Generation of a strong promoter for Escherichia coli from eukaryotic genome DNA

Journal of Biotechnology

Volumn 115, Issue 3, 2005, Pages 239-248

  Kagiya, Go ^a   Ogawa, Ryohei ^a,b   Hatashita, Masanori ^a   Takagi, Keiichi ^a   Kodaki, Tsutomu ^c   Hiroishi, Shingo ^d   Yamamoto, Kazutaka ^a  

^a WAKASA WAN ENERGY RESEARCH CENTER   (Japan)

^b TOYAMA MEDICAL AND PHARMACEUTICAL UNIVERSITY   (Japan)

^c KYOTO UNIVERSITY   (Japan)

^d FUKUI PREFECTURAL UNIVERSITY   (Japan)

Author keywords

Directed evolution; Error prone PCR; Expression; Promoter; Regulation

Indexed keywords

DNA; ENZYMES; GENES; MANGANESE COMPOUNDS; MUTAGENESIS; REACTION KINETICS;

DIRECTED EVOLUTION; ERROR PRONE PCR; EXPRESSION; PROMOTER; REGULATION;

ESCHERICHIA COLI;

BETA GALACTOSIDASE; DNA FRAGMENT; GENOMIC DNA; LUCIFERASE; MANGANESE; PLASMID VECTOR; BACTERIAL DNA; ESCHERICHIA COLI PROTEIN; RECOMBINANT PROTEIN;

ARTICLE; CELL LYSATE; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; ENZYME ASSAY; ERROR; ESCHERICHIA COLI; EUKARYOTE; GENE ACTIVITY; GENE MUTATION; HUMAN; HUMAN CELL; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION; BIOSYNTHESIS; EVALUATION; GENETIC ENHANCEMENT; GENETICS; HELA CELL; METABOLISM; METHODOLOGY; MOLECULAR CLONING; MOLECULAR EVOLUTION; PROTEIN ENGINEERING; SITE DIRECTED MUTAGENESIS;

ESCHERICHIA COLI; EUKARYOTA; PROKARYOTA;

CLONING, MOLECULAR; DNA, BACTERIAL; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; EVOLUTION, MOLECULAR; GENETIC ENHANCEMENT; HELA CELLS; HUMANS; MUTAGENESIS, SITE-DIRECTED; POLYMERASE CHAIN REACTION; PROMOTER REGIONS (GENETICS); PROTEIN ENGINEERING; RECOMBINANT PROTEINS;

EID: 18944386294     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2004.08.015     Document Type: Article

References (17)

1. J. Abelson Directed evolution of nucleic acids by independent replication and selection Science 249 1990 488 489
2. F.H. Arnold Enzyme engineering reaches the boiling point Proc. Natl. Acad. Sci. USA 95 1998 2035 2036
3. S. Busby, and R.H. Ebright Promoter structure, promoter recognition, and transcription activation in prokaryotes Cell 79 1994 743 746
4. A. Crameri, S.A. Raillard, E. Bermudez, and W.P. Stemmer DNA shuffling of a family of genes from diverse species accelerates directed evolution Nature 391 1998 288 291
5. A. Crameri, E.A. Whitehorn, E. Tate, and W.P. Stemmer Improved green fluorescent protein by molecular evolution using DNA shuffling Nature Biotechnol. 14 1996 315 319
6. S.T. Estrem, T. Gaal, W. Ross, and R.L. Gourse Identification of an UP element consensus sequence for bacterial promoters Proc. Natl. Acad. Sci. USA 95 1998 9761 9766
7. L. Giver, and F.H. Arnold Combinatorial protein design by in vitro recombination Curr. Opin. Chem. Biol. 2 1998 335 338
8. L. Giver, A. Gershenson, P.O. Freskgard, and F.H. Arnold Directed evolution of a thermostable esterase Proc. Natl. Acad. Sci. USA 95 1998 12809 12813
9. S.H. Ke, and E.L. Madison Rapid and efficient site-directed mutagenesis by single-tube 'megaprimer' PCR method Nucleic Acids Res. 25 1997 3371 3372
10. M. Kohno, M. Enatsu, J. Funatsu, M. Yoshiizumi, and W. Kugimiya Improvement of the optimum temperature of lipase activity for Rhizopus niveus by random mutagenesis and its structural interpretation J. Biotechnol. 87 2001 203 210
11. J.C. Moore, and F.H. Arnold Directed evolution of a para-nitrobenzyl esterase for aqueous-organic solvents Nature Biotechnol. 14 1996 458 467
12. Z. Shao, and F.H. Arnold Engineering new functions and altering existing functions Curr. Opin. Struct. Biol. 6 1996 513 518
13. B. Van den Burg, G. Vriend, O.R. Veltman, G. Venema, and V.G. Eijsink Engineering an enzyme to resist boiling Proc. Natl. Acad. Sci. USA 95 1998 2056 2060
14. J.P. Vartanian, M. Henry, and S. Wain-Hobson Hypermutagenic PCR involving all four transitions and a sizeable proportion of transversions Nucleic Acids Res. 24 1996 2627 2631
15. C.R. Wagner, and S.J. Benkovic Site directed mutagenesis: a tool for enzyme mechanism dissection Trends Biotechnol. 8 1990 263 270
16. T. Yano, and H. Kagamiyama Directed evolution of ampicillin-resistant activity from a functionally unrelated DNA fragment: a laboratory model of molecular evolution Proc. Natl. Acad. Sci. USA 98 2001 903 907
17. H. Zhao, and F.H. Arnold Optimization of DNA shuffling for high fidelity recombination Nucleic Acids Res. 25 1997 1307 1308