Role of the pfcrt codon 76 mutation as a molecular marker for population-based surveillance of chloroquine (CQ)-resistant Plasmodium falciparum malaria in Ugandan sentinel sites with high CQ resistance

Transactions of the Royal Society of Tropical Medicine and Hygiene

Volumn 96, Issue 5, 2002, Pages 551-556

  Talisuna, Ambrose O ^g   Kyosiimire Lugemwa, Jackie ^a   Langi, Peter ^a   Mutabingwa, Theonest K ^b,c   Watkins, Williams ^d   Van Marck, Eric ^e   Egwang, Thomas ^b   D'Alessandro, Umberto ^f  

^a Med Biotech Laboratories Uganda   (Uganda)

^b NATIONAL INSTITUTE FOR MEDICAL RESEARCH   (Tanzania)

^c LONDON SCHOOL OF HYGIENE AND TROPICAL MEDICINE   (United Kingdom)

^d KEMRI WELLCOME TRUST RESEARCH PROGRAMME   (Kenya)

^e UNIVERSITY OF ANTWERP   (Belgium)

^f INSTITUTE OF TROPICAL MEDICINE   (Belgium)

^g NONE

Author keywords

Chloroquine; Drug resistance; Gene mutation; Genes; Malaria; Pfcrt; Plasmodium falciparum; Uganda

Indexed keywords

CARRIER PROTEIN; CHLOROQUINE; MOLECULAR MARKER;

ADOLESCENT; ADULT; AGE; ANTIBIOTIC RESISTANCE; ARTICLE; CHILD; CODON; COMMUNITY; CONTROLLED STUDY; CORRELATION ANALYSIS; DISEASE CARRIER; DRUG EFFICACY; DRUG USE; GENE MUTATION; GENOTYPE; GEOGRAPHIC DISTRIBUTION; HEALTH SURVEY; HUMAN; IN VIVO STUDY; MAJOR CLINICAL STUDY; MALARIA FALCIPARUM; NONHUMAN; PLASMODIUM FALCIPARUM; POPULATION RESEARCH; PREVALENCE; TIME; TREATMENT FAILURE; UGANDA; VALIDATION PROCESS; WILD TYPE;

PLASMODIUM FALCIPARUM; PROTOZOA;

EID: 18744368438     PISSN: 00359203     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0035-9203(02)90439-6     Document Type: Article

References (24)

