Properties of connexin40 gap junction channels endogenously expressed and exogenously overexpressed in human choriocarcinoma cell lines

Pflugers Archiv European Journal of Physiology

Volumn 432, Issue 3, 1996, Pages 501-509

  Hellmann, Peter ^a   Winterhager, Elke ^a   Spray, David C ^b  

^a UNIVERSITY OF DUISBURG ESSEN   (Germany)

^b ALBERT EINSTEIN COLLEGE OF MEDICINE   (United States)

Author keywords

Connexins; Intercellular coupling; Ion channels; Tissue invasion; Trophoblast

Indexed keywords

GAP JUNCTION PROTEIN; ION CHANNEL;

ARTICLE; CELL JUNCTION; CHORIOCARCINOMA; CONTROLLED STUDY; GENE EXPRESSION; HUMAN; HUMAN CELL; ION CONDUCTANCE; NORTHERN BLOTTING; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; TROPHOBLAST;

ANIMALS; BASE SEQUENCE; BLOTTING, NORTHERN; CHORIOCARCINOMA; CONNEXINS; DNA PROBES; GAP JUNCTIONS; HUMANS; IMMUNOHISTOCHEMISTRY; MICE; MOLECULAR SEQUENCE DATA; PATCH-CLAMP TECHNIQUES; POLYMERASE CHAIN REACTION; RATS; RNA, MESSENGER; TUMOR CELLS, CULTURED;

EID: 0030017572     PISSN: 00316768     EISSN: None     Source Type: Journal    
DOI: 10.1007/s004240050162     Document Type: Article

References (47)

