Alterations of intercellular communication in neonatal cardiac myocytes from connexin43 null mice

Cardiovascular Research

Volumn 62, Issue 2, 2004, Pages 397-406

  Vink, Monique J ^a   Suadicani, Sylvia O ^a   Vieira, Delia M ^a   Urban Maldonado, Marcia ^a   Gao, Yang ^a   Fishman, Glenn I ^a,b   Spray, David C ^a  

^a ALBERT EINSTEIN COLLEGE OF MEDICINE   (United States)

^b NEW YORK UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Arrhythmia; Cx43; Electrical coupling; Gap junction; Knockout mice; Nexus

Indexed keywords

CONNEXIN 40; CONNEXIN 43; DYE; MESSENGER RNA;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CELL COMMUNICATION; CELL CULTURE; CHANNEL GATING; ELECTRIC POTENTIAL; FEMALE; GAP JUNCTION; GENOTYPE; HEART BEAT; HEART CONDUCTION; HEART ELECTROPHYSIOLOGY; HEART MUSCLE CELL; HETEROZYGOSITY; IMAGING; MALE; MEASUREMENT; MOUSE; NEWBORN; NONHUMAN; NORTHERN BLOTTING; PRIORITY JOURNAL; PROTEIN EXPRESSION; WESTERN BLOTTING; WILD TYPE;

ANIMALS; ANIMALS, NEWBORN; BLOTTING, NORTHERN; BLOTTING, WESTERN; CELL COMMUNICATION; CELLS, CULTURED; CONNEXIN 43; ELECTROPHYSIOLOGY; GAP JUNCTIONS; IMMUNOHISTOCHEMISTRY; MICE; MICE, KNOCKOUT; MYOCYTES, CARDIAC; RNA, MESSENGER;

EID: 1942470601     PISSN: 00086363     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.cardiores.2004.01.015     Document Type: Article

References (47)

