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This assumption, that the mixed-orientation film contains equal amounts of cytoplasmic and extracellular orientations, is justified by our observations. First, we have already concluded that our deposition method produces similar coverages for both orientations. Therefore, neither orientation adsorbs preferentially compared to the other, and a random mixture should contain equal amounts of each. Second, the threshold shift observed with mixed orientation correlates with the expectation from a 50/50% mixture. The two oriented depositions cause +2.2 V and -0.4 V of threshold shift. For a 50/50% mixture, we expect a net threshold shift of (1/2)(2.2-0.4) V, or +0.9 V. This value agrees well with the value observed with mixed orientation, 1.0 ±0.2 V. From these two observations, we conclude that the mixed-orientation film is in fact a 50/50% mixture.
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24 which is too weak to explain the charge induced in our devices.
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