Enzyme-coated carbon nanotubes as single-molecule biosensors

Nano Letters

Volumn 3, Issue 6, 2003, Pages 727-730

  Besteman, Koen ^a   Lee, Jeong O ^a   Wiertz, Frank G M ^a   Heering, Hendrik A ^a   Dekker, Cees ^a  

^a DELFT UNIVERSITY OF TECHNOLOGY   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

ENZYME; GLUCOSE; GLUCOSE OXIDASE;

ARTICLE; BIOSENSOR; CONDUCTANCE; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME SUBSTRATE; ENZYME SUBSTRATE COMPLEX; MOLECULE; NANOTECHNOLOGY; OXIDATION REDUCTION REACTION; SEMICONDUCTOR;

EID: 0141521856     PISSN: 15306984     EISSN: None     Source Type: Journal    
DOI: 10.1021/nl034139u     Document Type: Article

References (22)

1. Dekker, C. Phys. Today 1999, 52, 22.
2. McEuen, P. L.; Fuhrer, M. S. Park, H.; IEEE Trans. Nanotechnol. 2002, 1, 78.
3. Tans, S. J.; Verschueren, A. R. M.; Dekker, C. Nature 1998, 393, 49.
4. Kong, J.; Franklin, N. R.; Zhou, C.; Chapline, M. G.; Peng, S.; Cho, K.; Dai, H. Science 2000, 287, 622.
5. Collins, P. G.; Bradley, K.; Ishigami, M.; Zettl, A. Science 2000, 287, 1801.
6. Cui, Y.; Wei, Q.; Park, H.; Lieber, C. M. Science 2001, 293, 1289.
7. Wong, S. S.; Joselevich, E.; Woolley, A. T.; Cheung, C. L.; Lieber, C. M. Nature 1998, 394, 52.
8. Balavoine, F.; Schultz, P.; Richard, C.; Mallouh, V.; Ebbesen, T. W.; Mioskowski, C. Angew. Chem., Int. Ed. Engl. 1999, 38, 1912.
9. Chen, R. J.; Zhang, Y.; Wang, D.; Dai, H. J. Am. Chem. Soc. 2001, 123, 3838.
10. Erlanger, B. F.; Chen, B.-X.; Zhu, M.; Brus, L. Nano Lett. 2001, 1, 465.
11. Shim, M.; Wong Shi Kam, N.; Chen, R. J.; Li, Y.; Dai, H. Nano Lett. 2002, 2, 285.
12. Williams, K. A.; Veenhuizen, P. T. M.; de la Torre, B. G.; Eritja, R.; Dekker, C. Nature 2002, 420, 761.
13. Kong, J.; Soh, H. T.; Cassell, A. M.; Quate, C. F.; Dai, H. Nature 1998, 395, 878.
14. Guo, T.; Nikolaev, P.; Thess, A.; Colbert, D. T.; Smalley, R. E. Chem. Phys. Lett. 1995, 243, 49.
15. Krüger, M.; Buitelaar, M. R.; Nussbaumer, T.; Schönenberger, C. Appl. Phys. Lett. 2001, 78, 1291.
16. Rosenblatt, S.; Yaish, Y.; Park, J.; Gore, J.; Sazonova, V.; McEuen, P. L. Nano Lett. 2002, 2, 869.
17. With SDS-page gel electrophoresis the purity of GOx was verified. Furthermore, GOx was tested for activity in milli-Q water with glucose by monitoring oxygen depletion with a Clark electrode. We did not measure any significant loss of activity after storing the enzyme for 24 h in milli-Q water at ambient temperature.
18. d now depends on the capacitance across the GOx as well as on the double-layer capacitance of the tube-liquid interface. Changes in the liquid composition that only change the tube-liquid double layer capacitance still have as little influence on the overall capacitance as for a bare tube, because the tube-liquid double-layer capacitance is much larger than both quantum capacitance and capacitance across the GOx.
19. Besteman, K. et al., unpublished.
20. d through a change in the tube-liquid double-layer capacitance, one would expect the conductance of the SWNT to be much higher in the 0.1 mM KCl and HCl solutions than in pure milli-Q water because of the higher ionic concentration.
21. Occasionally, in one or two out of eleven devices, a bare semiconducting SWNT did show a similar glucose dependence as well. These one or two devices had a significantly higher noise than the devices shown in Figure 4.
22. 2 basal plane graphite electrodes. Glucose turned out not to increase the capacitance of this carbon electrode. This indicates that a change in the tube-liquid double-layer capacitance as a result of a change in the liquid composition is not the origin of the conductance increase.