HLA-DR binding prediction and experimental evaluation of T-cell epitopes of mycolyl transferase 85B (Ag85B), a major secreted antigen of Mycobacterium tuberculosis

Medical Principles and Practice

Volumn 14, Issue 3, 2005, Pages 140-146

  Mustafa, Abu S ^a   Abal, Adnan T ^a,b   Shaban, Fatma ^a   El Shamy, Abdulsalam M ^b   Amoudy, Hanady A ^a  

^a KUWAIT UNIVERSITY   (Kuwait)

^b Chest Diseases Hospital Kuwait   (Kuwait)

Author keywords

Ag85b; HLA DR binding prediction; Mycobacterium tuberculosis; T cell epitopes

Indexed keywords

EPITOPE; HLA DR ANTIGEN; MYCOBACTERIUM ANTIGEN; MYCOLYL TRANSFERASE 85B; UNCLASSIFIED DRUG;

ALLELE; AMINO ACID SEQUENCE; ANTIGEN BINDING; ANTIGEN SPECIFICITY; ARTICLE; CLINICAL ARTICLE; CONTROLLED STUDY; HUMAN; HUMAN CELL; LYMPHOCYTE PROLIFERATION; MOLECULAR RECOGNITION; MYCOBACTERIUM TUBERCULOSIS; PERIPHERAL BLOOD MONONUCLEAR CELL; PREDICTION; T LYMPHOCYTE;

ACYLTRANSFERASES; ALLELES; ANTIGENS, BACTERIAL; BACTERIAL PROTEINS; EPITOPES, T-LYMPHOCYTE; HLA-DR ANTIGENS; HUMANS; MYCOBACTERIUM TUBERCULOSIS; T-LYMPHOCYTES;

EID: 18644371000     PISSN: 10117571     EISSN: None     Source Type: Journal    
DOI: 10.1159/000084629     Document Type: Article

References (37)

