Luciferase-based transgenic recombination assay is more sensitive than β-glucoronidase-based

Mutation Research - Genetic Toxicology and Environmental Mutagenesis

Volumn 559, Issue 1-2, 2004, Pages 189-197

  Ilnytskyy, Yaroslav ^a   Boyko, Alexander ^a   Kovalchuk, Igor ^a  

^a UNIVERSITY OF LETHBRIDGE   (Canada)

Author keywords

Glucoronidase; Luciferase; Recombination assay

Indexed keywords

BETA GLUCURONIDASE; LUCIFERASE;

ACCURACY; ANALYTIC METHOD; ARABIDOPSIS; ARTICLE; ASSAY; CONTROLLED STUDY; GENE ACTIVITY; GENE EXPRESSION; HOMOLOGOUS RECOMBINATION; INTERMETHOD COMPARISON; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; RECOMBINANT DNA TECHNOLOGY; RECOMBINATION ASSAY; TOBACCO; TRANSGENE; TRANSGENIC PLANT;

ARABIDOPSIS; DNA PRIMERS; DNA REPAIR; GENE EXPRESSION; GENETIC MARKERS; GENETIC TECHNIQUES; GENOME, PLANT; GENOMIC INSTABILITY; GLUCURONIDASE; HISTOLOGICAL TECHNIQUES; LUCIFERASES; PLANTS, GENETICALLY MODIFIED; RECOMBINATION, GENETIC; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; TOBACCO; TRANSGENES;

ARABIDOPSIS; NICOTIANA OBTUSIFOLIA; NICOTIANA TABACUM;

EID: 1842527032     PISSN: 13835718     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mrgentox.2004.02.001     Document Type: Article

References (49)