1. Spray D.C., Suadicani S.O., Vink M.J., Srinivas M. Gap junctions in the heart. Sperelakis N., Kurachi Y.et al. Physiology and Pathophysiology of the Heart. 2000;149-172 Academic Press, New York.
2. Saffitz J.E., Davis L.M., Darrow B.J.et al. The molecular basis of anisotropy: role of gap junctions. J. Cardiovasc. Electrophysiol. 6:1995;498-510.
3. Spach M.S., Heidlage J.F. The stochastic nature of cardiac propagation at a microscopic level. Electrical description of myocardial architecture and its application to conduction. Circ. Res. 76:1995;366-380.
4. Beyer E.C., Paul D.L., Goodenough D.A. Connexin43: a protein from rat heart homologous to the gap junction protein from liver. J. Cell Biol. 105:1987;2621-2629.
5. Gros D.B., Jongsma H.J. Connexins in mammalian heart function. Bioessays. 18:1996;719-730.
6. Bastide B., Neyses L., Ganten D., Paul M., Willecke K., Traub O. Gap junction protein connexin40 is preferentially expressed in vascular endothelium and conductive bundles of rat myocardium and is increased under hypertensive conditions. Circ. Res. 73:1993;1138-1149.
7. Davis L.M., Kanter H.L., Beyer E.C., Saffitz J.E. Distinct gap junction protein phenotypes in cardiac tissues with disparate conduction properties. J. Am. Coll. Cardiol. 24:1994;1124-1132.
8. Delorme B., Dahl E., Jarry-Guichard T.et al. Developmental regulation of connexin40 gene expression in mouse heart correlates with the differentiation of the conduction system. Dev. Dyn. 204:1995;358-371.
9. De Maziere A., Analbers L., Jongsma H.J., Gros D. Immunoelectron microscopic visualization of the gap junction protein connexin40 in the mammalian heart. Eur. J. Morphol. 31:1993;51-54.
10. Gros D., Jarry-Guichard T., Ten Velde I.et al. Restricted distribution of connexin40, a gap junctional protein in mammalian heart. Circ. Res. 74:1994;839-851.
11. Coppen S.R., Kodama I., Boyett M.R.et al. Connexin45, a major connexin of the rabbit sinoatrial node, is co-expressed with Connexin43 in a restricted zone at the nodal-crista terminalis border. J. Histochem. Cytochem. 47:1999;907-918.
12. Coppen S.R., Severs N.J., Gourdie R.G. Connexin45 (alpha6) expression delineates an extended conduction system in the embryonic and mature rodent heart. Dev. Genet. 24:1999;82-90.
13. Reed K.E., Westphale E.M., Larson D.M., Wang H.-Z., Veenstra R.D., Beyer E.C. Molecular cloning and functional expression of human connexin37, an endothelial cell gap junction protein. J. Clin. Invest. 91:1993;997-1004.
14. Van Rijen H.V.M., vanKempen M.J.A., Analbers L.V.S.et al. Gap junctions in human umbilical cord endothelial cells contain multiple connexins. Am. J. Physiol. 272:1997;C117-C130.
15. Dong Y., Ross M., Gruyters T.et al. Differential expression of two gap junction proteins in corneal epithelium. Eur. J. Cell. Biol. 64:1994;95-100.
16. Bruzzone R., Haefliger J.A., Gimlich R.L., Paul D.L. Conenxin40, a component of gap junctions in vascular endothelium, is restricted in its ability to interact with other connexins. Mol. Biol. Cell. 4:1993;7-20.
17. Elfang C., Eckert R., Lichtenberg-Frate H.et al. Specific permeability and selective formation of gap junction channels in connexin-transfected HeLa cells. J. Cell Biol. 129:1995;805-817.
18. Valiunas V., Weingart R., Brink P.R. Formation of heterotypic gap junction channels by connexins 40 and 43. Circ. Res. 86:2000;E42-E49.
19. Moreno A.P., Saez J.C., Fishman G.I., Spray D.C. Human connexin43 gap junction channels. Regulation of unitary conductances by phosphorylation. Circ. Res. 74:1994;1050-1057.
20. Kwak B.R., Saez J.C., Wilders R.et al. Effects of cGMP-dependent phosphorylation on rat and human connexin43 gap junction channels. Pflugers Arch. 430:1995;770-778.
21. Takens-Kwak B.R., Jongsma H.J. Cardiac gap junctions: three distinct single channel conductances and their modulation by phosphorylating treatments. Pflugers Arch. 422:1992;198-200.
22. Moreno A.P., Fishman G.I., Beyer E.C., Spray D.C. Voltage dependent gating and single channel analysis of heterotypic gap junction channels formed of Cx45 and Cx43. Prog. Cell Res. 4:1995;405-408.
23. Veenstra R.D., Wang H.Z., Beblo D.A., Chilton M.G., Harris A.L., Beyer E.C.et al. Selectivity of connexin-specific gap junctions does not correlate with channel conductance. Circ. Res. 77:1995;1156-1165.