1. Frieden TR, Sterling TR, Munsiff SS, Watt CJ, Dye C: Tuberculosis. Lancet 2003;362:887-899.
2. Behbehani N, Abal A, Al-Shami A, Enarson, DA: Epidemiology of tuberculosis in Kuwait from 1965 to 1999. Int J Tuberc Lung Dis 2002;6:465-469.
3. Mustafa AS: Biotechnology in the development of new vaccines and diagnostic reagents against tuberculosis. Curr Pharm Biotechnol 2001;2:157-173.
4. Mustafa AS: Development of new vaccines and diagnostic reagents against tuberculosis. Mol Immunol 2002;39:113-119.
5. Mustafa AS, Al-Attiyah R: Looking beyond BCG vaccines. J Postgrad Med 2003;49:143-140.
6. Ahmad S, Amoudy HA, Thole JE, Young DB, Mustafa AS: Identification of a novel protein antigen encoded by a Mycobacterium tuberculosis-specific RD1 region gene. Scand J Immunol 1999;49:515-522.
7. Ahmad S, Ali MM, Mustafa AS: Construction of a modified vector for efficient purification of recombinant Mycobacterium tuberculosis proteins expressed in Escherichia coli. Protein Expr Purif 2003;29:167-175.
8. Mustafa AS, Lundin KE, Meloen RH, Shinnick TM, Oftung F: Identification of promiscuous epitopes from the mycobacterial 65-kilodalton heat shock protein recognized by human CD4(+) T cells of the Mycobacterium leprae memory repertoire. Infect Immun 1999;67: 5683-5689.
9. Mustafa AS: Identification of T-cell-activating recombinant antigens shared among three candidate antileprosy vaccines, killed M. leprae, M. bovis BCG, and Mycobacterium w. Int J Lepr Other Mycobact Dis 1988;56:265-273.
10. Haanen JB, Ottenhoff TH, Lai A, Fat RF, Soebono H, Spits H, de Vries RR: Mycobacterium leprae-specific T cells from a tuberculoid leprosy patient suppress HLA-DR3-restricted T cell responses to an immunodominant epitope on 65-kDa hsp of mycobacteria. J Immunol 1990;145:3898-3904.
11. Oftung F, Lundin KEA, Geluk A, Shinnick TM, Meloen R, Mustafa AS: Primary structure and MHC restriction of peptide defined T cell epitopes from recombinantly expressed mycobacterial protein antigens. Med Princ Pract 1997;6:66-73.
12. Ravn P, Demissie A, Eguale T, Wondwosson H, Lein D, Amoudy HA, Mustafa AS, Jensen AK, Holm A, Rosenkrands I, Oftung F, Olobo J, von Reyn F, Andersen P: Human T cell responses to the ESAT-6 antigen from Mycobacterium tuberculosis. J Infect Dis 1999;179: 637-645.
13. Arend SM, van Meijgaarden KE, de Boer K, de Palou EC, van Soolingen D, Ottenhoff TH, van Dissel JT: Tuberculin skin testing and in vitro T cell responses to ESAT-6 and culture filtrate protein 10 after infection with Mycobacterium marinum or M. kansasii. J Infect Dis 2002;186:1797-1807.
14. Arend SM, Geluk A, van Meijgaarden KE, van Dissel JT, Theisen M, Andersen P, Ottenhoff TH: Antigenic equivalence of human T-cell responses to Mycobacterium tuberculosis-specific RD1-encoded protein antigens ESAT-6 and culture filtrate protein 10 and to mixtures of synthetic peptides. Infect Immun 2000;68: 3314-3321.
15. Mustafa AS, Cockle PJ, Shaban F, Hewinson RG, Vordermeier HM: Immunogenicity of Mycobacterium tuberculosis RD1 region gene products in infected cattle. Clin Exp Immunol 2002;130:37-42.
16. Vordermeier HM, Whelan A, Cockle PJ, Farrant L, Palmer N, Hewinson RG: Use of synthetic peptides derived from the antigens ESAT-6 and CFP-10 for differential diagnosis of bovine tuberculosis in cattle. Clin Diagn Lab Immunol 2001;8:571-578.
17. Olsen AW, Hansen PR, Holm A, Andersen P: Efficient protection against Mycobacterium tuberculosis by vaccination with a single sub-dominant epitope from the ESAT-6 antigen. Eur J Immunol 2000;30:1724-1732.
18. Mustafa AS, Shaban FA, Abal AT, Al-Attiyah R, Wiker HG, Lundin KE, Oftung F, Huygen K: Identification and HLA restriction of naturally derived Th1-cell epitopes from the secreted Mycobacterium tuberculosis antigen 85B recognized by antigen-specific human CD4(+) T-cell lines. Infect Immun 2000;68:3933-3940.
19. Al-Attiyah R, Shaban FA, Wiker HG, Oftung F, Mustafa AS: Synthetic peptides identify promiscuous human Th1 cell epitopes of the secreted mycobacterial antigen MPB70. Infect Immun 2003;71:1953-1960.
20. Al-Attiyah R, Mustafa AS: Computer-assisted prediction of HLA-DR binding and experimental analysis for human promiscuous Th1 cell epitopes in a novel 24-kDa secreted lipoprotein (LppX) of Mycobacterium tuberculosis. Scand J Immunol 2004;59:16-24.
21. Panigada M, Sturniolo T, Besozzi G, Boccieri MG, Sinigaglia F, Grassi GG, Grassi F: Identification of a promiscuous T-cell epitope in Mycobacterium tuberculosis Mce proteins. Infect Immun 2002;70:79-85.
22. Feller DC, de la Cruz VF: Identifying antigenic T cell sites. Nature 1991;349:720-721.
23. De Groot AS, Jesdale B, Szu E, Schafer JR, Chics RM, Deocampo G: An interactive Web site providing major histocompatibility ligand predictions: Application to HIV research. AIDS Res Hum Retroviruses 1997;13:529-531.
24. Hammer J, Bono E, Gallazzi F, Belunis C, Nagy Z, Sinigaglia F: Precise prediction of major histocompatibility complex class-II peptide interaction based on peptide side chain scanning. J Exp Med 1994;180:2353-2358.
25. Sturniolo T, Bono E, Ding J, Raddrizzani L, Tuereci O, Sahin M, Braxenthaler F, Gazzali F, Protti MP, Sinigaglia F, Hammer J: Generation of tissue-specific and promiscuous HLA ligand databases using DNA microarrays and virtual HLA class II matrices. Nat Biotechnol 1999;17:555-561.
26. Singh H, Raghava GPS: ProPred: Prediction of HLA-DR binding sites. Bioinformatics 2001;17:1236-1237.
27. Mustafa AS, Amoudy HA, Wiker HG, Abal AT, Ravn P, Oftung F, Andersen P: Comparison of antigen-specific T-cell responses of tuberculosis patients using complex or single antigens of Mycobacterium tuberculosis. Scand J Immunol 1998;48:535-543.
28. Mustafa AS, Shaban FA, Al-Attiyah R, Abal AT, El-Shamy AM, Andersen P, Oftung F: Human Th1 cell lines recognize the Mycobacterium tuberculosis ESAT-6 antigen and its peptides in association with frequently expressed HLA class II molecules. Scand J Immunol 2003;57:125-134.
29. Al-Attiyah R, Mustafa AS, Abal AT, Madi NM, Andersen P: Restoration of mycobacterial antigen-induced proliferation and interferon-gamma responses in peripheral blood mononuclear cells of tuberculosis patients upon effective chemotherapy. FEMS Immunol Med Microbiol 2003;38:249-256.
30. Mustafa AS, Oftung F, Amoudy HA, Madi NM, Abal AT, Shaban F, Rosen Krands I, Andersen P: Multiple epitopes from the Mycobacterium tuberculosis ESAT-6 antigen are recognized by antigen-specific human T cell lines. Clin Infect Dis 2000;30(suppl 3):S201-S205.
31. Mustafa AS, Lundin KE, Oftung F: Isolation of recombinant phage clones expressing mycobacterial T cell antigens by screening a recombinant DNA library with human CD4+ Th1 clones. FEMS Immunol Med Microbiol 1998; 22:205-216.
32. Tuberculist Web Server, http://genolist.pasteur.fr/TubercuList.
33. Silver RF, Wallis RS, Ellner JJ: Mapping of T cell epitopes of the 30-kDa alpha antigen of Mycobacterium bovis strain bacillus Calmette-Guérin in purified protein derivative (PPD)-positive individuals. J Immunol 1995;154: 4665-4674.
34. Goldsby RA, Kindt TJ, Osborne BA: Kuby Immunology, ed 4. New York, Freeman, 2000, p 186.
35. Hammer J, Sturniolo T, Sinigaglia F: HLA class II peptide binding specificity and autoimmunity. Adv Immunol 1997;66:67-100.
36. Manici S, Sturniolo T, Imro MA, Hammer J, Sinigaglia F, Noppen C, Spagnoli G, Mazzi B, Bellone M, Dellabona P, Protti MP: Melanoma cells present a MAGE-3 epitope to CD4+ cytotoxic T cells in association with histocompatibility leukocyte antigen DR11. J Exp Med 1999;189:871-876.
37. Vordermeier M, Whelan AO, Hewinson RG: Recognition of mycobacterial epitopes by T cells across mammalian species and use of a program that predicts human HLA-DR binding peptides to predict bovine epitopes. Infect Immun 2003;71:1980-1987.