1. Karanjawala Z.E., Murphy N., Hinton D.R., Hsieh C.L., Lieber M.R. Oxygen metabolism causes chromosome breaks and is associated with the neuronal apoptosis observed in DNA double-strand break repair mutants. Curr. Biol. 12:2002;397-402.
2. Rinaldo C., Bazzicalupo P., Ederle S., Hilliard M., La Volpe A. Role of Caenorhabditis elegans rad-51 in meiosis and in resistance to ionizing radiation during development. Genetics. 160:2002;471-479.
3. Sargent R., Brenneman M., Wilson J. Repair of site-specific double-strand breaks in a mammalian chromosome by homologous and illegitimate recombination. Mol. Cell Biol. 17(2):1997;67-277.
4. Liang F., Han M., Romanienko P., Jasin andM. Homology-directed repair is major double-strand break repair pathway in mammalian cells. Proc. Natl. Acad. Sci. U.S.A. 95:1998;5172-5177.
5. Critchlow S., Jackson S. DNA end-joining: from yeast to man. TIBS. 23:1998;394-402.
6. Roth D., Wilson J. Nonhomologous recombination in mammalian cells: role for short sequence homologies in the joining reaction. Mol. Cell. Biol. 6:1986;4295-4304.
7. Pfeiffer P. The mutagenic potential of DNA double-strand break repair. Toxicol. Lett. 96-97:1998;119-129.
8. Hagmann M., Adlkofer K., Pfeiffer P., Bruggmann R., Georgiev O., Rungger D., Schaffner W. Dramatic changes in the ratio of homologous recombination to nonhomologous DNA-end joining in oocytes and early embryos of Xenopus laevis. Biol. Chem. Hoppe Seyler. 377:1996;239-250.
9. Essers J., van Steeg H., de Wit J., Swagemakers S., Vermeij M., Hoeijmakers J., Kanaar R. Homologous and nonhomologous recombination differentially affect DNA damage repair in mice. EMBO J. 19:2000;1703-1710.
10. Kovalchuk I., Kovalchuk O., Arkhipov A., Hohn B. Transgenic plants are sensitive bioindicators of nuclear pollution caused by the Chernobyl accident. Nat. Biotechnol. 16:1998;1054-1059.
11. Puchta H., Swoboda P., Hohn B. Induction of homologous DNA recombination in whole plants. Plant J. 7:1995;203-210.
12. Ries G., Heller W., Puchta H., Sandermann H., Seidlitz H.K., Hohn B. Elevated UV-B radiation reduces genome stability in plants. Nature. 406:2000;98-101.
13. Kovalchuk O., Titov V., Hohn B., Kovalchuk I. A sensitive transgenic plant system to detect toxic inorganic compounds in the environment. Nat. Biotechnol. 19:2001;568-572.
14. Ries G., Buchholz G., Frohnmeyer H., Hohn B. UV-damage-mediated induction of homologous recombination in Arabidopsis is dependent on photosynthetically active radiation. Proc. Nat. Acad. Sci. U.S.A. 97:2000;13425-13429.
15. Kovalchuk I., Filkowski J., Smith K., Kovalchuk O. The dualistic nature of radicals: the high-low phenomenon. Plant Cell Environ. 26:2003;1531-1539.
16. Kovalchuk I., Kovalchuk O., Kalck V., Boyko V., Filkowski J., Heinlein M., Hohn B. Pathogen induced systemic plant signal triggers DNA rearrangements. Nature. 423:2003;760-762.
17. Britt A.B. Repair of DNA damage induced by ultraviolet radiation. Plant Physiol. 108:1995;891-896.
18. Tuteja N., Singh M.B., Misra M.K., Bhalla P.L., Tuteja R. Molecular mechanisms of DNA damage and repair: progress in plants. Crit. Rev. Biochem. Mol. Biol. 36:2001;337-397.
19. Hollosy F. Effects of ultraviolet radiation on plant cells. Micron. 33:2002;179-197.
20. Li A., Schurmann D., Gallego F., Kovalchuk I., Tinland B. Repair of damaged DNA by Arabidopsis cell extract. Plant Cell. 14:2002;263-273.
21. Kirik A., Salomon S., Puchta H. Species-specific double-strand break repair and genome evolution in plants. EMBO J. 19:2000;5562-5566.
22. Gorbunova V., Avivi-Ragolski N., Shalev G., Kovalchuk I., Abbo S., Hohn B., Levy A. A new hyperrecombinogenic mutant of Nicotiana tabacum. Plant J. 24:2000;601-611.
23. Pelczar P., Kalk V., Kovalchuk I. Human and tobacco: organisms with similar genome size but different genome maintenance strategy. J. Mol. Biol. 331:2003;771-779.
24. I. Kovalchuk, P. Pelczar, O. Kovalchuk, The high frequency of nucleotide misincorporations upon the processing of the double-strand breaks, DNA Repair 3 (2004) 217-223.
25. Rasmuson A. Mutagenic effects of some water-soluble metal compounds in a somatic eye-color test system in Drosophila melanogaster. Mut. Res. 157:1985;157-162.
26. Sacco M.G.et al. A transgenic mouse model for the detection of cellular stress induced by toxic inorganic compounds. Nat. Biotechnol. 15:1997;1392-1397.