24. Reaume A.G., de Sousa P.A., Kulkarni S.et al. Cardiac malformation in neonatal mice lacking connexin43. Science. 267(5205):1995;1831-1834.
25. Gutstein D.E., Morley G.E., Tamaddon H.et al. Conduction slowing and sudden arrhythmic death in mice with cardiac-restricted inactivation of connexin43. Circ. Res. 88:2001;333-339.
26. Dermietzel R., Gao Y., Scemes E.et al. Connexin43 null [Cx43(-/-)] mice reveal that astrocytes express multiple connexins. Br. Res. Rev. 32:2000;45-56.
27. 2+signaling in wildtype and in Cx43-null neonatal mouse cardiac myocytes. J. Am. Physiol. 279:2000;H3076-H3088.
28. Yamamoto T., Ochalski A., Hertzberg E.L., Nagy J.I. LM and EM immunolocalization of the gap junctional protein connexin43 in rat brain. Brain Res. 508:1990;313-319.
29. Srinivas M., Costa M., Gao Y., Fort A., Fishman G.I., Spray D.C. Voltage dependence of macroscopic and unitary currents of gap junction channels formed by mouse connexin50 expressed in rat neuroblastoma cells. J. Physiol. 517:1999;673-689.
30. Burt J.M., Spray D.C. Volatile anesthetics block intercellular communication between neonatal rat myocardial cells. Circ. Res. 65:1989;829-837.
31. Guerrero P.A., Schuessler R.B., Davis L.M.et al. Slow ventricular conduction in mice heterozygous for a Connexin43 null mutation. J. Clin. Invest. 99:1997;1991-1998.
32. Morley G.E., Vaidya D., Samie F.H., Lo C., Delmar M., Jalife J. Characterization of conduction in the ventricles of normal and heterozygous Cx43 knockout mice using optical mapping. J. Cardiovasc. Electrophysiol. 10:1999;1361-1375.
33. Delmar M., Michaels D.C., Johnson T., Jalife J. Effects of increasing intracellular resistance on transverse and longitudinal propagation in sheep epicardial muscle. Circ. Res. 60:1987;780-785.
34. Yao J.-A., Gutstein D.E., Liu F., Fishman G.I., Wit A.L. Cell coupling between ventricular myocyte pairs from connexin43-deficient murine hearts. Circ. Res. 93:2003;736-743.
35. Thomas S.P., Kucera J.P., Bircher-Lehmann L., Rudy Y., Saffitz J.E., Kleber A.G. Impulse propagation in synthetic strands of neonatal cardiac myocytes with genetically reduced levels of connexin43. Circ. Res. 92:2003;1209-1216.
36. Ai Z., Fischer A., Spray D.C., Brown A.M., Fishman G.I. Wnt-1 regulation of connexin43 in cardiac myocytes. J. Clin. Invest. 105:2000;161-171.
37. Moreno A.P., Rook M.B., Fishman G.I., Spray D.C. Gap junction channels: distinct voltage-sensitive and -insensitive conductance states. Biophys J. 67:1994;113-119.
38. Hellmann P., Winterhager E., Spray D.C. Properties of Connexin40 gap junction channels endogenously expressed and exogenously overexpressed in human choriocarcinoma cell lines. Eur. J. Physiol. 432:1996;501-509.
39. Bukauskas F.F., Elfgang C., Willecke K., Weingart R. Biophysical properties of gap junction channels formed by mouse connexin40 in induced pairs of transfected human HeLa cells. Biophys. J. 68:1995;2289-2298.
40. 3. J. Gen. Physiol. 109:1997;509-522.
41. Haubrich S., Schwarz H.J., Bukauskas F.et al. Incompatibility of connexin 40 and 43 hemichannels in gap junctions between mammalian cells is determined by intracellular domains. Mol. Biol. Cell. 7:1996;1995-2006.
42. Polontchouk L.O., Valiunas V., Haefliger J.-A., Eppenberger H.M., Weingart R. Expression and regulation of connexins in cultured ventricular myocytes isolated from adult rat hearts. Pflugers Arch. 443:2002;676-689.
43. Li X., Simard J.M. Multiple connexins form gap junction channels in rat basilar artery smooth muscle cells. Circ. Res. 84:1999;1277-1284.
44. Elenes S., Rubart M., Moreno A.P. Junctional communication between isolated pairs of canine atrial cells is mediated by homogeneous and heterogeneous gap junction channels. J. Cardiovasc. Electrophysiol. 10:1999;990-1004.
45. He D.S., Jiang J.X., Taffet S.M., Burt J.M. Formation of heteromeric gap junction channels by connexins 40 and 43 in vascular smooth muscle cells. Proc. Natl. Acad. Sci. U. S. A. 96:1999;6495-6500.
46. Lo C.W., Cohen M.F., Huang G.Y.et al. Cx43 gap junction gene expression and gap junctional communication in mouse neural crest cells. Dev. Genet. 20:1997;119-132.
47. Ewart J.L., Cohen M.F., Meyer R.A.et al. Heart and neural tube defects in transgenic mice overexpressing the Cx43 gap junction gene. Development. 24:1997;1281-1292.