Connexin45, a major connexin of the rabbit sinoatrial node, is co- expressed with connexin43 in a restricted zone at the nodal-crista terminalis border

Journal of Histochemistry and Cytochemistry

Volumn 47, Issue 7, 1999, Pages 907-918

  Coppen, Steven R ^a   Kodama, Itsuo ^b   Boyett, Mark R ^c   Dobrzynski, Halina ^c   Takagishi, Yoshiko ^b   Honjo, Haruo ^b   Yeh, Hung I ^a,d   Severs, Nicholas J ^a,e  

^a NATIONAL HEART AND LUNG INSTITUTE   (United Kingdom)

^b NAGOYA UNIVERSITY   (Japan)

^c UNIVERSITY OF LEEDS   (United Kingdom)

^d MACKAY MEMORIAL HOSPITAL   (Taiwan)

^e ROYAL BROMPTON HOSPITAL   (United Kingdom)

Author keywords

Confocal microscopy; Connexins; Gap junctions; Heart; Sinoatrial node

Indexed keywords

CONNEXIN 40; CONNEXIN 43; CONNEXIN 45; GAP JUNCTION PROTEIN; UNCLASSIFIED DRUG;

ANIMAL TISSUE; ARTICLE; CELL COMMUNICATION; IMMUNOCYTOCHEMISTRY; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; RABBIT; SINUS NODE; TISSUE DISTRIBUTION;

ANIMALIA; ORYCTOLAGUS CUNICULUS; TERMINALIS;

EID: 0033033946     PISSN: 00221554     EISSN: None     Source Type: Journal    
DOI: 10.1177/002215549904700708     Document Type: Article

References (35)

