Microscale synthesis of isotopically labeled R-[6-xH]N 5,N10-methylene-5,6,7,8-tetrahydrofolate as a cofactor for thymidylate synthase

Analytical Biochemistry

Volumn 328, Issue 1, 2004, Pages 44-50

  Agrawal, Nitish ^a   Mihai, Cornelia ^a   Kohen, Amnon ^a  

^a University of Iowa   (United States)

Author keywords

Dihydrofolate reductase; Kinetic isotope effect; Labeled folate; Methylene tetrahydrofolate; Thermoanaerobium brockii alcohol dehydrogenase; Thymidylate synthase; Thymine

Indexed keywords

ENZYMES; ESCHERICHIA COLI; NUCLEOTIDES; RATE CONSTANTS; TRITIUM;

ALCOHOL DEHYDROGENASE; DIHYDROFOLATE REDUCTASE; KINETIC ISOTOPE EFFECTS; LABELED FOLATE; TETRAHYDROFOLATES; THYMIDYLATE SYNTHASE; THYMINE;

HIGH PRESSURE LIQUID CHROMATOGRAPHY;

7,8 DIHYDROFOLIC ACID; DEUTERIUM; DIHYDROFOLIC ACID; FOLIC ACID DERIVATIVE; FORMALDEHYDE; HYDROGEN; N5,N10 METHYLENE 5,6,7,8 TETRAHYDROFOLIC ACID; NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; TETRAHYDROFOLIC ACID; TETRAHYDROFOLIC ACID DERIVATIVE; THYMIDYLATE SYNTHASE; TRITIUM; UNCLASSIFIED DRUG;

ARTICLE; CATALYSIS; CATALYST; CONTROLLED STUDY; ENZYME MECHANISM; ENZYME PURIFICATION; ENZYME SYNTHESIS; ESCHERICHIA COLI; FREEZE DRYING; ISOTOPE LABELING; NONHUMAN; OXIDATION; PRIORITY JOURNAL; RACEMIC MIXTURE; REDUCTION; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; STEREOSPECIFICITY;

ESCHERICHIA COLI; THERMOANAEROBACTER; THERMOANAEROBACTER BROCKII;

EID: 1842736541     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ab.2004.01.029     Document Type: Article

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