27. Mayer C., Klein R.G., Wesch H., Schmezer P. Nickel subsulfide is genotoxic in vitro but shows no mutagenic potential in respiratory tract tissues of BigBlue rats and Muta Mouse mice in vivo after inhalation. Mut. Res. 420:1998;85-98.
28. Hendricks C.A., Almeida K.H., Stitt M.S., Jonnalagadda V.S., Rugo R.E., Kerrison G.F., Engelward B.P. Spontaneous mitotic homologous recombination at an enhanced yellow fluorescent protein (EYFP) cDNA direct repeat in transgenic mice. Proc. Natl. Acad. Sci. U.S.A. 100:2003;6325-6330.
29. Amanuma K., Takeda H., Amanuma H., Aoki Y. Transgenic zebrafish for detecting mutations caused by compounds in aquatic environments. Nat. Biotechnol. 18:2000;62-65.
30. Fiskesjo G. The Allium test-an alternative in environmental studies: the relative toxicity of metal ions. Mut. Res. 197:1988;243-260.
31. Kovalchuk O., Kovalchuk I., Arkhipov A., Telyuk P., Hohn B., Kovalchuk L. The Allium cepa chromosome aberration test reliably measures genotoxicity of soils of inhabited areas in the Ukraine contaminated by the Chernobyl accident. Mut. Res. 415:1998;47-57.
32. Kanaya N., Gill B., Grover I., Murin A., Osiecka R., Sandhu S., Andersson H. Vicia faba chromosomal aberration assay. Mut. Res. 310:1994;231-247.
33. Ichikawa S. Tradescantia stamen-hair system as an excellent botanical tester of mutagenicity: its responses to ionizing radiations and chemical mutagens, and some synergistic effects found. Mut. Res. 270:1992;3-22.
34. Tice R.R., Agurell E., Anderson D., Burlinson B., Hartmann A., Kobayashi H., Miyamae Y., Rojas E., Ryu J.C., Sasaki Y.F. Single cell gel/comet assay: guidelines for in vitro and in vivo genetic toxicology testing. Environ. Mol. Mutagen. 35:2000;206-221.
35. Kovalchuk I., Kovalchuk O., Hohn B. Genome-wide variation of the somatic mutation frequency in transgenic plants. EMBO J. 19:2000;4431-4438.
36. J. Besplug, J. Filkowski, P. Burke, I. Kovalchuk, O. Kovalchuk, Atrazine induces homologous recombination but not point mutation in the transgenic plant-based biomonotoring assay, Arch. Environ. Contamin. Toxicol., in press.
37. J. Filkowski, J. Besplug, P. Burke, I. Kovalchuk, O. Kovalchuk, Recombination- and point mutation-monitoring transgenic plants reveal the genotoxicity of commonly used herbicides 2,4-D and dicamba, Mut. Res. 542 (2003) 23-32.
38. Puchta H., Swoboda P., Gal S., Blot M., Hohn B. Somatic intrachromosomal homologous recombination events in populations of plant siblings. Plant Mol. Biol. 28:1995;281-292.
39. Masson J.E., Paszkowski J. Arabidopsis thaliana mutants altered in homologous recombination. Proc. Natl. Acad. Sci. U.S.A. 94:1997;11731-11735.
40. Mengiste T., Revenkova E., Bechtold N., Paszkowski J. An SMC-like protein is required for efficient homologous recombination in Arabidopsis. EMBO J. 18:1999;4505-4512.
41. Hanin M., Mengiste T., Bogucki A., Paszkokwski J. Elevated levels of intrachromosomal homologous recombination in Arabidopsis overexpressing the MIM gene. Plant J. 24:2000;183-189.
42. Gherbi H., Gallego M., Jalut N., Lucht J., Hohn B., White C. Homologous recombination in planta is stimulated in the absence of Rad50as. EMBO Rep. 2:2001;287-291.
43. Lebel E.G., Masson J., Bogucki A., Paszkowski J. Stress-induced intrachromosomal recombination in plant somatic cells. Proc. Natl. Acad. Sci. U.S.A. 90:1993;422-426.
44. Swoboda P., Hohn B., Gal S. Somatic homologous recombination in planta: the recombination frequency is dependent on the allelic state of recombining sequences and may be influenced by genomic position effects. Mol. Gen. Genet. 237:1993;33-40.
45. Swoboda P., Gal S., Hohn B., Puchta H. Intrachromosomal homologous recombination in whole plants. EMBO J. 13:1994;484-489.
46. J. Filkowski, O. Kovalchuk, I. Kovalchuk, Dissimilar mutation and recombination rates in Arabidopsis and tobacco, Plant Sci. 166 (2004) 265-272.
47. Jefferson R. Assaying chimeric genes in plants: the GUS gene fusion system. Plant Mol. Biol. Rep. 5:1987;387-405.
48. Benfey P., Ren L., Chua N. Tissue-specific expression from CaMV 35S enhancer subdomains in early stages of plant development. EMBO J. 9:1990;1677-1684.
49. Hanin M., Paszkowski J. Plant genome modification by homologous recombination. Curr. Opin. Plant Biol. 6:2003;157-162.