The carboxy-terminal end of the peptide deformylase from Mycobacterium tuberculosis is indispensable for its enzymatic activity

Biochemical and Biophysical Research Communications

Volumn 332, Issue 2, 2005, Pages 418-425

  Saxena, Rahul ^a   Chakraborti, Pradip K ^a  

^a INSTITUTE OF MICROBIAL TECHNOLOGY   (India)

Author keywords

Gene expression; Iron containing peptide deformylase; Metalloprotease; Mycobacteria; Peptide deformylase

Indexed keywords

ACTINONIN; PEPTIDE DEFORMYLASE; RECOMBINANT PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME KINETICS; ESCHERICHIA COLI; GENE DELETION; GENETIC ANALYSIS; MOLECULAR CLONING; MYCOBACTERIUM TUBERCULOSIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN SYNTHESIS; SEQUENCE ANALYSIS;

AMIDOHYDROLASES; AMINO ACID SUBSTITUTION; ENZYME ACTIVATION; ENZYME STABILITY; HYDROXAMIC ACIDS; MOLECULAR WEIGHT; MUTAGENESIS, SITE-DIRECTED; MYCOBACTERIUM TUBERCULOSIS; OXIDATION-REDUCTION; RECOMBINANT PROTEINS; STRUCTURE-ACTIVITY RELATIONSHIP; SUBSTRATE SPECIFICITY; TEMPERATURE;

CORYNEBACTERINEAE; ESCHERICHIA COLI; MYCOBACTERIUM; MYCOBACTERIUM TUBERCULOSIS; POSIBACTERIA;

EID: 19444368610     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bbrc.2005.04.142     Document Type: Article

References (33)