1. Babiker, H. A., Pringle, S. J., Abdel-Muhsin, A., Mackinnon, M., Hunt, P. & Walliker, D. (2001). High level chloroquine resistance in Sudanese isolates of Plasmodium falciparum is associated with mutations in the chloroquine resistance transporter gene pfcrt and the multidrug resistance gene pfmdr1. Journal of Infectious Diseases, 183, 1535-1538.
2. Djimdé, A., Doumbo, O., Kanyetao, K., Diourte, Y., Coulibaly, D., Cortese, J., Su, X., Fidock, D., Nomura, T. & Plowe, C. (2001a). A molecular marker for CQ resistant falciparum malaria. New England Journal of Medicine, 344, 257-302.
3. Djimdé, A., Doumbo, O., Steketee, R. W. & Plowe C. V. (2001b). Application of a molecular marker for surveillance of chloroquine-resistant falciparum malaria. Lancet, 358, 890-891.
4. Dorsey, G., Kamya, M. R., Singh, A. & Rosenthal, P. J. (2001). Polymorphisms in the Plasmodium falciparum pfcrt and pfmdr1 genes and clinical response to CQ in Kampala, Uganda. Journal of Infectious Diseases, 183, 1417-1420.
5. Eggelte, T. A. (1990). Production of monoclonal antibodies against antimalarial drugs for use in immuno assays. In: The Validation of Chemical and Immunological Tests for Antimalarials in Body Fluids, Navaratnam, V. & Payne, D. (editors). University Sains Malaysia, Penang: Centre for Drug Research International Monograph Series 3, pp. 35-63.
6. Eggelte, T. A., Sondij, S. & Gussenhove, G. (1992). Simple dipstick tests for the determination of antimalarials in body fluids. In: Proceedings of the XIIIth International Congress for Tropical Medicine and Malaria, Pattaya, Thailand, 29 November-4 December 1992, 2, 242.
7. Fidock, D. A., Nomura, T., Talley, A. K., Cooper, R. A., Dzekunov, S. M., Ferdig, M. T., Ursos, L. M. B., Sidhu, A. S., Naude, B., Deitsch, K. W., Su, X.-Z., Wootton, J. C., Roepe, P. D. & Wellems, T. E. (2000). Mutations in the P. falciparum digestive vacuole transmembrane protein PfCRT and evidence for their role in chloroquine resistance. Molecular Cell, 6, 861-871.
8. Fogh, S., Jepsen, S. & Efferse, P. (1979). Chloroquine-resistant Plasmodium falciparum malaria in Kenya. Transactions of the Royal Society of Tropical Medicine and Hygiene, 73, 228-229.
9. Foote, S. J., Kyle, D. E., Martin, R. K., Oduola, A. M., Forsyth, K., Kemp, D. J. & Cowman, A. F. (1990). Several genotypes of the multidrug-resistance gene are closely linked to chloroquine resistance in Plasmodium falciparum. Nature, 345, 255-258.
10. Foster, S. (1995). Treatment of malaria outside the formal health services. Journal of Tropical Medicine and Hygiene, 98, 29-34.
11. Hastings, I. M. & D'Alessandro, U. (2000). Modelling a predictable disaster: the rise and spread of drug-resistant malaria. Parasitology Today, 16, 340-347.
12. Kitua, A. Y. (2000). Antimalarial drug policy: making systematic change. Lancet, 354, supplement SIV, 32.
13. Kyosiimire-Lugemwa, J., Nalunkuma-Kazibwe, A. J., Mujuzi, G., Mulindwa, H., Talisuna, A. & Egwang, T. G. (2002). The Lys76-Thr mutation in PfCRT and chloroquine resistance in Plasmodium falciparum isolates from Uganda. Transactions of the Royal Society of Tropical Medicine and Hygiene, 96, 91-95.
14. Lemeshow, S. & Taber, S. (1991). Lot quality assurance sampling: single and double sampling plans. World Health Statistics Quarterly, 44, 115-132.
15. Mantel, N. & Haenszel, W. (1959). Statistical aspects of the analysis of data from retrospective studies of disease. Journal of the National Cancer Institute, 22, 719-748.
16. McCombie, S. C. (1996). Treatment seeking for malaria, a review of recent research. Social Science and Medicine, 43, 933-945.
17. Metselaar, D. & Thiel, P. H. (1959). Classification of malaria. Tropical and Geographical Medicine, 11, 157-161.
18. Plowe, C. V., Djimde, A., Bouare, M., Doumbo, O. & Wellems, T. E. (1995). Pyrimethamine and proguanil resistance conferring mutations in Plasmodium falciparum dihydrofolate reductase: polymerase chain reaction methods for surveillance in Africa. American Journal of Tropical Medicine and Hygiene, 52, 565-568.
19. Plowe, C. V., Cortese, J. F., Djimde, A., Nwanyanwu, O. C., Watkins, W. M., Winstanley, P. A., Estrada-Franco, J. G., Mollinedo, R. E., Avila, J. C., Cespedes, J. L., Carter, D. & Doumbo, O. K. (1997). Mutations in Plasmodium falciparum dihydrofolate reductase and dihydropteroate synthase and epidemiological patterns of pyrimethamine-sulfadoxine use and resistance. Journal of Infectious Diseases, 176, 1590-1596.
20. Schwick, P., Eggelte, T. A., Hess, F., Tueumuna, T. T., Payne, D., Nothdurft, H. D., von Sonnenburg, F. & Löscher, T. (1998). Sensitive ELISA dipstick test for the detection of chloroquine in urine under field conditions. Tropical Medicine and International Health, 3, 828-832.
21. Su, X., Kirkman, L. A., Fujioka, H. & Wellems, T. E. (1997). Complex polymorphisms in an approximately 330kDa protein are linked to chloroquine-resistant P. falciparum in Southeast Asia and Africa. Cell, 91, 593-603.
22. Takechi, M., Matuso, M., Ziba, C., Macheso, A., Butao, D., Zungu, I. L., Chakanika, I. & Bustos, Ma. D. G. (2001). Therapeutic efficacy of sulphadoxine/pyrimethamine and susceptibility in vitro of P. falciparum isolates to sulphadoxine-pyrimethamine and other antimalarial. drugs in Malawian children. Tropical Medicine and International Health, 6, 429-434.
23. Wernsdorfer, W. H. (1991). The development and spread of drug-resistant malaria. Parasitology Today, 11, 297-303.
24. WHO (1996). Assessment of therapeutic efficacy of antimalarial drugs for uncomplicated falciparum malaria in areas with intense transmission. Geneva: World Health Organization. WHO/MAL/96.1077.