study of pyoverdine type and production by Pseudomonas aeruginosa isolated from cystic fibrosis patients: prevalence of type II pyoverdine isolates and accumulation of pyoverdine-negative mutations. Arch Microbiol 175, 384-388.
12. Ernst, R. K., D'Argenio, D. A., Ichikawa, J. K. & 12 other authors (2003). Genome mosaicism is conserved but not unique in Pseudomonas aeruginosa isolates from the airways of young children with cystic fibrosis. Environ Microbiol 5, 1341-1349.
13. Faure, K., Shimabukuro, D., Ajayi, T., Allmond, L. R., Sawa, T. & Wiener-Kronish, J. P. (2003). O-antigen serotypes and type III secretory toxins in clinical isolates of Pseudomonas aeruginosa. J Clin Microbiol 41, 2158-2160.
14. Feldman, M., Bryan, R., Rajan, S., Scheffler, L., Brunnert, S., Tang, H. & Prince, A. (1998). Role of flagella in pathogenesis of Pseudomonas aeruginosa pulmonary infection. Infect Immun 66, 43-51.
15. Feltman, H., Schulert, G., Khan, S., Jain, M., Peterson, L. & Hauser, A. R. (2001). Prevalence of type III secretion genes in clinical and environmental isolates of Pseudomonas aeruginosa. Microbiology 147, 2659-2669.
16. Finck-Barbancon, V., Goranson, J., Zhu, L., Sawa, T., Wiener-Kronish, J. P., Fleiszig, S. M., Wu, C., Mende-Mueller, L. & Frank, D. W. (1997). ExoU expression by Pseudomonas aeruginosa correlates with acute cytotoxicity and epithelial injury. Mol Microbiol 25, 547-557.
17. Fleiszig, S. M. & Evans, D. J. (2002). The pathogenesis of bacterial keratitis: studies with Pseudomonas aeruginosa. Clin Exp Optom 85, 271-278.
18. Fleiszig, S. M., Wiener-Kronish, J. P., Miyazaki, H., Vallas, V., Mostov, K. E., Kanada, D., Sawa, T., Yen, T. S. & Frank, D. W. (1997). Pseudomonas aeruginosa-mediated cytotoxicity and invasion correlate with distinct genotypes at the loci encoding exoenzyme S. Infect Immun 65, 579-586.
19. Fleiszig, S. M., Arora, S. K., Van, R. & Ramphal, R. (2001). FlhA, a component of the flagellum assembly apparatus of Pseudomonas aeruginosa, plays a role in internalization by corneal epithelial cells. Infect Immun 69, 4931-4937.
20. Fonseca, A. P., Extremina, C., Fonseca, A. F. & Sousa, J. C. (2004). Effect of subinhibitory concentration of piperacillin/tazobactam on Pseudomonas aeruginosa. J Med Microbiol 53, 903-910.
21. Hahn, H. P. (1997). The type-4 pilus is the major virulence-associated adhesin of Pseudomonas aeruginosa - a review. Gene 192, 99-108.
22. Head, N. E. & Yu, H. (2004). Cross-sectional analysis of clinical and environmental isolates of Pseudomonas aeruginosa: biofilm formation, virulence, and genome diversity. Infect Immun 72, 133-144.
23. Hirakata, Y., Finlay, B. B., Simpson, D. A., Kohno, S., Kamihira, S. & Speert, D. P. (2000). Penetration of clinical isolates of Pseudomonas aeruginosa through MDCK epithelial cell monolayers. J Infect Dis 181, 765-769.
24. Kawaharajo, K., Abe, C., Homma, J. Y., Kawano, M. & Goto, E. (1974). Corneal ulcers caused by protease and elastase from Pseudomonas aeruginosa. Jpn J Exp Med 44, 435-442.
25. Kernacki, K. A., Goebel, D. J., Poosch, M. S. & Hazlett, L. D. (1998). Early cytokine and chemokine gene expression during Pseudomonas aeruginosa corneal infection in mice. Infect Immun 66, 376-379.
