Structure of epitopes recognized by the antibodies to α(181-192) peptides of neuronal nicotinic acetylcholine receptors: Extrapolation to the structure of acetylcholine-binding domain

Journal of Neuroimmunology

Volumn 121, Issue 1-2, 2001, Pages 59-66

  Skok, M ^a   Lykhmus, E ^a   Bobrovnik, S ^a   Tzartos, S ^b   Tsouloufis, T ^b   Vanderesse, R ^c   Coutrot, F ^c   Thong Cung, M ^c   Marraud, M ^c   Krikorian, D ^d   Sakarellos Daitsiotis, M ^d  

^a PALLADIN INSTITUTE OF BIOCHEMISTRY   (Ukraine)

^b HELLENIC PASTEUR INSTITUTE   (Greece)

^c UNIVERSITÉ DE LORRAINE   (France)

^d UNIVERSITY OF IOANNINA   (Greece)

Author keywords

Acetylcholine binding site; Antibodies; Epitopes; Nicotinic acetylcholine receptor; NMR; Synthetic peptides

Indexed keywords

ACETYLCHOLINE; ALANINE; AMINO ACID; EPITOPE; MONOCLONAL ANTIBODY; NICOTINIC RECEPTOR; PEPTIDE DERIVATIVE; PROTEIN SUBUNIT;

AMINO ACID SUBSTITUTION; ARTICLE; MOLECULAR MODEL; NUCLEAR MAGNETIC RESONANCE; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN STABILITY; PROTEIN STRUCTURE;

ALANINE; AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ANIMALS; ANTIBODIES, MONOCLONAL; ANTIBODY SPECIFICITY; BINDING SITES; EPITOPES; MAGNETIC RESONANCE SPECTROSCOPY; MODELS, MOLECULAR; NEURONS; PEPTIDE FRAGMENTS; PROTEIN STRUCTURE, TERTIARY; RECEPTORS, NICOTINIC;

EID: 19244373912     PISSN: 01655728     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0165-5728(01)00437-4     Document Type: Article

References (37)

1. Nicotinic receptor function in the mammalian nervous system
2. Diversity of nicotinic acetylcholine receptors in the brain: V. α-Bungarotoxin-sensitive nicotinic receptors in olfactory bulb neurons and presynaptic modulation of glutamate release
3. Localization of agonist and competitive antagonist binding sites on nicotinic acetylcholine receptors
4. NMR solution structure of an alpha-bungarotoxin/nicotinic receptor peptide complex
5. Crystal structure of an ACh-binding protein reveals the ligand-binding domain of nicotinic receptors
6. Activation and blockade of mouse muscle nicotinic channels by antibodies directed against the binding site of the acetylcholine receptor
7. The two-dimensional transferred nuclear Overhauser effect: Theory and practice
8. Theory and applications of the transferred nuclear Overhauser effect to the study of the conformations of small ligands bound to proteins
9. Theory of the time dependent transferred nuclear Overhauser effect: Applications to structural analysis of ligand-protein complexes in solution
10. The α5 gene product assembles with multiple acetylcholine receptor subunits to form distinctive receptor subtypes in brain
11. Mapping of a cholinergic binding site by means of synthetic peptides, monoclonal antibodies and α-bungarotoxin
12. Critical elements determining diversity in agonist binding and desensitization of neuronal nicotinic acetylcholine receptor
13. Nicotinic receptors at the amino acid level
14. Pharmacological and functional diversity of neuronal nicotinic acetylcholine receptors
15. + nicotinamide but does not experience a specific hydride transfer
16. A monoclonal antibody interfering with binding and response of the acetylcholine receptor
17. Measurement of the true affinity constant in solution of antigen-antibody complexes by enzyme-linked immunosorbent assay
18. Expression and spectroscopic analysis of soluble nicotinic acetylcholine receptor fragments derived from the extracellular domain of the alpha-subunit
19. Analysis of exposed regions on the main extracellular domain of mouse acetylcholine receptor α subunit in live muscle cells by binding profiles of antipeptide antibodies
20. The 3D-structure of the complex between an Fv antibody fragment and an analogue of the main immunogenic region of the acetylcholine receptor: A combined 2D-NMR, homology and molecular modeling approaches
21. Improved secondary predictions of a nicotinic receptor subunit: Incorporation of solvent accessibility and experimental data into a two-dimensional representation
22. Nicotinic binding to native and substituted peptides comprising residues 188-207 of nicotinic acetylcholine receptor alpha1, alpha2, alpha3, alpha4, alpha5 and alpha7 subunits
23. Chimeric analysis of a neuronal nicotinic acetylcholine receptor reveals amino acids conferring sensitivity to alpha-bungarotoxin
24. Neuronal nicotinic acetylcholine receptors
25. Nicotinic acetylcholine receptors: Structure, function and main immunogenic region
26. Structural determinants within residues 180-199 of the rodent alpha5 nicotinic acetylcholine receptor subunit involved in α-bungarotoxin binding
27. 3H] nicotine as an agonist photoaffinity label
28. Nicotinic acetylcholine receptor at 4.6 Å resolution: Transverse tunnels in the channel wall
29. 76] MIR analogue: A molecular dynamics simulation from two-dimensional NMR data
30. Gradient-tailored excitation for single-quantum NMR spectroscopy of aqueous solutions
31. Monoclonal antibodies directed against a synthetic peptide corresponding to the α-bungarotoxin binding region of the acetylcholine receptor
32. Alpha subunit composition of nicotinic acetylcholine receptors in the rat autonomic ganglia neurons as determined with subunit-specific anti-α(181-192) peptide antibodies
33. Probing the agonist domain of the nicotinic acetylcholine receptor by cysteine scanning mutagenesis reveals residues in proximity to the alpha-bungarotoxin binding site
34. Histidine 186 of the nicotinic acetylcholine receptor alpha subunit requires the presence of the 192-193 disufide bridge to interact with alpha-bungarotoxin
35. Acetylcholine receptor channel imaged in the open state
36. Nicotinic acetylcholine receptor subtypes in rat superior cervical ganglion neurons as studied by sequential application of two α subunit-specific antibodies
37. The solution structure of the complex formed between alpha-bungarotoxin and an 18-mer cognate peptide derived from the alpha 1 subunit of the nicotinic acetylcholine receptor from Torpedo californica