1. Anumonwo JMB, Wang H-Z, Trabka-Janik E, Dunham B, Veenstra RD, Delmar M, Jalife J (1992) Gap junctional channels in adult mammalian sinus nodal cells: immunolocalization and electrophysiology. Circ Res 71:229-239
2. Beblo DA, Wang HZ, Beyer EC, Westphale EM, Veenstra RD (1995) Unique conductance, gating, and selective permeability properties of gap junction channels formed by connexin40. Circ Res 77:813-822
3. Bleeker WK, Mackaay AJC, Masson-Pevet M, Bouman LN, Becker AE (1980) Functional and morphological organization of the rabbit sinus node. Circ Res 46:11-22
4. Bonke FIM (1973) Passive electrical properties of atrial fibres of the rabbit heart. Pflugers Arch 339:1-15
5. Bouman LN, Duivenvoorden JJ, Bukauskas FF, Jongsma HJ (1989) Anisotropy of electronus in the sinoatrial node of the rabbit heart. J Mol Cell Cardiol 21:407-418
6. Bruzzone R, White TW, Paul DL (1996) Connections with connexins: the molecular basis of direct intercellular signaling. Eur J Biochem 238:1-27
7. Bukauskas FF, Elfgang C, Willecke K, Weingart R (1995) Biophysical properties of gap junction channels formed by mouse connexin40 in induced pairs of transfected human HeLa cells. Biophys J 68:2289-2298
8. Coppen SR, Dupont E, Rothery S, Severs NJ (1998) Connexin45 expression is preferentially associated with the ventricular conduction system in mouse and rat heart. Circ Res 82:232-243
9. Davis LM, Kanter HL, Beyer EC, Saffitz JE (1994) Distinct gap junction protein phenotypes in cardiac tissues with disparate conduction properties. J Am Coll Cardiol 24:1124-1132
10. Davis LM, Rodefeld ME, Green K, Beyer EC, Saffitz JE (1995) Gap junction protein phenotypes of the human heart and conduction system. J Cardiovasc Electrophysiol 6:813-822
11. De Mazière AMGL, Van Ginneken ACG, Wilders R, Jongsma HJ, Bouman LN (1992) Spatial and functional relationship between myocytes and fibroblasts in the rabbit sinoatrial node. J Mol Cell Cardiol 24:567-578
12. Gros DB, Jongsma HJ (1996) Connexins in mammalian heart function. BioEssays 18:719-730
13. Joyner RW, Van Capelle FJL (1986) Propagation through electrically coupled cells: how a small SA node drives a large atrium. Biophys J 50:1157-1164
14. Koval M, Geist ST, Westphale EM, Kemendy AE, Civitelli R, Beyer EC, Steinberg TH (1995) Transfected connexin45 alters gap junction permeability in cells expressing endogenous connexin43. J Cell Biol 130:987-995
15. Kumar NM, Gilula NB (1996) The gap junction communication channel. Cell 84:381-388
16. Kwong KF, Schuessler RB, Green KG, Laing JG, Beyer EC, Boineau JP, Saffitz JE (1998) Differential expression of gap junction proteins in the canine sinus node. Circ Res 82:604-612
17. Masson-Pevet M, Bleeker WK, Gros D (1979) The plasma membrane of leading pacemaker cells in the rabbit sinus node. A qualitative and quantitative ultrastructural analysis. Circ Res 45: 621-623
18. Moreno AL, Laing JG, Beyer EC, Spray DC (1995) Properties of gap junction channels formed of connexin 45 endogenously expressed in human hepatoma (SKHep1) cells. Am J Physiol 268:C356-365
19. Oosthoek PW, Virágh S, Mayen AEM, Van Kempen MJA, Lamers WH, Moorman AFM (1993) Immunohistochemical delineation of the conduction system: I: The sinoatrial node. Circ Res 73: 473-481
20. Opthof T (1994) Gap junctions in the sinoatrial node: immunohistochemical localization and correlation with activation patterns. J Cardiovasc Electrophysiol 5:138-143
21. Saffitz JE, Davis LM, Darrow BJ, Kanter HL, Laing JG, Beyer EC (1995) The molecular basis of anisotropy: role of gap junctions. J Cardiovasc Electrophysiol 6:498-510
22. Saffitz JE, Kanter HL, Green KG, Tolley TK, Beyer EC (1994) Tissue-specific determinants of anisotropic conduction velocity in canine atrial and ventricular myocardium. Circ Res 74:1065-1070
23. Severs NJ, Dupont E, Kaprielian RR, Yeh H-I, Rothery S (1996) Gap junctions and connexins in the cardiovascular system. In Yacoub MH, Carpentier A, Pepper J, Fabiani J-N, eds. Annual of Cardiac Surgery 1996. 9th ed. London, Current Science, 31-44
24. ten Velde I, De Jonge B, Verheijck EE, Van Kempen MJA, Analbers L, Gros D, Jongsma HJ (1995) Spatial distribution of connexin43, the major cardiac gap junction protein, visualizes the cellular network for impulse propagation from sinoatrial node to atrium. Circ Res 76:802-811
25. ten Velde I, De Mazière AMGL, De Jonge B, Gros D, Jongsma HJ (1992) Gap junction distribution in and around sinoatrial node studied by immunohistochemistry. Histochem J 24:575-576
26. Trabka-Janik E, Coombs W, Lemanski LF, Delmar M, Jalife J (1994) Immunohistochemical localization of gap junction protein channels in hamster sinoatrial node in correlation with electrophysiologic mapping of the pacemaker region. J Cardiovasc Electrophysiol 5:125-127
27. Traub O, Eckert R, Lichtenberg-Fraté H, Elfgang C, Bastide B, Scheidtmann KH, Hülser DF, Willecke K (1994) Immunochemical and electrophysiological characterization of murine connexin40 and -43 in mouse tissues and transfected human cells. Eur J Cell Biol 64:101-112
28. Van Kempen MJA, Fromaget C, Gros D, Moorman AFM, Lamers WH (1991) Spatial distribution of connexin43, the major cardiac gap junction protein, in the developing and adult rat heart. Circ Res 68:1638-1651
29. Veenstra RD, Wang H-Z, Beyer EC, Brink PR (1994) Selective dye and ionic permeability of gap junction channels formed by connexin45. Circ Res 75:483-490
30. White TW, Bruzzone R (1996) Multiple connexin proteins in single intercellular channels: connexin compatibility and functional consequences. J Bioenerg Biomembr 28:339-350
31. White TW, Bruzzone R, Goodenough DA, Paul DL (1994) Voltage gating of connexins. Nature 371:208-209
32. White TW, Paul DL, Goodenough DA, Bruzzone R (1995) Functional analysis of selective interactions among rodent connexins. Mol Biol Cell 6:459-470
33. Winslow RL, Jongsma HJ (1995) Role of tissue geometry and spatial localization of gap junctions in generation of the pacemaker potential. J Physiol 487P:126P
34. Yamamoto M, Honjo H, Niwa R, Kodama I (1998) Low frequency extracellular potentials recorded from the sinoatrial node. Cardiovasc Res 39:360-370
35. Yeh H-I, Dupont E, Rothery S, Coppen SR, Severs NJ (1998) Individual gap junction plaques contain multiple connexins in arterial endothelium. Circ Res 83:1248-1263