1. C. Giglione, M. Perrie, and T. Meinnel Peptide deformylase as a target for new generation, broad-spectrum antibacterial agents Mol. Microbiol. 36 2000 1197 1205
2. J. Solbiati, A. Chapman-Smith, J.L. Miller, C.G. Miller, and J.E. Cronan Jr. Processing of the N termini of nascent polypeptide chains requires deformylation prior to methionine removal J. Mol. Biol. 290 1999 607 614
3. D. Mazel, E. Coic, S. Blanchard, and W. Saurin A survey of polypeptide deformylase function throughout the Eubacterial lineage J. Mol. Biol. 266 1997 939 949
4. M.K. Chan, W. Gong, P.T. Rajagopalan, B. Hao, C.M. Tsai, and D. Pei Crystal structure of Escherichia coli peptide deformylase Biochemistry 36 1997 13904 13909
5. E.T. Baldwin, M.S. Harris, A.W. Yem, C.L. Wolfe, A.F. Vosters, K.A. Curry, R.W. Murray, J.H. Bock, V.P. Marshall, J.I. Cialdella, M.H. Merchant, G. Choi, and M.R. Deibel Jr. Crystal structure of type II peptide deformylase from Staphylococcus aureus J. Biol. Chem. 277 2002 31163 31171
6. A. Kreusch, G. Spraggon, C.C. Lee, H. Klock, D. McMullan, K. Ng, T. Shin, J. Vincent, I. Warner, C. Ericson, and S.A. Lesley Structure analysis of peptide deformylases from Streptococcus pneumoniae, Staphylococcus aureus, Thermotoga maritima and Pseudomonas aeruginosa: snapshots of the oxygen sensitivity of peptide deformylase J. Mol. Biol. 30 2003 309 321
7. Z. Zhou, X. Song, Y. Li, and W. Gong Unique structural characteristics of peptide deformylase from pathogenic bacterium Leptospira interrogans J. Mol. Biol. 339 2004 207 215
8. C. Giglione, O. Vallon, and T. Meinnel Control of protein life-span by N-terminal methionine excision EMBO J. 22 2003 13 23
9. P.T. Rajagopalan, A. Datta, and D. Pei Purification, characterization, and inhibition of peptide deformylase from Escherichia coli Biochemistry 36 1997 13910 13918
10. Y. Li, Z. Chen, and W. Gong Enzymatic properties of a new peptide deformylase from pathogenic bacterium Leptospira interrogans Biochem. Biophys. Res. Commun. 295 2002 884 889
11. P.T. Rajagopalan, and D. Pei Oxygen-mediated inactivation of peptide deformylase J. Biol. Chem. 273 1998 22305 22310
12. S.T. Cole, R. Brosch, J. Parkhill, T. Garnier, C. Churcher, D. Harris, S.V. Gordon, K. Eiglmeier, S. Gas, C.E. Barry III, F. Tekaia, K. Badcock, D. Basham, D. Brown, T. Chillingworth, R. Connor, R. Davies, K. Devlin, T. Feltwell, S. Gentles, N. Hamlin, S. Holroyd, T. Hornsby, K. Jagels, A. Krogh, J. McLean, S. Moule, L. Murphy, K. Oliver, J. Osborne, M.A. Quail, M.A. Rajandream, J. Rogers, S. Rutter, K. Seeger, J. Skelton, R. Squares, S. Squares, J.E. Sulston, K. Taylor, S. Whitehead, and B.G. Barrell Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence Nature 393 1998 537 544
13. R. Chaba, M. Raje, and P.K. Chakraborti Evidence that a eukaryotic-type serine/threonine protein kinase from Mycobacterium tuberculosis regulates morphological changes associated with cell division Eur. J. Biochem. 269 2002 1078 1085
14. F.W. Studier, A.H. Rosenberg, J.J. Dunn, and J.W. Dubendroff Use of T7 RNA polymerase to direct expression of cloned genes Methods Enzymol. 185 1990 60 89
15. K. Shirley, Y.C. Sheng, J.S. John, and W. Alice C.W. Dieffenbach G.S. Dveksler PCR Primer: A Laboratory Manual 1995 Cold Spring Harbor Laboratory Cold Spring Harbor, New York 143 155
16. M.M. Bradford A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Anal. Biochem. 72 1976 248 254
17. T. Mizobata, Y. Akiyama, K. Ito, N. Yumoto, and Y. Kawata Effects of the chaperonin GroE on the refolding of tryptophanase from Escherichia coli. Refolding is enhanced in the presence of ADP J. Biol. Chem. 267 1992 17773 17779
18. D. Groche, A. Becker, I. Schlichting, W. Kabsch, S. Schultz, and A.F. Wagner Isolation and crystallization of functionally competent Escherichia coli peptide deformylase forms containing either Iron or Nickel in the active site Biochem. Biophys. Res. Commun. 246 1998 342 346
19. G. Hermanson Bioconjugate techniques 1996 Academic press San Diego, California pp. 112-113
20. J. Sarin, S. Aggarwal, R. Chaba, G.C. Varshney, and P.K. Chakraborti B-subunit of phosphate-specific transporter from Mycobacterium tuberculosis is a thermostable ATPase J. Biol. Chem. 276 2001 44590 44597
21. S.F. Altschul, L.M. Thomas, A.S. Alejandro, Z. Jinghui, Z. Zheng, M. Webb, and J.L. David Gapped BLAST and PSI-BLAST: a new generation of protein database search programs Nucleic Acids Res. 25 1997 3389 3402
22. J.D. Thompson, T.J. Gibson, F. Plewniak, F. Jeanmougin, and D.G. Higgins The CLUSTAL-X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools Nucleic Acids Res. 25 1997 4876 4882
23. H. Kaur, and G.P. Raghava Prediction of beta-turns in proteins from multiple alignment using neural network Protein Sci. 12 2003 627 634
24. C. Han, Q. Wang, L. Dong, H. Sun, S. Peng, J. Chen, Y. Yang, J. Yue, X. Shen, and H. Jiang Molecular cloning and characterization of a new peptide deformylase from human pathogenic bacterium Helicobacter pylori Biochem. Biophys. Res. Commun. 319 2004 1292 1298
25. S.T. Motley, and S. Lory Functional characterization of a serine/threonine protein kinase of Pseudomonas aeruginosa Infect. Immun. 67 1999 5386 5394
26. T. Meinnel, and S. Blanquet Enzymatic properties of Escherichia coli peptide deformylase J. Bacteriol. 177 1995 1883 1887
27. P.T. Rajagopalan, S. Grimme, and D. Pei Characterization of cobalt (II)-substituted peptide deformylase function of the metal ion and the catalytic residue Glu-133 Biochemistry 39 2000 779 790
28. D.Z. Chen, V. Patel, C.J. Hackbarth, W. Wang, G. Dreyer, D.C. Young, P.S. Margolis, C. Wu, Z.J. Ni, J. Trias, R.J. White, and Z. Yuan Actinonin, a naturally occurring antibacterial agent, is a peptide deformylase inhibitor Biochemistry 39 2000 1256 1262
29. J.P. Guilloteau, M. Mathieu, C. Giglione, V. Blanc, A. Dupuy, M. Chevrier, P. Gil, A. Famechon, T. Meinnel, and V. Mikol The crystal structures of four peptide deformylases bound to the antibiotic actinonin reveal two distinct types: a platform for the structure-based design of antibacterial agents J. Mol. Biol. 320 2002 951 962
30. T. Meinnel, C. Lazennec, S. Villoing, and S. Blanquet Structure-function relationship within the peptide deformylase family. Evidence for a conserved architecture of the active site involving three conserved motifs and a metal ion J. Mol. Biol. 267 1997 749 761
31. T. Meinnel, C. Lazennec, and S. Blanquet Mapping of the active site zinc ligands of peptide deformylase J. Mol. Biol. 254 1995 175 183
32. T. Meinnel, C. Lazennec, F. Dardel, J.M. Schmitter, and S. Blanquet The C-terminal domain of peptide deformylase is disordered and dispensable for activity FEBS Lett. 385 1996 91 95
33. M.H. Cynamon, E. Alvirez-Freites, and A.E. Yeo BB-3497, a peptide deformylase inhibitor, is active against Mycobacterium tuberculosis J. Antimicrob. Chemother. 53 2004 403 405