Half-of-the-sites binding of reactive intermediates and their analogues to 4-oxalocrotonate tautomerase and induced structural asymmetry of the enzyme

Biochemistry

Volumn 44, Issue 21, 2005, Pages 7725-7737

  Azurmendi, Hugo F ^a   Miller, Scott G ^b   Whitman, Christian P ^b   Mildvan, Albert S ^a  

^a JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b UNIVERSITY OF TEXAS AT AUSTIN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

HALF-SITE STOICHIOMETRY; HOMOHEXAMERIC ENZYMES; HYDROPHOBIC ACTIVE SITES; REACTIVE INTERMEDIATES;

CATALYSIS; HYDROPHOBICITY; MIXTURES; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PHASE EQUILIBRIA; STOICHIOMETRY; SUBSTRATES; TITRATION; X RAY ANALYSIS;

ENZYMES;

2 HYDROXY 2,4 PENTADIENOATE; 4 OXALOCROTONATE TAUTOMERASE; ARGININE; CARBOXYLIC ACID; DICARBOXYLIC ACID; ISOMERASE; MUCONIC ACID; UNCLASSIFIED DRUG;

ARTICLE; BINDING AFFINITY; BINDING SITE; CATALYSIS; CHEMICAL STRUCTURE; ENZYME BINDING; ENZYME INACTIVATION; ENZYME STRUCTURE; ENZYME SUBSTRATE COMPLEX; LIGAND BINDING; MATHEMATICAL ANALYSIS; NITROGEN NUCLEAR MAGNETIC RESONANCE; PRIORITY JOURNAL; PROTEIN INTERACTION; SIGNAL TRANSDUCTION; STOICHIOMETRY; TITRIMETRY; X RAY ANALYSIS;

ADIPIC ACIDS; ARGININE; BINDING SITES; BINDING, COMPETITIVE; CARBOXYLIC ACIDS; DICARBOXYLIC ACIDS; ENZYME INDUCTION; ENZYME INHIBITORS; HYDROGEN; ISOMERASES; MULTIENZYME COMPLEXES; NITROGEN ISOTOPES; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; PROTEIN CONFORMATION; SORBIC ACID; SUBSTRATE SPECIFICITY; TITRIMETRY;

EID: 19644375811     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi0502590     Document Type: Article

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