Recombinant murine-activated protein C is neuroprotective in a murine ischemic stroke model

Blood Cells, Molecules, and Diseases

Volumn 30, Issue 3, 2003, Pages 271-276

  Fernández, José A ^a   Xu, Xiao ^a   Liu, Dong ^b   Zlokovic, Berislav V ^b   Griffin, John H ^a  

^a SCRIPPS RESEARCH INSTITUTE   (United States)

^b UNIVERSITY OF ROCHESTER MEDICAL CENTER   (United States)

Author keywords

Ischemia; Mouse; Protein C; Stroke; Thrombosis

Indexed keywords

ACTIVATED PROTEIN C; COMPLEMENTARY DNA; PROTEIN S; RECOMBINANT PROTEIN;

ANIMAL MODEL; ANTICOAGULATION; ARTICLE; BRAIN BLOOD FLOW; CEREBROVASCULAR ACCIDENT; CONTROLLED STUDY; DOSE RESPONSE; DRUG ACTIVITY; DRUG EFFICACY; HUMAN; HYDROLYSIS; IN VITRO STUDY; IN VIVO STUDY; MALE; MIDDLE CEREBRAL ARTERY OCCLUSION; MOUSE; NEUROPROTECTION; NONHUMAN; NUCLEOTIDE SEQUENCE; PARTIAL THROMBOPLASTIN TIME; PRIORITY JOURNAL; SPECIES DIFFERENCE;

MURINAE;

EID: 17944396396     PISSN: 10799796     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1079-9796(03)00034-2     Document Type: Article

References (25)

1. Esmon C.T., Fukudome K. Cellular regulation of the protein C pathway. Semin. Cell Biol. 6:1995;259-268.
2. Taylor F.B., Peer G.T., Lockhart M.S., Ferrell G., Esmon C.T. Endothelial cell protein C receptor plays an important role in protein C activation in vivo. Blood. 97:2001;1685-1688.
3. Walker F.J., Sexton P.W., Esmon C.T. The inhibition of blood coagulation by activated Protein C through the selective inactivation of activated Factor V. Biochim. Biophys. Acta. 571:1979;333-342.
4. Fulcher C.A., Gardiner J.E., Griffin J.H., Zimmerman T.S. Proteolytic inactivation of human factor VIII procoagulant protein by activated human protein C and its analogy with factor V. Blood. 63:1984;486-489.
5. Hanson S.R., Griffin J.H., Harker L.A., Kelly A.B., Esmon C.T., Gruber A. Antithrombotic effects of thrombin-induced activation of endogenous protein C in primates. J. Clin. Invest. 92:1993;2003-2012.
6. Griffin J.H., Zlokovic B., Fernandez J.A. Activated protein C potential therapy for severe sepsis, thrombosis, and stroke . Semin. Hematol. 39:2002;197-205.
7. Bernard G.R., et al. Efficacy and safety of recombinant human activated protein C for severe sepsis. N. Engl. J. Med. 344:2001;699-709.
8. Dahlbäck B., Hildebrand B., Malm J. Characterization of functionally important domains in human vitamin K-dependent protein S using monoclonal antibodies. J. Biol. Chem. 265:1990;8127-8135.
9. He X., Shen L., Dahlbäck B. Expression and functional characterization of chimeras between human and bovine vitamin-K-dependent protein-S-defining modules important for the species specificity of the activated protein C cofactor activity. Eur. J. Biochem. 227:1995;433-440.
10. Hackeng T.M., et al. Protein S binding to human endothelial cells is required for expression of cofactor activity for activated protein C. J. Biol. Chem. 268:1993;3993-4000.
11. Heeb M.J., Rosing J., Bakker H.M., Fernández J.A., Tans G., Griffin J.H. Protein S binds to and inhibits factor Xa. Proc. Natl. Acad. Sci. USA. 91:1994;2728-2732.
12. Tada N., Sato M., Tsujimura A., Iwase R., Hashimoto-Gotoh T. Isolation and characterization of a mouse protein C cDNA. J. Biochem.Tokyo. 111:1992;491-495.
13. Shibata M., et al. Anti-inflammatory, antithrombotic and neuroprotective effects of activated protein C in a murine model of focal ischemic stroke. Circulation. 103:2001;1799-1805.
14. Chase T., Shaw E. p-Nitrophenyl-p′-guanidinobenzoate HCl a new active site titrant for trypsin . Biochem. Biophys. Res. Commun. 29:1967;508-514.
15. Gale A.J., Heeb M.J., Griffin J.H. The autolysis loop of activated protein C interacts with factor Va and differentiates between the Arg506 and Arg306 cleavage sites. Blood. 96:2000;585-593.
16. Tabrizi P., et al. Tissue plasminogen activator (tPA)deficiency exacerbates cerebrovascular fibrin deposition and brain injury in a murine stroke model studies in tPA-deficient mice and wild-type mice on a matched genetic background . Arterioscler. Thromb. Vasc. Biol. 19:1999;2801-2806.
17. Jalbert L.R., Rosen E.D., Lissens A., Carmeliet P., Collen D., Castellino F.J. Nucleotide structure and characterization of the murine gene encoding anticoagulant protein C. Thromb. Haemost. 79:1998;310-316.
18. Foster D.C., Yoshitake S., Davie E.W. The nucleotide sequence of the gene for human protein C. Proc. Natl. Acad. Sci. USA. 82:1985;4673-4677.
19. Walker F.J. Regulation of bovine-activated protein C by protein S the role of the cofactor protein in species specificity . Thromb. Res. 22:1981;321-327.
20. Holly R.D., Foster D.C. Resistance to inhibition by alpha-1-anti-trypsin and species specificity of a chimeric human/bovine protein C. Biochemistry. 33:1994;1876-1880.
21. Shen L., He X., Dahlback B. Synergistic cofactor function of factor V and protein S to activated protein C in the inactivation of the factor VIIIa-factor IXa complex - species specific interactions of components of the protein C anticoagulant system. Thromb. Haemost. 78:1997;1030-1036.
22. Gruber A., et al. Inhibition of thrombus formation by activated recombinant protein C in a primate model of arterial thrombosis. Circulation. 82:1990;578-585.
23. Taylor F.B., Chang A., Esmon C.T., D'Angelo A., Vigano-D'Angelo S., Blick K.E. Protein C prevents the coagulopathic and lethal effects of Escherichia coli infusion in the baboon. J. Clin. Invest. 79:1987;918-925.
24. Jalbert L.R., et al. Inactivation of the gene for anticoagulant protein C causes lethal perinatal consumptive coagulopathy in mice. J. Clin. Invest. 102:1998;1481-1488.
25. T. Cheng, et al., Activated protein C blocks p53-mediated apoptosis in ischemic human brain endothelium and is neuroprotective, Nat. Med. 9 (2003) 338-342.