26. Kessler, E., Kennah, H. E. & Brown, S. I. (1977). Pseudomonas protease: purification, partial characterization, and its effect on collagen, proteoglycan, and rabbit corneas. Invest Ophthalmol Vis Sci 16, 488-497.
27. Kus, J. V., Tullis, E., Cvitkovitch, D. G. & Burrows, L. L. (2004). Significant differences in type IV pilin allele distribution among Pseudomonas aeruginosa isolates from cystic fibrosis (CF) versus non-CF patients. Microbiology 150, 1315-1326.
28. Lanotte, P., Watt, S., Mereghetti, L., Dartiguelongue, N., Rastegar-Lari, A., Goudeau, A. & Quentin, R. (2004). Genetic features of Pseudomonas aeruginosa isolates from cystic fibrosis patients compared with those of isolates from other origins. J Med Microbiol 53, 73-81.
29. Larbig, K. D., Christmann, A., Johann, A., Klockgether, J., Hartsch, T., Merkl, R., Wiehlmann, L., Fritz, H. J. & Tümmler, B. (2002). Gene islands integrated into tRNA(Gly) genes confer genome diversity on a Pseudomonas aeruginosa clone. J Bacteriol 184, 6665-6680.
30. Lee, E. J., Cowell, B. A., Evans, D. J. & Fleiszig, S. M. (2003). Contribution of ExsA-regulated factors to corneal infection by cytotoxic and invasive Pseudomonas aeruginosa in a murine scarification model. Invest Ophthalmol Vis Sci 44, 3892-3898.
31. Lomholt, J. A., Poulsen, K. & Kilian, M. (2001). Epidemic population structure of Pseudomonas aeruginosa: evidence for a clone that is pathogenic to the eye and that has a distinct combination of virulence factors. Infect Immun 69, 6284-6295.
32. Mahenthiralingam, E., Campbell, M. E., Foster, J., Lam, J. S. & Speert, D. P. (1996). Random amplified polymorphic DNA typing of Pseudomonas aeruginosa isolates recovered from patients with cystic fibrosis. J Clin Microbiol 34, 1129-1135.
33. Ohman, D. E., Burns, R. P. & Iglewski, B. H. (1980). Corneal infections in mice with toxin A and elastase mutants of Pseudomonas aeruginosa. J Infect Dis 142, 547-555.
34. Panagea, S., Winstanley, C., Parsons, Y. N., Walshaw, M. J., Ledson, M. J. & Hart, C. A. (2003). PCR-based detection of a cystic fibrosis epidemic strain of Pseudomonas aeruginosa. Mol Diagn 7, 195-200.
35. Parsons, Y. N., Panagea, S., Smart, C. H., Walshaw, M. J., Hart, C. A. & Winstanley, C. (2002). Use of subtractive hybridization to identify a diagnostic probe for a cystic fibrosis epidemic strain of Pseudomonas aeruginosa. J Clin Microbiol 40, 4607-4611.
36. Pillar, C. M. & Hobden, J. A. (2002). Pseudomonas aeruginosa exotoxin A and keratitis in mice. Invest Ophthalmol Vis Sci 43, 1437-1444.
37. Pirnay, J. P., De Vos, D., Cochez, C., Bilocq, F., Vanderkelen, A., Zizi, M., Ghysels, B. & Cornelis, P. (2002). Pseudomonas aeruginosa displays an epidemic population structure. Environ Microbiol 4, 898-911.
38. Pirnay, J. P., De Vos, D., Cochez, C. & 7 other authors (2003). Molecular epidemiology of Pseudomonas aeruginosa colonization in a burn unit: persistence of a multidrug-resistant clone and a silver sulfadiazine-resistant clone. J Clin Microbiol 41, 1192-1202.
