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BioTechniques
Volumn 27, Issue 3, 1999, Pages 444-445
Nucleic acid removal from Taq polymerase preparations using an aqueous/organic biphasic system
Author keywords

[No Author keywords available]
Indexed keywords

TAQ POLYMERASE;
EID: 0032827914     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/99273bm09     Document Type: Article
Times cited : (8)
References (8)
  • 1
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    • Deoxyribonucleic acid polymerase from the extreme thermophile Thermus aquaticus
    • Chien, A., D.B. Edgar and J.M. Trela. 1976. Deoxyribonucleic acid polymerase from the extreme thermophile Thermus aquaticus. J. Bacteriol. 127:1550-1557.
    • (1976) J. Bacteriol. , vol.127 , pp. 1550-1557
    • Chien, A.1    Edgar, D.B.2    Trela, J.M.3
  • 2
    • 0028827616 scopus 로고
    • Singlestep purification of a thermostable DNA polymerase expressed in Escherichia coli
    • Desai, U.J. and P.K. Pfaffle. 1995. Singlestep purification of a thermostable DNA polymerase expressed in Escherichia coli. BioTechniques 19:780-784.
    • (1995) BioTechniques , vol.19 , pp. 780-784
    • Desai, U.J.1    Pfaffle, P.K.2
  • 3
    • 0025633829 scopus 로고
    • Purification of Thermus aquaticus DNA polymerase expressed in Escherichia coli
    • Engelke, D.R., A. Krikos, M.E. Bruck and D. Ginsburg. 1990. Purification of Thermus aquaticus DNA polymerase expressed in Escherichia coli. Anal. Biochem. 191:396-400.
    • (1990) Anal. Biochem. , vol.191 , pp. 396-400
    • Engelke, D.R.1    Krikos, A.2    Bruck, M.E.3    Ginsburg, D.4
  • 4
    • 84967834658 scopus 로고
    • Isolation and properties of the DNA polymerase from extremely thermophilic bacterium Thermus aquaticus YT1
    • Kaledin, A.S., A.G. Slyusarenko and S.I. Gorodetskii. 1980. Isolation and properties of the DNA polymerase from extremely thermophilic bacterium Thermus aquaticus YT1. Biokhimiia 45:494-501.
    • (1980) Biokhimiia , vol.45 , pp. 494-501
    • Kaledin, A.S.1    Slyusarenko, A.G.2    Gorodetskii, S.I.3
  • 5
    • 0027494112 scopus 로고
    • High-level expression, purification, and enzymatic characterization of full-length Thermus aquaticus DNA polymerase and a truncated form deficient in 5′ to 3′ exonuclease activity
    • Lawyer, F.C., S. Stoffel, R.K. Saiki, S-Y. Chang, P.A. Landre, R.D. Abramson and D.H. Gelfand. 1993. High-level expression, purification, and enzymatic characterization of full-length Thermus aquaticus DNA polymerase and a truncated form deficient in 5′ to 3′ exonuclease activity. PCR Methods Appl. 2:275-287.
    • (1993) PCR Methods Appl. , vol.2 , pp. 275-287
    • Lawyer, F.C.1    Stoffel, S.2    Saiki, R.K.3    Chang, S.-Y.4    Landre, P.A.5    Abramson, R.D.6    Gelfand, D.H.7
  • 6
    • 0024560060 scopus 로고
    • Isolation, characterization and expression in Escherichia coli of the DNA polymerase gene from Thermus aquaticus
    • Lawyer, F.C., S. Stoffel, R.K. Saiki, K. Myambo, R. Drummond and D.H. Gelfand. 1989. Isolation, characterization and expression in Escherichia coli of the DNA polymerase gene from Thermus aquaticus. J. Biol. Chem. 264:6427-6437.
    • (1989) J. Biol. Chem. , vol.264 , pp. 6427-6437
    • Lawyer, F.C.1    Stoffel, S.2    Saiki, R.K.3    Myambo, K.4    Drummond, R.5    Gelfand, D.H.6
  • 7
    • 0031841506 scopus 로고    scopus 로고
    • Model phase separations of proteins using aqueous/ethanol components
    • Louwrier, A. 1998. Model phase separations of proteins using aqueous/ethanol components. Biotechnol. Tech. 72:363-365.
    • (1998) Biotechnol. Tech. , vol.72 , pp. 363-365
    • Louwrier, A.1
  • 8
    • 0025814594 scopus 로고
    • Detection of DNA contamination in Taq polymerase
    • Schmidt, T.M., B. Pace and N.R. Pace. 1991. Detection of DNA contamination in Taq polymerase. BioTechniques 11:176-177.
    • (1991) BioTechniques , vol.11 , pp. 176-177
    • Schmidt, T.M.1    Pace, B.2    Pace, N.R.3

* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.