1. Bastide B, Neyses L, Ganten D, Paul M, Willecke K, Trauh O (1993) Gap junction protein connexin40 is preferentially expressed in vascular endothelium and conductive bundles of rat myocardium and is increased under hypertensive conditions. Circ Res 73:1138-1149
2. Beblo DA, Wang H-Z, Beyer EC, Westphale E, Veenstra RD (1995) Unique conductance, gating and selective permeability properties of gap junction channels formed by connexin40. Circ Res 77:813-822
3. Beyer EC, Paul L. Goodenough DA (1987). Connexin43: a protein from rat heart homologous to a gap junction protein from liver. J Cell Biol 105:2621-2629
4. Bruzzone R, Haeflinger J, Gimlich R, Paul D (1993) Connexin40, a component of gap junctions in vascular endothelium, is restricted in its ability to interact with other connexins. Mol Biol Cell 4:7-20
5. Bukauskas FF, Elfgang C, Willecke K, Weingart R (1995) Biophysical properties of gap junction channels formed by mouse connexin40 in induced pairs of transfected human HeLa cells. Biophys J 68:2289-2298
6. Burres NS, Cass CE (1986). The effects of hypoxanthine on methotrexate-induced differentiation of cultured human choriocarcinoma (BeWo) cells. Biochem Cell Biol 64:811-815
7. Burt JM, Spray DC (1989) Volatile anesthetics block intercellular communication between neonatal rat myocardial cells. Circ Res 65:829-837
8. Cross JC, Werb Z, Fisher SJ (1994) Implantation and the placenta. Key pieces of the development puzzle. Science 266: 1508-1518
9. Elfgang C, Eckert R, Lichtenberg-Frat H, Butterweck A, Trauh A, Klein RA, Huelser D, Willecke K (1995) Specific permeability and selective formation of gap junction channels in connexin-transfected HeLa Cells. J Cell Biol 129:805-817
10. Gabriels JE, Paul DL (1993) Characterization of connexin expression in rat aortic endothelial cells during in vitro wounding. Mol Biol Cell 4:329a
11. Gros D, Jarry-Guichard T, Velde IT, Maziere A, van Kempen MJA, Davoust J, Briand JP, Moorman AFM, Jongsma HJ (1994) Restricted distribution of connexin40, a gap junction protein, in mammalian heart. Circ Res 74:839-851
12. Gruemmer R, Hohn H-P, Marcel MM. Denker H-W (1994) Adhesion and invasion of three human choriocarcinoma cell lines into human endometrium in a three-dimensional organ culture system. Placenta 15:411-429
13. Haefliger J, Bruzzone R, Jenkins N, Gilbert D, Copeland N, Paul D (1992) Four novel members of the connexin family of gap junction proteins. J Biol Chem 267:2057-2064
14. Hellmann P, von Ostau C, Gruemmer R, Winterhager E (1995) Connexin40 expression in the human trophoblast: implicator for proliferation and invasion properties. Placenta 16:A26
15. Hennemann H, Sychyna T, Jungbluth S, Dahl E, Schwarz J, Nicholson B, Willecke K (1992) Molecular cloning and functional expression of mouse connexin40, a second gap junction gene preferentially expressed in lung. J Cell Biol 117:1299-1310
16. Hohn H-P, Boots LR, Denker H-W, Hock M (1993) Differentiation of human trophoblast cells in vitro stimulated by extracellular matrix. Troph Res 7:181-200
17. John NJ, Linke M, Denker H-W (1993) Retinoic acid decreases attachment of JAr choriocarcinoma spheroids to a human endometrial cell monolayer in vitro. Placenta 14:13-24
18. Kanter HL, Laing JG, Beyer EC, Green KG, Saffitz JE (1993) Multiple connexins colocalized in canine ventricular myocyte gap junctions. Circ Res 73:344-350
19. Kanter HL, Saffitz JE, Beyer EC (1994) Molecular cloning of two human cardiac gap junction proteins, connexin40 and connexin45. J Mol Cell Cardiol 26:861-868
20. Kohler PO, Bridson E (1971) Isolation of hormone-producing clonal lines of human choriocarcinoma. J Clin Endocrinol 32:683-687
21. Laing JG, Westphale EM, Engelmann GL, Beyer EC (1994) Characterisation of the gap junction protein, connexin45. J Membr Biol 139:31-40
22. Little TL, Beyer EC, Duling BR (1995) Connexin43 and connexin40 gap junction proteins are present in arteriolar smooth muscle and endothelium in vivo. Am J Physiol 268:H729-H739
23. Moore LK, Burt JM (1994) Selective block of gap junction channel expression with connexin-specific antisense oligodeoxynucleotides. Am J Physiol 267:C1371-C1380
24. Moos M, Gallwitz D (1983) Structure of two human b-actin-related processed genes one of which is located next to simple repetitive sequence. EMBO J 2:757-761
25. Moreno AP, Suez JC, Fishman GI, Spray DC (1994) Gap junction channels: distinct voltage-sensitive and insensitive conductance states. Biophys J 67:113-119
26. Moreno AP, Saez JC, Fishman GI, Spray DC (1994) Human connexin43 gap junction channels: regulation of unitary conductances by phosphorylation. Circ Res 74:1050-1057
27. Moreno AP, Laing JG, Beyer EC, Spray DC (1995) Properties of gap junction channels formed of connexin45 endogenously expressed in human hepatoma (SKHep1) cells. Am J Physiol 268:C356-C365
28. Neyton J, Trautmann A (1985) Single channel currents of an intracellular junction. Nature 357:331-335
29. Ohlsson R (1989) Growth factors, protooncogenes and human placental development. Cell Diff Dev 28:1-7
30. Pattillo RA, Gey GO (1968) The establishment of a cell line of human hormone synthesizing trophoblastic cells in vitro. Cancer Res 28:1231-1236
31. Pattillo RA, Ruckert A, Hussa R, Bernstein R, Delfs E (1971) The JAr cell line-continuous human multihormone production and controls (abstract 101) In Vitro 6:368
32. Pattillo RA, Gey GO, Delfs E, Huang WY, Hause L, Garancis J, Knoth M, Amatruda J, Bertino J, Friesen HG, Mattingly RF (1971) The hormone-synthesizing trophoblastic cell in vitro: a model for cancer research and placental hormone synthesis. Ann NY Acad Sci 172:288-298
33. Pattillo RA, Ruckert A, Hussa RO, Huang WY, Delfs E, Mattingly RF (1972) Estrogen production by trophoblastic tumors in tissue culture. J Clin Endocrinol 34:59-61
34. Paul DL (1986) Molecular cloning of cDNA for rat liver gap junction protein. J Cell Biol 103:123-134
35. Spray DC (1994) Physiological and pharmacological regulation of gap junction channels. In: Citi S (ed) Molecular mechanisms of epithelial cell junctions: from development to disease. Medical Intelligence Unity, RG Landes, Austin, Tex., pp 195-215
36. Spray DC, Harris AL, Bennett MVL (1981) Equilibrium properties of a voltage dependent junctional conductance. J Gen Physiol 77:75-94
37. Strickland S, Richards WG (1992) Invasion of the trophoblast. Cell 71:355-357
38. Traub O, Eckert R, Lichtenberg-Frate H, Elfgang C, Bastide B, Scheidtmann K, Huelser D, Willecke K (1994) Immunochemical and electrophysiological characterization of murine connexin40 and -43 expressed in mouse tissue and transfected human cells. Eur J Cell Biol 64:101-112
39. White RL, Spray DC, Campos de Carvalho AC, Wittenberg BA, Bennett MVL (1985) Some physiological and pharmacological properties of gap junctions between cardiac myocytes dissociated from adult rat. Am J Physiol:C447-C455
40. White TE, Saltzman RA, DiSant Agnese PA, Keng PC, Sutherland RM, Miller RK (1988) Human choriocarcinoma (JAr) cells grown as multicellular spheroids. Placenta 9: 583-598
41. Willecke K (1993) The mouse connexin gene family. In: Hall JE, Zampighi GA, Davis M (eds) Progress in cell research, vol. 4. Elsevier, Amsterdam, pp 33-37
42. Willecke K, Hennemann H, Dahl E, Jungbluth S, Heynkes R (1991) The diversity of connexin genes encoding gap junctional proteins. Eur J Cell Biol 56:1-7
43. Willecke K, Heynkes R, Dahl E, Stutenkemper R, Hennemann H, Jungbluth S, Sychyna T, Nicholson BJ (1991) Mouse connexin37: cloning and functional expression of a gap junction gene highly expressed in lung. J Cell Biol 177: 1299-1310
44. Winterhager E, Bruemmer F, Dermietzel R, Huelser DF, Denker H-W (1988) Gap junction formation in rabbit uterine epithelium in response to embryo recognition. Dev Biol 126:203-211
45. Winterhager E, Stutenkemper R, Traub O, Beyer E, Willecke K (1991) Expression of different connexin genes in rat uterus during decidualization and at term. Eur J Cell Biol 55:133-142
46. Yamasaki H (1991) Aberrant expression and function of gap junctions during carciogenesis. Environ Health Perspect 93: 191-197
47. Zhang JT, Nicholson BJ (1989) Sequence and tissue distribution of a second protein of hepatic gap junctions, Cx26, as deduced from its cDNA. J Cell Biol 109:3391-3401