39. Priebe, G. P., Dean, C. R., Zaidi, T. & 7 other authors (2004). The galU gene of Pseudomonas aeruginosa is required for corneal infection and efficient systemic spread following pneumonia but not for infection confined to the lung. Infect Immun 72, 4224-4232.
40. Roy-Burman, A., Savel, R. H., Racine, S., Swanson, B. L., Revadigar, N. S., Fujimoto, J., Sawa, T., Frank, D. W. & Wiener-Kronish, J. P. (2001). Type III protein secretion is associated with death in lower respiratory and systemic Pseudomonas aeruginosa infections. J Infect Dis 183, 1767-1774.
41. Rudner, X. L., Kernacki, K. A., Barrett, R. P. & Hazlett, L. D. (2000). Prolonged elevation of IL-1 in Pseudomonas aeruginosa ocular infection regulates macrophage-inflammatory protein-2 production, polymorphonuclear neutrophil persistence, and corneal perforation. J Immunol 164, 6576-6582.
42. Sato, H., Frank, D. W., Hillard, C. J. & 9 other authors (2003). The mechanism of action of the Pseudomonas aeruginosa-encoded type III cytotoxin, ExoU. EMBO J 22, 2959-2969.
43. Scharfman, A., Arora, S. K., Delmotte, P., Van Brussel, E., Mazurier, J., Ramphal, R. & Roussel, P. (2001). Recognition of Lewis x derivatives present on mucins by flagellar components of Pseudomonas aeruginosa. Infect Immun 69, 5243-5248.
44. Schulert, G. S., Feltman, H., Rabin, S. D., Martin, C. G., Battle, S. E., Rello, J. & Hauser, A. R. (2003). Secretion of the toxin ExoU is a marker for highly virulent Pseudomonas aeruginosa isolates obtained from patients with hospital-acquired pneumonia. J Infect Dis 188, 1695-1706.
45. Schwyn, B. & Neilands, J. B. (1987). Universal chemical assay for the detection and determination of siderophores. Anal Biochem 160, 47-56.
46. Spencer, D. H., Kas, A., Smith, E. E., Raymond, C. K., Sims, E. H., Hastings, M., Burns, J. L., Kaul, R. & Olson, M. V. (2003). Whole-genome sequence variation among multiple isolates of Pseudomonas aeruginosa. J Bacteriol 185, 1316-1325.
47. Thuruthyil, S. J., Zhu, H. & Willcox, M. D. (2001). Serotype and adhesion of Pseudomonas aeruginosa isolated from contact lens wearers. Clin Experiment Ophthalmol 29, 147-149.
48. Vallis, A. J., Yahr, T. L., Barbieri, J. T. & Frank, D. W. (1999). Regulation of ExoS production and secretion by Pseudomonas aeruginosa in response to tissue culture conditions. Infect Immun 67, 914-920.
49. Winstanley, C., Coulson, M. A., Wepner, B., Morgan, J. A. W. & Hart, C. A. (1996). Flagellin gene and protein variation amongst clinical isolates of Pseudomonas aeruginosa. Microbiology 142, 2145-2151.
50. Wolfgang, M. C., Kulasekara, B. R., Liang, X., Boyd, D., Wu, K., Yang, Q., Miyada, C. G. & Lory, S. (2003). Conservation of genome content and virulence determinants among clinical and environmental isolates of Pseudomonas aeruginosa. Proc Natl Acad Sci U S A 100, 8484-8489.
51. Zhang, J., Xu, K., Ambati, B. & Yu, F. S. (2003). Toll-like receptor 5-mediated corneal epithelial inflammatory responses to Pseudomonas aeruginosa flagellin. Invest Ophthalmol Vis Sci 44, 4247-4254.
52. Zolfaghar, I., Evans, D. J. & Fleiszig, S. M. (2003). Twitching motility contributes to the role of pili in corneal infection caused by Pseudomonas aeruginosa. Infect Immun 71, 5389-5393.